Effects of applying oxytocin to Helix pomatia neurons. I. Depolarization

Inward current produced by applying oxytocin (OT) to the neuronal soma (OT_I) current) under conditions of voltage-clamping at the cell membrane was investigated inHelix pomatia. Replacing sodium with Tris ions in the external medium produced a considerable decline in OT_I current. A reduction in the external concentration of chlorine ions by replacement with HEPES ions induced an increase in OT_I current and a shift in its current-voltage relationship towards depolarization values. The presence of furosemide in the external solution reversibly inhibited OT_I current. This current likewise declined reversibly following external application of imidazole and tolbutamide but was increased by theophylline action. It was inferred that OT receptors are present on the surface membrane of someHelix neurons which, when activated, lead to increased chlorine permeability — a process apparently mediated via the cyclic nucleotide system.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR. Kiev. Translated from Neirofiziologiya, Vol. 20, No. 5, pp. 652–659, September–October, 1988.