They're All My Children: Foster Mothering in America

They're All My Children: Foster Mothering in America. Danielle F. Wozniak. New York: NYU Press, 2002. + 245 pp.     

immunocytochemical localization of urokinase-type plasminogen activator in lewis lung carcinoma

The invasively growing and metasizing Lewis lung carcinoma consistently contained urokinase-type plasminogen activator (u-PA) enzyme activity. When investigated immunocytochemically with antibodies against u-PA, different parts of individual tumors showed a pronounced heterogeneity in staining intensity. Strong staining was found in areas with invasive growth and degradation of surrounding normal tissue, while other areas were completely devoid of staining. Immunoreactivity occurred both with a perinuclear cytoplasmic localization in tumor cells and associated with apparently extracellular material. SDS PAGE of tumor extracts, under both reducing and nonreducing conditions, followed by immunoblotting, showed only one immunocytochemically stainable band with an electrophoretic mobility corresponding to that of purified proenzyme to u-PA, while no two-chain u-PA was detected. This indicates that the major part of the activator in Lewis lung carcinoma is present as one-chain pro-u-PA.     

Use of substances stimulating enterobacteria growth in obtaining microbial adsorbents

The combination of substances, capable of stimulating the growth of enterobacteria and consisting of reducing agents (sodium thiosulfate and sodium sulfate), the vitamin preparation of yeast extract, polyatomic alcohol (glycerol) and ion exchange resin AB-17-8, is proposed. The addition of this combination of substances to culture media based on clots of animal blood has made it possible to achieve more intensive growth of Escherichia and Proteus than on classical meat culture media.