全文获取类型
收费全文 | 352篇 |
免费 | 64篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 10篇 |
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 3篇 |
2015年 | 8篇 |
2014年 | 10篇 |
2013年 | 9篇 |
2012年 | 18篇 |
2011年 | 15篇 |
2010年 | 9篇 |
2009年 | 14篇 |
2008年 | 22篇 |
2007年 | 16篇 |
2006年 | 23篇 |
2005年 | 20篇 |
2004年 | 21篇 |
2003年 | 22篇 |
2002年 | 26篇 |
2001年 | 30篇 |
2000年 | 27篇 |
1999年 | 21篇 |
1998年 | 6篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1994年 | 12篇 |
1993年 | 11篇 |
1992年 | 9篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 10篇 |
1988年 | 4篇 |
1984年 | 2篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1960年 | 1篇 |
排序方式: 共有416条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
5.
Unexpected sequence similarity between nucleosidases and phosphoribosyltransferases of different specificity. 总被引:1,自引:0,他引:1 下载免费PDF全文
A. R. Mushegian E. V. Koonin 《Protein science : a publication of the Protein Society》1994,3(7):1081-1088
Amino acid sequences of enzymes that catalyze hydrolysis or phosphorolysis of the N-glycosidic bond in nucleosides and nucleotides (nucleosidases and phosphoribosyltransferases) were explored using computer methods for database similarity search and multiple alignment. Two new families, each including bacterial and eukaryotic enzymes, were identified. Family I consists of Escherichia coli AMP hydrolase (Amn), uridine phosphorylase (Udp), purine phosphorylase (DeoD), uncharacterized proteins from E. coli and Bacteroides uniformis, and, unexpectedly, a group of plant stress-inducible proteins. It is hypothesized that these plant proteins have evolved from nucleosidases and may possess nucleosidase activity. The proteins in this new family contain 3 conserved motifs, one of which was found also in eukaryotic purine nucleosidases, where it corresponds to the nucleoside-binding site. Family II is comprised of bacterial and eukaryotic thymidine phosphorylases and anthranilate phosphoribosyltransferases, the relationship between which has not been suspected previously. Based on the known tertiary structure of E. coli thymidine phosphorylase, structural interpretation was given to the sequence conservation in this family. The highest conservation is observed in the N-terminal alpha-helical domain, whose exact function is not known. Parts of the conserved active site of thymidine phosphorylases and anthranilate phosphoribosyltransferases were delineated. A motif in the putative phosphate-binding site is conserved in family II and in other phosphoribosyltransferases. Our analysis suggests that certain enzymes of very similar specificity, e.g., uridine and thymidine phosphorylases, could have evolved independently. In contrast, enzymes catalyzing such different reactions as AMP hydrolysis and uridine phosphorolysis or thymidine phosphorolysis and phosphoribosyl anthranilate synthesis are likely to have evolved from common ancestors. 相似文献
6.
7.
8.
9.
The availability of complete genome sequences of cellular life forms creates the opportunity to explore the functional content of the genomes and evolutionary relationships between them at a new qualitative level. With the advent of these sequences, the construction of a minimal gene set sufficient for sustaining cellular life and reconstruction of the genome of the last common ancestor of bacteria, eukaryotes, and archaea become realistic, albeit challenging, research projects. A version of the minimal gene set for modern-type cellular life derived by comparative analysis of two bacterial genomes, those of Haemophilus influenzae and Mycoplasma genitalium, consists of ∼250 genes. A comparison of the protein sequences encoded in these genes with those of the proteins encoded in the complete yeast genome suggests that the last common ancestor of all extant life might have had an RNA genome. 相似文献
10.