排序方式: 共有4条查询结果,搜索用时 15 毫秒
1
1.
Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis) 总被引:2,自引:0,他引:2
A tissue-specific cDNA library was constructed using polyA+ RNA from pituitary glands of the Indian catfishHeteropneustes fossilis (Bloch) and a cDNA clone encoding growth hormone (GH) was isolated. Using polymerase chain reaction (PCR) primers representing
the conserved regions of fish GH sequences the 3′ region of catfish GH cDNA (540 bp) was cloned by random amplification of
cDNA ends and the clone was used as a probe to isolate recombinant phages carrying the full-length cDNA sequence. The full-length
cDNA clone is 1132 bp in length, coding for an open reading frame (ORF) of 603 bp; the reading frame encodes a putative polypeptide
of 200 amino acids including the signal sequence of 22 amino acids. The 5′ and 3′ untranslated regions of the cDNA are 58
bp and 456 bp long, respectively. The predicted amino acid sequence ofH. fossils GH shared 98% homology with other catfishes. Mature GH protein was efficiently expressed in bacterial and zebrafish systems
using appropriate expression vectors. The successful expression of the cloned GH cDNA of catfish confirms the functional viability
of the clone. 相似文献
2.
3.
R -M. Pitchappan V -N. Kakkaniah M. Manickasundari V. Rajaram A. Koteeswaran 《Journal of genetics》1989,68(2):75-92
Eighty-three patients with psoriasis vulgaris, living in Madurai, Tamil Nadu, India, were studied for HLA-A, HLA-B, HLA-C,
HLA-DR and HLA-DQ antigen frequencies and compared with seventy-seven controls studied using the same batch of reagents. A
highly significant increase of frequency of HLA-Bw57, a split of HLA-B 17, was found in the patients; Bw58, another split
of B17, was absent. Relative risk was high for A1, B17, Bw57 and DR7 individuals; it was highest for Bw57. Frequencies of
the haplotypesAl-Bw57 andDR7-DQw3 were also significantly higher in patients. Analysis of the HLA data based on ethnic differences identified as major groups
revealed high relative risk for B17, Bw57 and DR7 only in major group III, a Western brachycephal Armenoid group, but not
in major group II, a Mediterranean one thought to be an earlier settler of this region. Analysis of the data based on age
and sex subgroups yielded interesting information. The age at onset of the disease in the total patient sample showed a bimodal
distribution. The two sexes differed in their age-at-onset distributions: females showed a preponderance of early onset of
the disease (< 30 years of age, 68%), while the majority of males had late onset (>30 years of age, 71%). HLA data for the
early-onset patients indicate very high relative risk for B17, Bw57 and DR7. This suggests that psoriasis may be influenced
by sex, and that the early-onset and late-onset forms of the disease may be of different aetiopathogenesis. These observations
stress the importance of considering the ethnic origin or composition of samples, and age, sex and other parameters in HLA
and disease association studies. 相似文献
4.
Ploidy induction and sex control in fish 总被引:19,自引:0,他引:19
1