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Choi Woo-Jung Lee Sang-Mo Ro Hee-Myong Kim Kyoung-Cheol Yoo Sun-Ho 《Plant and Soil》2002,245(2):223-232
To investigate the effect of inorganic fertilizer and composted manure amendments on the N isotope composition (delta
15N) of crop and soil, maize (Zea mays L.) was cultivated under greenhouse conditions for 30, 40, 50, 60, and 70 days. Composted pig manure (delta
15N= +13.9) and urea (-2.3) were applied at 0 and 0 kg N ha–1 (C0U0), 0 and 150 kg N ha–1 (C0U2), 150 and 0 kg N ha–1 (C2U0), and 75 and 75 kg N ha–1 (C1U1), respectively. The delta
15N of total soil-N was not affected by both amendments, but delta
15N of NH+
4 and NO–
3 provided some information on the N isotope fractionation in soil. During the early growth stage, significant differences (P < 0.05) in delta
15N among maize subjected to different treatments were observed. After 30 days of growth, the delta
15N values of maize were +6.6 for C0U0, +1.1 for C0U2, +7.7 for C2U0, and +4.5 for C1U1. However, effects of urea and composted manure application on maize delta
15N progressively decreased with increasing growth period, probably due to isotope fractionation accompanying N losses and increased uptake of soil-derived N by maize. After 70 days of growth, delta
15N of leaves and grains of maize amended with composted pig manure were significantly (P < 0.05) higher than those with urea. The temporal variations in delta
15N of maize amended with urea and composted manure indicate that plant delta
15N is generally not a good tracer for N sources applied to field. Our data can be used in validation of delta
15N fractionation models in relation to N source inputs. 相似文献
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Kyoung-Cheol Kim Si-Wouk Kim Myong-Jun Kim Seong-Jun Kim 《Biotechnology and Bioprocess Engineering》2005,10(1):52-59
The study was targeted to saccharify foodwastes with the cellulolytic and amylolytic enzymes obtained from culture supernatant
ofTrichoderma harzianum FJ1 and analyze the kinetics of the saccharification in order to enlarge the utilization in industrial application.T. harzianum FJ1 highly produced various cellulolytic (filter paperase 0.9, carboxymethyl cellulase 22.0, β-glucosidase 1.2, Avicelase
0.4, xylanase 30.8, as U/mL-supernatant) and amylolytic (α-amylase 5.6, β-amylase 3.1, glucoamylase 2.6, as U/mL-supernatant)
enzymes. The 23–98 g/L of reducing sugars were obtained under various experimental conditions by changing FPase to between
0.2–0.6 U/mL and foodwastes between 5–20% (w/v), with fixed conditions at 50°C, pH 5.0, and 100 rpm for 24 h. As the enzymatic
hydrolysis of foodwastes were performed in a heterogeneous solid-liquid reaction system, it was significantly influenced by
enzyme and substrate concentrations used, where the pH and temperature were fixed at their experimental optima of 5.0 and
50°C, respectively. An empirical model was employed to simplify the kinetics of the saccharification reaction. The reducing
sugars concentration (X, g/L) in the saccharification reaction was expressed by a power curve (X=K·t
n) for the reaction time (t), where the coefficient,K andn, were related to functions of the enzymes concentrations (E) and foodwastes concentrations (S), as follow:K=10.894 Ln(E·S
2)-56.768,n=0.0608·(E/S)−0.2130. The kinetic developed to analyze the effective saccharification of foodwastes composed of complex organic compounds could
adequately explain the cases under various saccharification conditions. The kinetics results would be available for reducing
sugars production processes, with the reducing sugars obtained at a lower cost can be used as carbon and energy sources in
various fermentation industries. 相似文献
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