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Latiporn Udomsuk Kanokwan Jarukamjorn Hiroyuki Tanaka Waraporn Putalun 《Biotechnology letters》2011,33(2):369-374
The effect of abiotic and biotic elicitors (methyl jasmonate, chitosan, salicylic acid, Agrobacterium, and yeast extract) at various concentrations on total isoflavonoid accumulation was studied in the hairy root cultures of
Pueraria candollei. All elicitors stimulated isoflavonoid production. Yeast extract (0.5 mg/ml) was the most efficient giving total isoflavonoids
at 60 ± 1 mg/g dry wt, which was 4.5-fold higher than control hairy roots on day 3 of elicitation. 相似文献
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Udomsin Orapin Yusakul Gorawit Kraithong Witsarut Udomsuk Latiporn Kitisripanya Tharita Juengwatanatrakul Thaweesak Putalun Waraporn 《Plant Cell, Tissue and Organ Culture》2019,136(1):141-151
Plant Cell, Tissue and Organ Culture (PCTOC) - Pueraria candollei var. mirifica (PM), a rejuvenating herb of Thailand, has been applied efficaciously to relieve symptoms of estrogen deficiency in... 相似文献
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Latiporn Udomsuk Thaweesak Juengwattanatrakul Kanokwan Jarukamjorn Waraporn Putalun 《Acta Physiologiae Plantarum》2012,34(3):1093-1100
Miroestrol and deoxymiroestrol are highly active phytoestrogens derived from the tuberous roots of Pueraria candollei var. mirifica. To date, there have been no reports regarding the production of miroestrol and deoxymiroestrol in in vitro cell culture.
In this study, callus and cell suspension cultures were established for the purpose of investigating miroestrol and deoxymiroestrol
content in P. candollei var. mirifica cells. Stem-derived callus cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg l−1 thidiazuron (TDZ), 0.5 mg l−1 naphthaleneacetic acid (NAA), and 1.0 mg l−1 benzyladenine (BA) provided optimal conditions for the accumulation of deoxymiroestrol and total isoflavonoids. The calli
produced 184.83 ± 20.09 μg g−1 dry weight of total chromene and 20.72 ± 2.38 mg g−1 dry weight of total isoflavonoid. This is the first report to suggest that callus culture is a suitable alternative method
for producing miroestrol and deoxymiroestrol. Carbon sources were evaluated for the cell suspension cultures of P. candollei var. mirifica. Sucrose provided optimal conditions for biomass production, whereas fructose was the most suitable carbon source for deoxymiroestrol
and isoflavonoid production. The information from our study can be employed for enhancing the production of miroestrol, deoxymiroestrol,
and total isoflavonoids using in vitro cell culture of P. candollei var. mirifica. 相似文献
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