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Inactivation of Salmonella typhi by high levels of volatile fatty acids during anaerobic digestion 总被引:1,自引:0,他引:1
Survival of Salmonella typhi was investigated in an anaerobic digester for cattle dung with volatile fatty acid (VFA) levels of 5000 mg l(-1) and pH 6.0. The organism was added to the digester only once in the first experiment and daily in the other. Survival was monitored on alternate days. In the single dose experiment, the counts of Salm. typhi declined rapidly and the pathogen was completely eliminated within 12 d in the experimental digester (VFA ca 5000 mg l(-1) and pH 6.0), whereas 26 d were required in the control digester (VFA ca 100 mg l(-1) and pH 6.8). T90 values for the experimental and control digesters were 2.44 d and 4.80 d, respectively. In the daily dose experiment, a four log reduction in the pathogen count was observed in the experimental digester, but only a two log reduction in the control digester at the end of the experimental period. The mean T90 values for the experimental and the control digester were 4.22 d and 18.63 d, respectively. In both the experiments, statistical analysis of the data showed significant differences in the survival pattern of Salm. typhi in the two digesters. 相似文献
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Pedro M. Martin-Sanchez Anna A. Gorbushina Hans-Jörg Kunte Jörg Toepel 《Biofouling》2016,32(6):635-644
A wide variety of fungi and bacteria are known to contaminate fuels and fuel systems. These microbial contaminants have been linked to fuel system fouling and corrosion. The fungus Hormoconis resinae, a common jet fuel contaminant, is used in this study as a model for developing innovative risk assessment methods. A novel qPCR protocol to detect and quantify H. resinae in, and together with, total fungal contamination of fuel systems is reported. Two primer sets, targeting the markers RPB2 and ITS, were selected for their remarkable specificity and sensitivity. These primers were successfully applied on fungal cultures and diesel samples demonstrating the validity and reliability of the established qPCR protocol. This novel tool allows clarification of the current role of H. resinae in fuel contamination cases, as well as providing a technique to detect fungal outbreaks in fuel systems. This tool can be expanded to other well-known fuel-deteriorating microorganisms. 相似文献
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Helga Kunte 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1960,43(3):341-358
Zusammenfassung Der Tryptophanabbau in der Leber von Ratte (Mus rattus L. domest.), Kaninchen (Oryctolagus cuniculus L. domest.), Hahn (Gallus bankiva domest.), Taube (Columba livia L.), Frosch (Rana temporaria L.) und Fisch (Leuciscus rutilus L.) wurde in qualitativer und quantitativer Hinsicht untersucht. Es wurden die Aktivitäten des TryptophanPeroxydase-Systems, der Kynureninase und der Kynurenin-Transaminase bestimmt, sowie ihre Verteilung auf die einzelnen Zellfraktionen. Die Papierchromatographie wurde zur qualitativen Analyse der entstandenen Stoffwechselprodukte herangezogen.Außer beim Hahn wird Tryptophan praktisch ausschließlich über Kynurenin zu Kynurensäure und Anthranilsäure abgebaut. Die Verteilung der Enzyme auf die Zellfraktionen entspricht der vom Säugetier bekannten, die Aktivitäten liegen in der gleichen Größenordnung.Beim Hahn wird Kynurenin auch von Mitochondrien und Kernfraktion gebildet. Die Kynureninbildung im Cytoplasma wird durch Kombination von Cytoplasma + Mikrosomen oder Cytoplasma + Mitochondrien auf das Mehrfache erhöht. Auch scheint beim Hahn neben der Kynureninbildung noch ein zweiter Mechanismus des Tryptophanabbaus vorhanden zu sein. Durch Cyclophorasesystem wird aus Tryptophan eine gelbe Substanz gebildet, die aber nicht identifiziert werden konnte. Eine Kynurensäurebildung wird beim Hahn nicht gefunden, was im Einklang steht mit der Tatsache, daß er keine Kynurensäure ausscheidet.Fütterungsversuche an Ratten ergaben, daß bei tryptophanarmer Nahrung die Aktivität des Tryptophan-Peroxydase-Systems deutlich unter die Normalwerte absinkt, während Kynureninase und Kynurenintransaminase nicht beeinflußt werden. 相似文献
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Kunte K 《Proceedings. Biological sciences / The Royal Society》2008,275(1643):1617-1624
Theoretical and empirical observations generally support Darwin's view that sexual dimorphism evolves due to sexual selection on, and deviation in, exaggerated male traits. Wallace presented a radical alternative, which is largely untested, that sexual dimorphism results from naturally selected deviation in protective female coloration. This leads to the prediction that deviation in female rather than male phenotype causes sexual dimorphism. Here I test Wallace's model of sexual dimorphism by tracing the evolutionary history of Batesian mimicry-an example of naturally selected protective coloration-on a molecular phylogeny of Papilio butterflies. I show that sexual dimorphism in Papilio is significantly correlated with both female-limited Batesian mimicry, where females are mimetic and males are non-mimetic, and with the deviation of female wing colour patterns from the ancestral patterns conserved in males. Thus, Wallace's model largely explains sexual dimorphism in Papilio. This finding, along with indirect support from recent studies on birds and lizards, suggests that Wallace's model may be more widely useful in explaining sexual dimorphism. These results also highlight the contribution of naturally selected female traits in driving phenotypic divergence between species, instead of merely facilitating the divergence in male sexual traits as described by Darwin's model. 相似文献
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David W. Kikuchi William L. Allen Kevin Arbuckle Thomas G. Aubier Emmanuelle S. Briolat Emily R. Burdfield-Steel Karen L. Cheney Klára Daňková Marianne Elias Liisa Hämäläinen Marie E. Herberstein Thomas J. Hossie Mathieu Joron Krushnamegh Kunte Brian C. Leavell Carita Lindstedt Ugo Lorioux-Chevalier Melanie McClure Callum F. McLellan Iliana Medina Viraj Nawge Erika Páez Arka Pal Stano Pekár Olivier Penacchio Jan Raška Tom Reader Bibiana Rojas Katja H. Rönkä Daniela C. Rößler Candy Rowe Hannah M. Rowland Arlety Roy Kaitlin A. Schaal Thomas N. Sherratt John Skelhorn Hannah R. Smart Ted Stankowich Amanda M. Stefan Kyle Summers Christopher H. Taylor Rose Thorogood Kate Umbers Anne E. Winters Justin Yeager Alice Exnerová 《Journal of evolutionary biology》2023,36(7):975-991
Prey seldom rely on a single type of antipredator defence, often using multiple defences to avoid predation. In many cases, selection in different contexts may favour the evolution of multiple defences in a prey. However, a prey may use multiple defences to protect itself during a single predator encounter. Such “defence portfolios” that defend prey against a single instance of predation are distributed across and within successive stages of the predation sequence (encounter, detection, identification, approach (attack), subjugation and consumption). We contend that at present, our understanding of defence portfolio evolution is incomplete, and seen from the fragmentary perspective of specific sensory systems (e.g., visual) or specific types of defences (especially aposematism). In this review, we aim to build a comprehensive framework for conceptualizing the evolution of multiple prey defences, beginning with hypotheses for the evolution of multiple defences in general, and defence portfolios in particular. We then examine idealized models of resource trade-offs and functional interactions between traits, along with evidence supporting them. We find that defence portfolios are constrained by resource allocation to other aspects of life history, as well as functional incompatibilities between different defences. We also find that selection is likely to favour combinations of defences that have synergistic effects on predator behaviour and prey survival. Next, we examine specific aspects of prey ecology, genetics and development, and predator cognition that modify the predictions of current hypotheses or introduce competing hypotheses. We outline schema for gathering data on the distribution of prey defences across species and geography, determining how multiple defences are produced, and testing the proximate mechanisms by which multiple prey defences impact predator behaviour. Adopting these approaches will strengthen our understanding of multiple defensive strategies. 相似文献
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Kunte K Shea C Aardema ML Scriber JM Juenger TE Gilbert LE Kronforst MR 《PLoS genetics》2011,7(9):e1002274
Hybrid speciation, or the formation of a daughter species due to interbreeding between two parental species, is a potentially important means of diversification, because it generates new forms from existing variation. However, factors responsible for the origin and maintenance of hybrid species are largely unknown. Here we show that the North American butterfly Papilio appalachiensis is a hybrid species, with genomic admixture from Papilio glaucus and Papilio canadensis. Papilio appalachiensis has a mosaic phenotype, which is hypothesized to be the result of combining sex-linked traits from P. glaucus and P. canadensis. We show that P. appalachiensis' Z-linked genes associated with a cooler thermal habitat were inherited from P. canadensis, whereas its W-linked mimicry and mitochondrial DNA were inherited from P. glaucus. Furthermore, genome-wide AFLP markers showed nearly equal contributions from each parental species in the origin of P. appalachiensis, indicating that it formed from a burst of hybridization between the parental species, with little subsequent backcrossing. However, analyses of genetic differentiation, clustering, and polymorphism based on molecular data also showed that P. appalachiensis is genetically distinct from both parental species. Population genetic simulations revealed P. appalachiensis to be much younger than the parental species, with unidirectional gene flow from P. glaucus and P. canadensis into P. appalachiensis. Finally, phylogenetic analyses, combined with ancestral state reconstruction, showed that the two traits that define P. appalachiensis' mosaic phenotype, obligatory pupal diapause and mimicry, evolved uniquely in P. canadensis and P. glaucus, respectively, and were then recombined through hybridization to form P. appalachiensis. These results suggest that natural selection and sex-linked traits may have played an important role in the origin and maintenance of P. appalachiensis as a hybrid species. In particular, ecological barriers associated with a steep thermal cline appear to maintain the distinct, mosaic genome of P. appalachiensis despite contact and occasional hybridization with both parental species. 相似文献
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