The effects of methyl jasmonate and salicylic acid on the production of secondary metabolites in organ culture of Ziziphora persica

Plant Cell, Tissue and Organ Culture (PCTOC) - Ziziphora persica (Lamiaceae) has been used as infusions and decoctions in traditional medicine for various purposes such as sedative, carminative,...     

Toxicity impacts of chemically and biologically synthesized CuO nanoparticles on cell suspension cultures of <Emphasis Type="Italic">Nicotiana tabacum</Emphasis>

Nanotechnology has quite a lot of applications in various fields of industrial sectors like food and agriculture. Although nanotechnology can improve the quality of life, its possible associated risks should be assessed. Here copper oxide nanoparticles (CuO NPs) were synthesized by chemical (polymer pyrolysis) and biological (green) methods with an average size of 30 and 44 nm, respectively. Afterwards, a cell biology approach was applied to evaluate the toxic effects of chemically and biologically synthesized CuO nanoparticles on tobacco cell suspension cultures. Both types of CuO nanoparticles significantly dropped the viability of the cells in a dose and time dependent manner. Accordingly, tobacco cells were found to increase the activity of antioxidant enzymes after 48 h of exposure to nanoparticles. The production of reactive oxygen species (ROS) and malondialdehyde (MDA) in a dose dependent manner was also observed. Assessment of the toxicity of CuO NPs revealed that chemically synthesized NPs were more toxic than biologically synthesized ones. It can be concluded that the organic components of the plant extract as capping agents that remain on the surface of green synthesized CuO NPs may reduce their toxicity effects.