全文获取类型
收费全文 | 379篇 |
免费 | 34篇 |
出版年
2022年 | 3篇 |
2021年 | 5篇 |
2018年 | 4篇 |
2017年 | 9篇 |
2016年 | 5篇 |
2015年 | 16篇 |
2014年 | 19篇 |
2013年 | 25篇 |
2012年 | 21篇 |
2011年 | 13篇 |
2010年 | 10篇 |
2009年 | 15篇 |
2008年 | 17篇 |
2007年 | 11篇 |
2006年 | 14篇 |
2005年 | 13篇 |
2004年 | 10篇 |
2003年 | 7篇 |
2002年 | 8篇 |
2001年 | 18篇 |
2000年 | 16篇 |
1999年 | 9篇 |
1998年 | 12篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1992年 | 3篇 |
1991年 | 6篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1985年 | 3篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1976年 | 6篇 |
1975年 | 4篇 |
1972年 | 2篇 |
1964年 | 2篇 |
1935年 | 3篇 |
1932年 | 2篇 |
1914年 | 2篇 |
1913年 | 2篇 |
1911年 | 4篇 |
1910年 | 2篇 |
1904年 | 2篇 |
1903年 | 2篇 |
1892年 | 2篇 |
排序方式: 共有413条查询结果,搜索用时 93 毫秒
1.
2.
3.
4.
5.
C B Harley J Lawrie M Betlach R Crea H W Boyer J Hedgpeth 《Nucleic acids research》1988,16(15):7269-7285
6.
7.
Elisabetta Zappone Isabelle Dugast Panos Papadopoulos Kelly Theriault Veronique David Jean-Yves LeGall Kim Summers Lawrie Powell Jim Drysdale 《Human genetics》1991,86(6):557-561
Summary This paper addresses the question of whether abnormalities in ferritin expression in the iron storage disease hemochromatosis (HC) involve major deletions or alterations in regions containing the two ferritin H genes that lie near the disease locus on chromosome 6p. We present evidence from analyses of Southern blots that neither gene is deleted in hemochromatosis. We also describe a polymorphism in one of the genes that we have previously shown to be a processed pseudogene. This polymorphism does not correlate with the presence of HC. The PIC value for this polymorphism was calculated as 0.49. 相似文献
8.
Construction, expression, and biologic activity of murine/human chimeric antibodies with specificity for the human alpha/beta T cell receptor. 总被引:2,自引:0,他引:2
C W Shearman E J Kanzy D K Lawrie Y W Li P Thammana G P Moore R Kurrle 《Journal of immunology (Baltimore, Md. : 1950)》1991,146(3):928-935
Murine/human chimeric antibodies with specificity for the human TCR-alpha/beta have been produced by genetic engineering. The L and H chain V region exons encoding the murine mAb BMA 031 were isolated and inserted into mammalian expression vectors containing the human kappa and gamma 1 or gamma 4 C region exons. The chimeric genes were transfected into murine Sp2/O hybridoma cells by electroporation and transfectomas secreting chimeric antibody were isolated. Secretion levels ranged from 1 to 7 pg/cell/24 h. The chimeric antibodies bound specifically to T cells and competed effectively with the parental murine mAb for binding to these sites. The ability to promote antibody-dependent cell-mediated cytolysis was significantly enhanced in the chimeric antibodies as compared with murine BMA 031. C-dependent cytolysis, however, was not detectable with any of the antibodies. Chimeric BMA 031 is a clinically relevant, genetically engineered antibody with potential uses in transplantation, graft-vs-host disease, autoimmune diseases and other T cell-related disorders. 相似文献
9.
The effect of neurotensin on submaximally-stimulated hepatobiliary and pancreatic secretion was studied in 6 healthy subjects. An intravenous infusion of neurotensin 1.4 ± 0.3 pmol/kg/min, designed to reproduce plasma neurotensin immunoreactivity levels within the physiological range, produced a significant increase in pancreatic bicarbonate output. Plasma concentrations of pancreatic polypeptide rose by 83 ± 16 pmol/l and were associated with a small reduction in trypsin, but no significant change in bilirubin outputs. 相似文献
10.
DNA strand specificity of temporal RNA classes produced during infection of Bacillus subtilis by SP82.
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The DNA of the Bacillus subtilis bacteriophage SP82 has been separated into heavy (H) and light (L) fractions by centrifugation in buoyant density gradients in the presence of polyguanylic acid. Competition-hybridization experiments were performed with these separated fractions using RNAs isolated from cells labeled at intervals which account for 80% of the lytic cycle and unlabeled competitor RNAs isolated from phage-infected cells at 2-min intervals throughout infection. The analysis of temporal RNA classes were facilitated by use of a double reciprocal plot of the data. Five temporal classes binding to the H fraction and three binding to the L fraction were detected; the possible existence of an additional class transcribed from the H fraction is discussed. RNA synthesized in the presence of chloramphenicol contains two of the three classes produced from L-DNA and two of the five classes transcribed from H-DNA. 相似文献