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1.
P K Vlasov G T Kilosanidze D L Ukrainski? A V Kuz'min V G Tumanian N G Esipova 《Biofizika》2001,46(3):573-576
Regions of left-handed polyproline II type conformation in globular proteins were studied throughout the PDB bank. The length and sequence of corresponding fragments were analyzed. It was found that a lot of tetrapeptides (from combinatorial possible ones) show the tendency to be included in the left-handed helices. Much more tetrapeptides do not occur in this structure type. 相似文献
2.
ALAIN THIÉRY NICOLAS RABET GABRIEL NÈVE 《Biological journal of the Linnean Society. Linnean Society of London》2007,90(1):55-60
The shape of the resting eggs of a large branchiopod crustacean, the Anostraca Tanymastix stagnalis , is represented very accurately by analytical expressions. The occurrence of atypical shape of some T. stagnalis eggs may be viewed as a simple change of the analytical expression describing the usual egg shape. Their unusual shape may be explained by a higher embryo volume within an envelope of a given size. Biological implications are briefly discussed and hypothesized in an evolutionary point of view. © 2007 The Linnean Society of London, Biological Journal of the Linnean Society , 2007, 90 , 55–60. 相似文献
3.
A I Kuz'min S E Khal'chitski? B V Skriabin O K Kaboev E I Shvarts 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1991,(8):6-8
The methods for enzymatic DNA amplification in vitro that allow to avoid the step of preliminary DNA extraction and purification are proposed. Lysates of blood cells in the solution or immobilized on the nylon membrane filters and dried blood spots on the filter paper blotters were used directly in amplification permitting one to solve the problems of adapting the method of polymerase chain reaction in clinical practice, for instance, in massive screening of genome mutations, viral infections etc. 相似文献
4.
A recA-like gene from Pseudomonas aeruginosa was cloned and identified by means of interspecific complementation of gene recA repair defect in Escherichia coli. The gene was mapped in the PvuII-HindIII Ps. aeruginosa chromosome fragment of 1.5 kbp in length. Having been recloned in pUC18 or 19 plasmids in either of possible orientations, this fragment was shown to complement three different defects of E. coli recA mutants: in repair, recombination and SOS functions. 相似文献
5.
A N Kravets M V Zakharova A S Solonin N P Kuz'min V I Taniashin L I Glatman A F Moroz A A Baev 《Molekuliarnaia biologiia》1990,24(2):438-447
A number of recombinant plasmids, containing EcoRV restriction-modification genes have been constructed. Individual genes of this system were introduced into plasmids of various incompatibility groups. Promoter regions of genes encoding methylase and restrictase have been cloned and studied. With the use of specialized vector pVE8 it was shown that the efficiency of the endonuclease gene promoter is comparable with early lambda phage promoters and produced about 70% of PL efficiency. The efficiency of the methylase gene promoter region was twice less than the efficiency of the restriction endonuclease gene promoter. Plasmid with restriction endonuclease gene promoter located downstream in relation to the additional regulatable phage lambda promoter PL has been obtained. It enabled us to construct strains 30-40 fold overproducing this enzyme under conditions of inactivation of the temperature sensitive phage repressor c1857. This construction directs the production of a high level (10%) of the total cellular soluble proteins) of the EcoRV restriction enzyme. The factors that influenced the level of enzyme synthesis under induction are discussed. 相似文献
6.
Abstract.
- 1 The structure of local populations of a monophagous butterfly, the bog fritillary Proclossiana eunomia, was studied in a complex of suitable habitat patches separated by spruce plantations or fertilized pasture.
- 2 An unexpected high level of adult movements between habitat patches was detected by a mark—release—recapture technique. Local populations were connected by adult movements across unsuitable habitats, leading to a meta-population structure.
- 3 This evidence of the metapopulation structure of a specialist butterfly challenges the supposed relationship between habitat specialization and closed, isolated populations.
- 4 Males and females of P.eunomia exhibited different spatial behaviours; females were more likely to emigrate and dispersed further than males. These differences in spatial behaviour are related to the mating system.
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9.
Kuz'min AI Kalenikova EI Zaretskaia MV Zaretskiĭ DV Zakharova NV Medvedev OS Chazov EI 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》1999,85(7):867-877
Physostigmine and an 1-hour immobilisation stress similarly affected functions of the sympatho-adrenal and cardiovascular systems activating the catecholamine secretion and increasing the blood pressure. Yohimbine potentiated the secretory effect but did not change the pressor effect. Intermediate administration of atropine completely eliminated both effects of physostigmine but, being administered prior to the immobilisation, it potentiated the secretory response without affecting the pressor response. The findings reveal a difference in central cholinergic mechanisms of neurohumoral and haemodynamic responses to physostigmine and stress. 相似文献
10.
Kuz'min IV Sidorov GN Botvinov AD Rekhov EI 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2001,(3):28-35
The analysis of the epizootic situation in rabies in the south of Western Siberia during the period of 1990-1999 was carried out on the basis of the analysis of statistical data, field observations and virological investigations. Foxes were found to be the principle virus host and in the steppe areas they probably shared this role with corsac foxes. No data on other orders of mammals taking part in the virus circulation were obtained. The foxes population structure of the territory of the above-mentioned region were studied. The epizootic process was shown to have a cyclic character which corresponds to the models, proposed by foreign researchers for Western Europe. The effectiveness of different methods used for the control of rabies among wild animals was considered. As shown by this study, due to the population ecology of foxes and corsac foxes on the above-mentioned territory the best effect should be expected from measures aimed at frequency limitation of these animals, and not from vaccinal prophylaxis. As an alternative, the possibility of non-interference in the circulation of the virus within the limits of the natural focus in combination with timely measures for the protection of domestic and agricultural animals, as well as humans, from infection was considered. 相似文献