On the Feasibility of Plasma Separation of Spent Nuclear Fuel Components in a Nonuniform Magnetic Field

Plasma Physics Reports - A new concept of plasma separation of spent nuclear fuel components in a variable-cross-section chamber with a nonuniform magnetic field is proposed. Numerical simulation...     

Patient-specific stem cell lines derived from human parthenogenetic blastocysts

Parthenogenetic activation of human oocytes may be one way to produce histocompatible cells for cell-based therapy. We report the successful derivation of six pluripotent human embryonic stem cell (hESC) lines from blastocysts of parthenogenetic origin. The parthenogenetic human embryonic stem cells (phESC) demonstrate typical hESC morphology, express appropriate markers, and possess high levels of alkaline phosphatase and telomerase activity. The phESC lines have a normal 46, XX karyotype, except one cell line, and have been cultured from between 21 to 35 passages. The phESC lines form embryoid bodies in suspension culture and teratomas after injection to immunodeficient animals and give differentiated derivatives of all three embryonic germ layers. DNA profiling of all six phESC lines demonstrates that they are MHC matched with the oocyte donors. The study of imprinted genes demonstrated further evidence of the parthenogenetic origin of the phESC lines. Our research has resulted in a protocol for the production of human parthenogenetic embryos and the derivation of stem cell lines from them, which minimizes the presence of animal-derived components, making the derived phESC lines more suitable for potential clinical use.     

The typological structure of hydrophilic flora and vegetation of techogenically transformed lakes of Vologda Poozerye

The typological elements of the hydrophilic component of the vegetative cover of lakes of Vologda Poozerye (hydrophyton, hygrophyton, etc.), their taxonomic composition, and the cenotic structure of communities were analyzed. The relationship between hydrophyte communities and different types of water bodies was shown. The hydrophilic vegetation is represented by 5 classes, 9 orders, 12 unions, and 36 associations. Characteristics of the communities are provided.     

Structure of hydrophilic vegetation from the littoral zone of the Middle Caspian Sea

The structure of hydrophilic vegetation from the littoral zone of the Kizlyar Bay has been analyzed. The following typological complexes have been determined: halohydrophyton, halohygrophyton, halopaludophyton, and psammohydrophiton. The halohydrophyton and halohygrophyton formations are characterized.     

Role of the Sin3-Histone Deacetylase Complex in Growth Regulation by the Candidate Tumor Suppressor p33ING1

Sin3 is an evolutionarily conserved corepressor that exists in different complexes with the histone deacetylases HDAC1 and HDAC2. Sin3-HDAC complexes are believed to deacetylate nucleosomes in the vicinity of Sin3-regulated promoters, resulting in a repressed chromatin structure. We have previously found that a human Sin3-HDAC complex includes HDAC1 and HDAC2, the histone-binding proteins RbAp46 and RbAp48, and two novel polypeptides SAP30 and SAP18. SAP30 is a specific component of Sin3 complexes since it is absent in other HDAC1/2-containing complexes such as NuRD. SAP30 mediates interactions with different polypeptides providing specificity to Sin3 complexes. We have identified p33ING1b, a negative growth regulator involved in the p53 pathway, as a SAP30-associated protein. Two distinct Sin3-p33ING1b-containing complexes were isolated, one of which associates with the subunits of the Brg1-based Swi/Snf chromatin remodeling complex. The N terminus of p33ING1b, which is divergent among a family of ING1 polypeptides, associates with the Sin3 complex through direct interaction with SAP30. The N-terminal domain of p33 is present in several uncharacterized human proteins. We show that overexpression of p33ING1b suppresses cell growth in a manner dependent on the intact Sin3-HDAC-interacting domain.     

Investigation of the Influence of Injection Parameters on Particles Motion in Electric and Magnetic Fields for Designing Plasma Separation Technique

Plasma Physics Reports - The paper continues studies of the capabilities of plasma treatment of spent nuclear fuel and radioactive waste. The study is devoted to the problem of integration of the...     

NMR Dynamics of Transmembrane and Intracellular Domains of p75NTR in Lipid-Protein Nanodiscs

P75NTR is a type I integral membrane protein that plays a key role in neurotrophin signaling. However, structural data for the receptor in various functional states are sparse and controversial. In this work, we studied the spatial structure and mobility of the transmembrane and intracellular parts of p75NTR, incorporated into lipid-protein nanodiscs of various sizes and compositions, by solution NMR spectroscopy. Our data reveal a high level of flexibility and disorder in the juxtamembrane chopper domain of p75NTR, which results in the motions of the receptor death domain being uncoupled from the motions of the transmembrane helix. Moreover, none of the intracellular domains of p75NTR demonstrated a propensity to interact with the membrane or to self-associate under the experimental conditions. The obtained data are discussed in the context of the receptor activation mechanism.