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1.
Significant seasonal changes in the concentrations of N, P, K, and Ca were observed in the herb layer vegetation at Nairobi National Park and Masai Mara Game Reserve in Kenya. These changes were related to the alternation of wet and dry seasons. Live forbs typically had the highest concentrations of all nutrients, especially N and Ca, at both locations. Standing dead and litter compartments showed less seasonal variation and had the lowest concentrations of all nutrients except Ca when compared with those of other compartments. Standing stocks of all nutrients were higher at Nairobi National Park than at Masai Mara due to larger litter and standing dead compartments resulting from lower grazing intensity and a lower frequency of fire. Most of the aboveground nutrients at Nairobi National Park appeared to be recycled through the decomposer pathway, while a large proportion of the standing stock at Masai Mara appeared to be recycled through the excreta of large mammalian herbivores and through fires. The crude protein content of the live grass compartment fell below maintenance levels required for ruminants (5% crude protein) during September 1980 and February 1981 at Nairobi National Park and during June and July 1980 at Masai Mara Game Reserve. Thus, herbivore populations in these preserves may be limited by shortages of nutritionally adequate food during dry seasons, as previously reported for other regions in East Africa.  相似文献   
2.
SYNOPSIS The development of 1st generation schizonts of Eimeria callospermophili was studied with cell cultures and with experimentally infected host animals, Spermophilus armatus. Sporozoite-shaped schizonts each had 5-10 nuclei and all of the organelles of the sporozoite; each nucleus had a nucleolus and an associated Golgi apparatus. In stages immediately preceding merozoite formation, an intranuclear spindle apparatus with conical polar areas were observed near the outer margin of each nucleus. Two centrioles, each having 9 single peripheral tubules and one central tubule, were observed near each pole in some specimens. Merozoite formation began internally, with anlagen of 2 merozoites developing near each nucleus. The inner membrane of the merozoites first appeared as 2 dense thickenings adjacent to the polar cones and centrioles; subpellicular microtubules appeared simultaneously. Two anterior annuli and the conoid formed between the 2 thickenings. Vesicles, possibly of Golgi origin, were located next to the forming inner membrane. As the forming merozoites underwent elongation, a rhoptries anlage, a Golgi apparatus, refractile bodies, and mitochondria were incorporated into each. Sporozoite-shaped schizonts with merozoite anlagen transformed into spheroid or ovoid schizonts; at this time the conoid, rhoptries, micronemes, and the inner membrane of the pellicle gradually disappeared; several small refractile bodies were formed from the larger one. When development was about 1/3 complete, the immature merozoites began to grow outward from the surface of the schizont. In this phase of development, the single surface membrane of the schizont became the outer membrane of the merozoite's pellicle, and additional organelles, including the nucleus, were incorporated. Finally, the merozoites became pinched off, leaving a residual body. Development in cell cultures and host tissues was similar. This type of schizogony, previously undescribed in Eimeria, is compared with corresponding stages of development in other species of Eimeria and Sporozoa.  相似文献   
3.
ABSTRACT. Expression of a 21 kDa determinant (Pbs21), first detected on the surface of ookinetes, and of the circumsporozoite protein (CSP) was studied by immunofluorescence and Western blots during the developmental cycle of Plasmodium berghei in the mosquito A nopheles stephensi . The expression of Pbs21 was predominantly localised on the ookinete surface one day after the infectious blood meal, and thereafter reactivity declined to a minimum on days 2 and 3, the time of onset of oocyst development. A gradual increase in fluorescence was observed on the oocysts from day 6 that was retained until day 17 post-infection. In contrast, sporozoites released from oocysts or salivary glands showed little or no antibody labelling with anti-Pbs21. Circumsporozoite protein was not detectable in any rnidgut preparations until 5–6 days after feeding, when reactivity was observed against immature oocysts. Expression then continued and increased throughout oocyst and sporozoite development. Western blots confirmed that Pbs21 was expressed minimally during the oocyst development but was not detectable in sporozoites. Co-localisation of anti-Pbs21 and anti-CSP monoclonal antibodies to the 50 kDa and 60 kDa bands in Western blots of sporozoite suggests immunological cross-reactivity between the CSP and the anti-21 kDa antibodies.  相似文献   
4.
Seabird associations in Pacific equatorial waters   总被引:2,自引:0,他引:2  
Seabirds were counted along the same cruise tracks in the eastern tropical Pacific (10°øN-10°øS and 140°ø-90°øW) each spring and autumn for 8 years, 1984–1991. During spring, three species groups were apparent among eight species seen in five or more years: (1) Leach's Storm-petrel Oceanodroma leucorhoa and Wedge-rumped Storm-petrel Oceanodroma tethys , which feed on neuston by surface seizing; (2) Black-winged Petrel Pterodroma nigripennis and White-winged Petrel Pterodroma leucoptera , which feed on neuston and micronekton by surface seizing; (3) Juan Fernandez Petrel Pterodroma externa , Wedge-tailed Shearwater Puffinus pacificus and Sooty Tern Sterna fuscata , which feed on micronekton driven to the surface by foraging tuna. During autumn, only one group was identified from the seven species seen in five or more years: Leach's Storm-petrel associated with either Wedge-rumped Storm-petrel or Juan Fernandez Petrel and/or White-winged Petrel. Annual variability in assemblage composition was more extreme in the autumn and thus was similar to variation in physical and biological factors linked to the El Niño-Southern Oscillation cycle.  相似文献   
5.
Cells of the ciliate Tetrahymena pyriformis GL overproduce and accumulate massive quantities of the heme intermediate, protoporphyrin IX. Protoporphyrin is localized intracellularly in discrete membranous compartments. The amount of porphyrin stored in the cell changes dramatically as cells progress through the growth cycle. Porphyrin overproduction is stimulated by δ-aminolevulinic acid, but only during the mid-stationary phase. Overproduction of protoporphyrin IX apparently results from an increase, late in the growth cycle, of activities subsequent to δ-aminolevulinic acid synthetase. Feedback inhibition in the pathway by accumulated protoporphyrin IX does not occur. The presence of Co2+ completely inhibits accumulation of protoporphyrin IX in a manner reversed by δ-aminolevulinic acid. Sn4+ stimulates protoporphyrin IX accumulation in the culture.  相似文献   
6.
Axenic crown gall tumor callus (from Vinca rosea L.) which is known to synthesize its own auxin is able to convert exogenous 14C-indole or tryptamine to indoleacetic acid [5.4 and 10 × 10−6μmol × h−1× (g fr wt)−1 respectively], but little or no 3H-tryptophan is converted [less than 6.4 × 10−8×μmol × h−1× (g fr wt)−1].  相似文献   
7.
The expression of the five muscarinic acetylcholine receptor (mAChR) subtypes (m1–m5) in the vestibular end-organs and in the primary afferent vestibular ganglia of the human and rat was studied using RT-PCR from the two tissue populations from both species. In the human, although all five mAChR subtypes were expressed in brain, only the m1, m2, and m5 mAChR subtypes were amplified from both the vestibular ganglia and the vestibular end-organs, while in the rat, all five mAChR subtypes were expressed. These data suggest that the efferent cholinergic axo-dendritic and axo-somatic synapses have a muscarinic component and that there are pharmacologic implications for patients with vestibular dysfunction.  相似文献   
8.
1. The influence of coarse substratum and flow, coarse substratum and food, and predation risk and flow on habitat use by Gammarus pulex was studied in three experiments conducted in artificial stream channels. Each stream channel consisted of a riffle and pool habitat.
2. Location of coarse substrata and food was manipulated by placing cobbles (coarse substratum) and leaf packs (food) in different habitats. Predation risk was varied by running experiments in the presence and absence of sculpins ( Cottus gobio ), and flow was varied by pumping water with one or two pumps.
3. In all experiments Gammarus were most abundant in pools but placement of cobbles in riffles increased use of the latter. An even greater percentage of Gammarus used riffles if leaf packs were also placed there. Decreased discharge and the presence of sculpins ( Cottus gobio ) also caused Gammarus to increase use of riffles. These data indicate that Gammarus is able to evaluate differences in habitat quality and respond accordingly.  相似文献   
9.
10.
Reproductive traits of Drosophila hibisci collected at 18 sites in the Northern Territory (NT) of Australia in May, 1998, as well as at two sites in north Queensland, in June, 1998, were compared to those from earlier work on a cline in ovariole number in D. hibisci along the east coast of Australia. The flies in the NT were considerably smaller, but had more ovarioles than comparably-sized flies on the east coast. Although the flies on the east coast showed an increasing number of ovarioles in populations at increasing distances from the equator, these new populations, both on the east coast and in the NT, reversed this trend, producing a generally U-shaped pattern of ovariole number with latitude among all populations. The northernmost and southernmost populations allocate more to ovariole numbers than populations in intermediate latitudes. Ovariole number is closely related to body size of females in all populations, but the regression coefficient is small at intermediate latitudes and increases at the northern and southern ends of the distribution. Egg volumes primarily varied with body size of the female (positive) and number of ovarioles per female (negatively), producing a generally inverted U-shaped pattern of egg volumes with latitude. Reproductive allocation patterns, but not thorax size or ovariole number, varied significantly in two samples taken 10 days apart at one NT site. This variation probably results from environmental differences across generations of developing larvae and is consistent with our earlier suggestion of substantial effects of the environment, primarily rainfall and temperature, on reproductive allocation in D. hibisci.  相似文献   
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