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The effect of lincocin (a plastid protein synthesis inhibitor) treatment on the greening process of bean (Phaseolus vulgaris L.) leaves have been studied. In comparison with control leaves treated ones had a decreased rate of chloroplast development. They had a marked chlorophyll deficiency and a decreased chlorophyll a/b ratio. Some long and short wavelength forms of chlorophyll a were lacking as evidenced from the absorption spectra at 25°C and the fluorescence spectra at 77°K. The –14CO2 fixation was inhibited by 80–90% in treated leaves. The fluorescence induced by the measuring light was greater in the treated leaves than in the control ones, and the kinetics of the decline of the relative fluorescence intensity were also different. Electron microscopic studies showed macrogranum-like structures and incomplete membrane vesicles in the treated plastids. After longer treatment a destruction of membranes was observed. The results indicate some structural and functional membrane deficiencies and instability of the membranes. 相似文献
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Brylev V. A. Lysenko I. L. Kokin E. A. Martynenko-Makaev Y. V. Ryazantsev D. Y. Shmanai V. V. Korshun V. A. 《Russian Journal of Bioorganic Chemistry》2021,47(3):734-740
Russian Journal of Bioorganic Chemistry - An azido-derivative of a fluorescein bifluorophore was obtained and used for the synthesis of “molecular beacon”-type oligonucleotide... 相似文献
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Maksim V. Kvach Igor A. Prokhorenko Alexey V. Ustinov Sergei V. Gontarev Vladimir A. Korshun Vadim V. Shmanai 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):809-813
New reagents (CPGs and phosphoramidites) for automatic solid phase synthesis of modified oligonucleotides were designed. Three oligonucleotides carrying fluorescent label at the 5′-terminus and an anchor group at the 3′-terminus were prepared and their immobilization in orthogonal conditions on solid supports was studied. 相似文献
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DIDIER FOURGON IGOR EECKHAUT DEVARAJEN VAÏTILINGON MICHEL JANGOUX 《Invertebrate reproduction & development.》2013,57(3):155-165
Summary The larval development of the ophiocomid ophiuroid Ophiomastix venosais described using SEM. The gastrula transforms into a uniformly ciliated early larva which progressively changes into a lecithotrophic late premetamorphic larva with a continuous bilateral ciliated band. This stage is short-lived and equivalent to a highly reduced ophiopluteus. Comparisons between O. venosa and other ophiuroid species whose development has been investigated suggest that, whatever the developmental mode (lecithotrophic or planktotrophic), a pluteus stage always occurs in ophiuroids with planktonic development. Two metamorphic stages were identified, the late metamorphic larva differing from the early one by the closure of the larval mouth. The appearance of the permanent mouth marks the end of the metamorphosis. The postlarva still possesses remnants of larval features. The transformation of the reduced ophiopluteus into a barrel-shaped metamorphic larva with transverse ciliated bands, a vitellaria larva, is followed. The possible occurrence of a unique type of metamorphic larva in non-brooding ophiuroids is discussed. Verification of this, however, needs further SEM investigations on metamorphic larva from species having “regular” planktotrophic development. 相似文献
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Recent data are reviewed on the employment of fluorescence resonance energy transfer (FRET) in studying hybridization and higher structures of nucleic acids as well as their enzyme- and ribozyme-catalyzed reactions. 相似文献
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Skorobogatyj MV Pchelintseva AA Ustinov AV Korshun VA Malakhov AD 《Nucleosides, nucleotides & nucleic acids》2005,24(5-7):931-934
Two fluorescent nucleosides, 5-(perylen-3-ylethynyl)-2'-deoxyuridine and 5-[(perylen-3-yl)methoxypropyn-1-yl]-2'-deoxyuridine, were incorporated into synthetic oligodeoxyribonucleotides and spectral properties of the conjugates and their duplexes were studied. 相似文献
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V. A. Korshun I. A. Prokhorenko S. V. Gontarev M. V. Skorobogatyi K. V. Balakin E. V. Manasova 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1461-1464
Abstract A series of pyrene-containing reagents have been synthesized and used for the fluorescent labeling of oligonucleotides. 相似文献
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A. D. Malakhov E. V. Malakhova S. V. Kuznitsova I. V. Grechishnikova I. A. Prokhorenko M. V. Skorobogatyi V. A. Korshun Yu. A. Berlin 《Russian Journal of Bioorganic Chemistry》2000,26(1):34-44
Novel reagents for the fluorescent labeling of oligo- and polynucleotides have been prepared: 5-(1-pyrenylethynyl)-2′-deoxyuridine
3′-phosphoramidite and a solid support carrying this nucleoside. Oligo-nucleotides containing one or several modified units
have been synthesized, and the fluorescence of these probes has been shown to change upon hybridization with the complementary
sequence.
Fluorescent Nucleosides. III. The previous communications, see [1, 2]. Prefix “d” in the oligodeoxynucleotide designations
is omitted. 相似文献