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Tomoo Kamiya A-Hon Kown Toshiki Kanemaki Yoichi Matsui Shouji Uetsuji Tadayoshi Okumura Yasuo Kamiyama 《In vitro cellular & developmental biology. Animal》1998,34(2):131-137
Summary The Anaeropack system for cell culture, which was originally designed for the growth of anaerobic bacteria, was used to produce
a hypoxic atmosphere for cultured hepatocytes. We measured changes in the oxygen and carbon dioxide concentrations and the
atmospheric temperature in an airtight jar. We also measured changes in the pH of the medium during hypoxia to assess the
accuracy of this system. Moreover, we used three durations (2, 3, and 4 h) of hypoxia and 8 h of reoxygenation in cultured
rat hepatocytes, and then measured the lactate dehydrogenase (LDH), ketone body concentration (acetoacetate + β-hydroxybutyrate),
and the ketone body ratio (KBR: acetoacetate/β-hydroxybutyrate) in the medium in order to assess the suitability of this system
as a model for reperfusion following liver ischemia. The oxygen concentration dropped to 1% or less within 1 h. The concentration
of carbon dioxide rose to about 5% at 30 min after the induction of the hypoxic conditions, and was maintained at this level
for 5 h. No effect of the reaction heat produced by the oxygen absorbent in the jar was recognized. The extent of cell injury
produced by changing the hypoxic parameters was satisfactorily reflected by the KBR, the ketone body concentration, and the
LDH activity released into the medium. Because this model can duplicate the conditions of the hepatocytes during revascularization
following ischemic liver, and the Anaeropack system for cell culture is easy to manipulate, it seems suitable for the experimental
study of hypoxic injury and revascularization in vitro. 相似文献
2.
Yong-ho Lee Mi Hyang Kown Kwang Joon Kim Eun Young Lee Daham Kim Byung-Wan Lee Eun Seok Kang Bong Soo Cha Hyun Chul Lee 《PloS one》2014,9(9)
Background
Glycated albumin (GA) has been increasingly used as a reliable index for short-term glycemic monitoring, and is inversely associated with β-cell function. Because the pathophysiologic nature of type 2 diabetes (T2D) is characterized by progressive decline in insulin secretion, the aim was to determine whether GA levels were affected by diabetes duration in subjects with T2D.Methods
To minimize the effect of glucose variability on GA, subjects with stably maintained HbA1c levels of <0.5% fluctuation across 6 months of measurements were included. Patients with newly diagnosed T2D (n = 1059) and with duration>1 year (n = 781) were recruited and categorized as New-T2D and Old-T2D, respectively. Biochemical, glycemic, and C-peptide parameters were measured.Results
GA levels were significantly elevated in HbA1c-matched Old-T2D subjects compared to New-T2D subjects. Duration of diabetes was positively correlated with GA, whereas a negative relationship was found with C-peptide increment (ΔC-peptide). Among insulin secretory indices, dynamic parameters such as ΔC-peptide were inversely related to GA (r = −0.42, p<0.001). Multiple linear regression analyses showed that duration of diabetes was associated with GA (standardized β coefficient [STDβ] = 0.05, p<0.001), but not with HbA1c (STDβ = 0.04, p<0.095). This association disappeared after additional adjustment with ΔC-peptide (STDβ = 0.02, p = 0.372), suggesting that β-cell function might be a linking factor of close relationship between duration of diabetes and GA values.Conclusions
The present study showed that GA levels were significantly increased in subjects with longer duration T2D and with decreased insulin secretory function. Additional caution should be taken when interpreting GA values to assess glycemic control status in these individuals. 相似文献3.
Protopine is an isoquinoline alkaloid contained in plants in northeast Asia. In this study, we investigated whether protopine derived from Hypecoum erectum L could suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Protopine was found to reduce nitric oxide (NO), cyclooxygenase-2 (COX-2), and prostaglandin E(2) (PGE(2)) production by LPS-stimulated Raw 264.7 cells, without a cytotoxic effect. Pre-treatment of Raw 264.7 cells with protopine reduced the production of pro-inflammatory cytokines. These inhibitory effects were caused by blocking phosphorylation of mitogen-activated protein kinases (MAP kinases) and also blocking activation of a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). 相似文献
4.
Doo Yong Chung Kang Su Cho Dae Hun Lee Jang Hee Han Dong Hyuk Kang Hae Do Jung Jong Kyou Kown Won Sik Ham Young Deuk Choi Joo Yong Lee 《PloS one》2015,10(4)
Purpose
This study was conducted to evaluate colic pain as a prognostic pretreatment factor that can influence ureter stone clearance and to estimate the probability of stone-free status in shock wave lithotripsy (SWL) patients with a ureter stone.Materials and Methods
We retrospectively reviewed the medical records of 1,418 patients who underwent their first SWL between 2005 and 2013. Among these patients, 551 had a ureter stone measuring 4–20 mm and were thus eligible for our analyses. The colic pain as the chief complaint was defined as either subjective flank pain during history taking and physical examination. Propensity-scores for established for colic pain was calculated for each patient using multivariate logistic regression based upon the following covariates: age, maximal stone length (MSL), and mean stone density (MSD). Each factor was evaluated as predictor for stone-free status by Bayesian and non-Bayesian logistic regression model.Results
After propensity-score matching, 217 patients were extracted in each group from the total patient cohort. There were no statistical differences in variables used in propensity- score matching. One-session success and stone-free rate were also higher in the painful group (73.7% and 71.0%, respectively) than in the painless group (63.6% and 60.4%, respectively). In multivariate non-Bayesian and Bayesian logistic regression models, a painful stone, shorter MSL, and lower MSD were significant factors for one-session stone-free status in patients who underwent SWL.Conclusions
Colic pain in patients with ureter calculi was one of the significant predicting factors including MSL and MSD for one-session stone-free status of SWL. 相似文献5.
Effects of crude oil on marine microbial communities in short term outdoor microcosms 总被引:1,自引:0,他引:1
Jung SW Park JS Kown OY Kang JH Shim WJ Kim YO 《Journal of microbiology (Seoul, Korea)》2010,48(5):594-600
To assess the effects of crude oil spills on marine microbial communities, 10 L outdoor microcosms were manipulated over an
exposure period of 8 days. The responses of microbial organisms exposed to five crude oil concentrations in 10 to 10,000 ppm
(v/v) were monitored in the microcosms. The abundance of microalgae and copepods decreased rapidly upon the addition of crude
oil at concentrations over 1,000 ppm, whereas the total density of heterotrophic bacteria increased dramatically at the higher
concentrations. Bacterial diversity, determined by denaturing gradient gel electrophoresis, was increased at higher concentrations.
In particular, the intensity of the bands representing Jannaschia sp. and Sulfitobacter brevis increased with the addition of oil. These results indicate that crude oil spills with concentrations over 1,000 ppm seriously
affected the structure of the microbial communities. 相似文献
6.
Targeted toxins represent a new approach to specific cytocidal therapy. Immunotoxins based on plant and microbial toxins are very immunogenic. To develop a targeted therapy that is less immunogenic and easily invades target tissues, four fusion proteins containing human angiogenin targeted by human EGF have been constructed. EGF is a single chain polypeptide, which binds to epidermal growth factor receptor (EGFR) and is known to be internalized by endocytosis. Angiogenin has been separately fused either at the amino terminus or the carboxyl terminus of EGF via linkers, giving rise to angiogenin-gly-EGF, angiogenin-(gly)4ser-EGF and EGF-angiogenin, EGF-gly-angiogenin, respectively. The fusion proteins were over-expressed in Escherichia coli and purified from periplasmic eluents by affinity chromatography. EGF-angiogenin and EGF-gly-angiogenin maintained receptor-binding activity of EGF and RNase activity of angiogenin in a single peptide and actively inhibited growth of human EGFR-positive target cells in culture. They are expected to have a very low immunogenic potential in humans because of their endogenous origin and also to have another potential therapeutic advantage because these fusion proteins may have overcome conventional immunotoxin and possess increased ability to penetrate because of their small size. 相似文献
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