全文获取类型
收费全文 | 1262篇 |
免费 | 120篇 |
专业分类
1382篇 |
出版年
2023年 | 12篇 |
2022年 | 15篇 |
2021年 | 28篇 |
2020年 | 14篇 |
2019年 | 12篇 |
2018年 | 25篇 |
2017年 | 20篇 |
2016年 | 42篇 |
2015年 | 54篇 |
2014年 | 71篇 |
2013年 | 87篇 |
2012年 | 94篇 |
2011年 | 110篇 |
2010年 | 73篇 |
2009年 | 52篇 |
2008年 | 79篇 |
2007年 | 60篇 |
2006年 | 78篇 |
2005年 | 45篇 |
2004年 | 39篇 |
2003年 | 32篇 |
2002年 | 37篇 |
2001年 | 20篇 |
2000年 | 28篇 |
1999年 | 28篇 |
1998年 | 10篇 |
1997年 | 6篇 |
1996年 | 9篇 |
1995年 | 9篇 |
1994年 | 6篇 |
1992年 | 11篇 |
1991年 | 11篇 |
1990年 | 15篇 |
1989年 | 10篇 |
1988年 | 10篇 |
1987年 | 9篇 |
1986年 | 8篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 11篇 |
1976年 | 5篇 |
1975年 | 4篇 |
1974年 | 12篇 |
1973年 | 10篇 |
1967年 | 7篇 |
排序方式: 共有1382条查询结果,搜索用时 15 毫秒
1.
2.
F. Fontaine E. Kiefer C. Clément M. Burrus J. L. Druelle 《Trees - Structure and Function》1999,14(2):83-90
In the present work, we described the fate of proventitious epicormic buds on the trunks of 40-year-old Quercus petraea trees and in parallel the vascular trace they produced in the wood. Our results show that small and large individual epicormic
buds can survive as buds for 40 years and that both are composed of a terminal meristem and scales. Meristematic areas are
detected in the scale axils of small buds; in addition to these meristems the large buds also have secondary bud primordia.
The small buds are connected to the pith of the main stem by a unique trace, whereas the large buds are connected by one or
multiple traces. A single trace might imply that the whole bud is still alive and multiple traces might indicate that the
terminal meristem has died. In the latter case, each trace is connected to a secondary bud of the large bud. The buds found
in a cluster are composed of a terminal meristem and scales with axillary meristems in the scale axils. A cluster is connected
to the pith of a stem either by a unique trace when it seems to be the result of partial abscission of an epicormic shoot
or multiple traces when it might have originated from an epicormic bud in which the terminal meristem has died. Whatever the
type of the bud, the vascular trace in the bark is composed of a cambium, secondary xylem and parenchyma cells and the trace
present in the wood had parenchyma cells with vestiges of secondary xylem. Each year, the vascular trace should be produced
in the bark by the cambium of the tree but not by the bud itself. On 40-year-old Q. petraea, we observed a proliferation of epicormic buds and in parallel a multiplication of the number of vascular traces in the trunk,
but the knots caused by the traces of epicormic buds in the wood, either as individuals or in clusters, are minor since their
colours are only slightly darker than those of woody rays and they are less than 2 mm in diameter. The knots will appear when
epicormic buds develop into shoots.
Received: 30 March 1999 / Accepted: 09 June 1999 相似文献
3.
Studies aiming to predict the impact on marine life of ocean acidification and of altered salinity have shown altered development in various species including sea urchins. We have analyzed how external Na, Ca, pH and bicarbonate control the first mitotic divisions of sea urchin embryos. Intracellular free Ca (Cai) and pH (pHi) and the activities of the MAP kinase ERK and of MPF regulate mitosis in various types of cells including oocytes and early embryos. We found that intracellular acidification of fertilized eggs by Na-acetate induces a huge activation of ERK at time of mitosis. This also stops the cell cycle and leads to cell death, which can be bypassed by treatment with the MEK inhibitor U0126. Similar intracellular acidification induced in external medium containing low sodium or 5-(N-Methyl-N-isobutyl) amiloride, an inhibitor of the Na+/H+ exchanger, also stops the cell cycle and leads to cell death. In that case, an increase in Cai and in the phosphorylation of tyr-cdc2 occurs during mitosis, modifications that depend on external Ca. Our results indicate that the levels of pHi and Cai determine accurate levels of Ptyr-Cdc2 and P-ERK capable of ensuring progression through the first mitotic cycles. These intracellular parameters rely on external Ca, Na and bicarbonate, alterations of which during climate changes could act synergistically to perturb the early marine life. 相似文献
4.
Marjorie Fanjul Laetitia Alvarez Etienne Hollande 《The journal of histochemistry and cytochemistry》2007,55(8):783-794
The high intraluminal concentrations of HCO(3)(-) in the human pancreatic ducts have suggested the existence of a membrane protein supplying the Cl(-)/HCO(3)(-) exchanger. Membrane-bound carbonic anhydrase IV (CA IV) is one of the potential candidates for this protein. The difficulties in isolating human pancreatic ducts have led the authors to study the molecular mechanisms of HCO(3)(-) secretion in cancerous cell lines. In this work, we have characterized the CA IV expressed in Capan-1 cells. A 35-kDa CA IV was detected in cell homogenates and purified plasma membranes. Treatment of purified plasma membranes with phosphatidylinositol-phospholipase-C indicated that this CA IV was not anchored by a glycosylphosphatidylinositol (GPI). In contrast, its detection on purified plasma membranes by an antibody specifically directed against the carboxyl terminus of human immature GPI-anchored CA IV indicated that it was anchored by a C-terminal hydrophobic segment. Immunoelectron microscopy and double-labeling immunofluorescence revealed that this CA IV was present on apical plasma membranes, and in the rough endoplasmic reticulum, the endoplasmic reticulum-Golgi intermediate compartment, the Golgi complex, and secretory granules, suggesting its transport via the classical biosynthesis/secretory pathway. The expression in Capan-1 cells of a 35-kDa CA IV anchored in the apical plasma membrane through a hydrophobic segment, as is the case in the healthy human pancreas, should make the study of its role in pancreatic HCO(3)(-) secretion easier. 相似文献
5.
F Fontaine F Vasseur J B Savary M Menais M Roussel M M Deminatti 《Annales de génétique》1988,31(2):102-104
Prenatal diagnosis of cystic fibrosis was performed with linked DNA probes in a couple with a 1:4 risk. The limits and the future of molecular prenatal diagnosis are discussed. 相似文献
6.
7.
Livia Poenaru Laetitia Castelnau Agnès Choiset Y. Rouquet F. Thepot 《Human genetics》1985,69(4):378-379
Summary Fourteen lysosomal enzymes were compared in 20 cultured cell lines from chorionic biopsy and corresponding embryonic tissue after voluntary abortions. Enzymatic expression appears to be similar in cultured cells from both sources with some slightly higher levels for chorionic villi. We stress the importance of culturing chorionic villi especially in the case of enzymes (-L-iduronidase) or diseases (I cell disease) whose expression is unusual in fresh trophoblast tissue. 相似文献
8.
9.
Interferon and murine leukemia. I. Inhibitory effect of interferon preparations on development of friend leukemia in mice 总被引:10,自引:0,他引:10
10.