排序方式: 共有19条查询结果,搜索用时 15 毫秒
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M R Kopantseva M Z Liudvig I I Uspenski? N A Tamarina V A Tsatrian L I Korochkin 《Zhurnal obshche? biologii》1990,51(1):125-140
A comparative electrophoretic study or ejaculatory bulb proteins in 29 different Drosophila species has been carried out. In all analyzed species, ejaculatory bulb contains a major component (designated as PEB). It has molecular mass of 61-65 kDa in the species of virilis group, 33-36 kDa in species of obscura group, and 34-56 kDa in species of melanogaster group. Using immunoblotting technique, we have demonstrated that PEB is introduced into organs of female sex tract during mating. The nature and significance of revealed interspecific differences in PEB proteins has been discussed. 相似文献
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Michael Z. Ludwig Ilya I. Uspensky Andrew I. Ivanov Maria R. Kopantseva Christem M. Dianov Natalia A. Tamarina Leonid I. Korochkin 《Biochemical genetics》1991,29(5-6):215-239
PEB-me is a predominant protein of matureDrosophila melanogaster ejaculatory bulbs. It is resolved into four or five closely spaced subfractions (apparent molecular weight 35–39 kD) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Four electrophoretic variants of PEB-me differing in apparent molecular weight by 200–800 daltons were found. These appear to be controlled by four alleles of a gene (peb) located by recombination and deletion mapping to the 60F1-2 region of chromosome 2. A minor ejaculatory bulb protein of ca. 80 kD (hPEB) was found to be immunochemically related to PEB and possibly encoded bypeb. PEB is not detected by immunoblotting techniques in virgin females, in male tissues other than the ejaculatory bulb, or during developmental stages preceding the formation of this organ. The results of transplantations of genital imaginal discs and of immature ejaculatory bulbs between two strains having different PEB alleles suggest that the ejaculatory bulb is the site of PEB synthesis. In flies mutant fortra, tra-2, dsx, orix, tissue specificity of PEB localization is retained and the protein is found whenever the ejaculatory bulb is formed, regardless of the chromosomal sex of the fly. The protein is transferred into the female genital duct during mating, where it can be detected for up to 12 hr. Possible functions of PEB inDrosophila reproduction are discussed. 相似文献
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Kandarakov O. F. Demin A. M. Popenko V. I. Leonova O. G. Kopantseva E. E. Krasnov V. P. Belyavsky A. V. 《Molecular Biology》2020,54(1):99-110
Molecular Biology - The factors that affect the labeling of NIH 3T3 murine fibroblasts with Fe3O4-based magnetic nanoparticles (MNPs) were studied using MNPs produced by the gas condensation and... 相似文献
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L. Zhang N. G. Gurskaya Y. E. Kopantseva N. N. Mudrik L. L. Vagner K. A. Lukyanov D. M. Chudakov 《Russian Journal of Bioorganic Chemistry》2010,36(2):179-184
The site-directed mutagenesis of the monomeric red fluorescent protein TagRFP and its variants was performed with the goal
of generating reversibly photoactivatable fluorescent proteins. Amino acids at positions 69, 148, 165, 179, and 181 (enumeration
according to the green fluorescent protein GFP) were shown to play a key role in the manifestation of the photoactivatable
properties. A reversibly photoactivatable red fluorescent protein KFP-HC with excitation and emission maxima at 585 and 615
nm, respectively, was generated. The KFP-HC fluorescent intensity was decreased by 5–10 times under green light (530–560 nm)
irradiation (due to the fall of the fluorescence quantum yield) and restored under irradiation with blue light (450–490 nm)
or after incubation in the dark (recovery half-time of 30 min). 相似文献
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Kiriukhin DO Shustikova LA Kopantseva EE Luchinskaia NN Beliavskiĭ AV 《Biofizika》2010,55(6):1002-1007
The development of multicellular organisms is a complicated coordinated process of the movement of groups of embryonic cells, which is controlled by many regulatory systems. At present little is known about the regulation of the earliest manifestations of the movement in the embryogenesis: epiboly and radial intercalation. The coordinators of these processes may be small GTPases of the Rho family and their activators, the factors of exchange of guanylic nucleotides. It has been shown in this work that the overexpression of the factor of exchange of guanylic nucleotides xLARG in Xenopus laevis embryos leads to an increase in the amount of the active form of xLARG. In addition, an increase in the expression of xLARG disturbs the process of radial intercalation. The data obtained suggest that xLARG is involved in maintaining the xLARG activation level necessary for the occurrence of epiboly. 相似文献
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