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Some novel transcription attenuation mechanisms used by bacteria 总被引:2,自引:0,他引:2
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Matić Ivana Z. Ergün Sercan Đorđić Crnogorac Marija Misir Sema Aliyazicioğlu Yüksel Damjanović Ana Džudžević-Čančar Hurija Stanojković Tatjana Konanç Kalbiye Petrović Nina 《Cytotechnology》2021,73(3):373-389
Cytotechnology - Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity... 相似文献
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Konan Gervais N’Zi Stanislas Silvain Yao Gouli Gooré Bi Valentin Ndouba 《Saudi Journal of Biological Sciences》2015,22(3):265-273
The general aim of this study is to update the inventory of the fish species and to specify distribution patterns in the Nero River ichthyofauna in order to establish some basis for the conservation of these fish communities and their habitat. From February 2009 to January 2010, thirty-three sites were sampled monthly with gill nets and a backpack electrofisher, and environmental variables were recorded. Overall, 46 species included in 33 genuses, 24 families and 9 orders were collected. Eleven families and 30 species were the first records for the Nero River. Including all species previously listed in the literature, the number of species presently known in the Nero River and its tributaries is revised to 59. Four families, Alestidae (21%), Schilbeidae (19%), Cyprinidae (17%) and Cichlidae (16%) that made up 73% of the total number of the catches, were the most dominant. The most dominant numerical species were Schilbe mandibularis and Brycinus longipinnis. Fish species and sampling sites along with eight environmental variables were ordinated with canonical correspondence analysis (CCA) coupled to the Monte Carlo test. Ecological status based on fish assemblage according to environmental variables and anthropogenic pressures showed that miss dead wood leaves and roots, electrical conductivity, total dissolved solids, mud, nitrite, basin width, dissolved oxygen and pH, were the primary factors influencing fish distribution. The environmental tolerance index (ETI), ecological tolerance (tk) and optima (uk) values of 10 species to 8 different environmental variables were analyzed. Six species (Hemichromis fasciatus, Epiplatys chaperi, Barbus ablabes, B. longipinnis, Hemichromis bimaculatus and Chromidotilapia guntheri) have high ETI and a cosmopolitan distribution in the Nero River. In the tributaries of the middle course, high concentrations of nitrite in the water, added to the presence of a lot of tolerant species in the ichthyofauna are indications of disturbance of these areas. Subsequent recommendations were formulated for efficient restoration and conservation management of this River. 相似文献
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Felicia N. Anike Koffi Konan Kouadio Olivier Hortense Dodo 《Plant Cell, Tissue and Organ Culture》2012,111(3):303-313
Shoot organogenesis was successfully achieved in petiole explants excised from 6 to 8-week old in vitro plantlets of yam Dioscorea rotundata P. cv. Kponan fissa, Dioscorea cayenensis L. cv. Krengle IB14 and cv. Krengle IB35, and Dioscorea alata L. cv. Bete bete. Only the basipetal portion of the petiole acquired competence, and plants regenerated within 21?days on MS medium supplemented with 2?% sucrose, 100?mg/l myo-inositol, 10???M kinetin and 1.5?mM putrescine referred to as yam regeneration medium (YRM). Plant regeneration was significantly (p?<?0.01) higher (42?%) with 2?% sucrose compared to 1, 3 and 4?% sucrose which produced 4, 25 and 15?% regeneration respectively. The age of the donor yam plantlet was critical to regeneration, and the highest regeneration efficiency was obtained with explants from 8-week old plantlets. Addition of the antioxidants lipoic acid (19.4???M), and l-cysteine (28.5???M) to the culture medium stimulated axillary branching in regenerated shoots. Among the four yam cultivars tested, cv. Kponan fissa, cv. Krengle IB14 and cv. Bete bete had similar response at 10???M kinetin, while the cv. Krengle IB35 regenerated best at a lower concentration of kinetin (0.5???M). 相似文献
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400-MHz 1H-NMR spectroscopy of fucosylated tetrasialyl oligosaccharides isolated from normal and cirrhotic alpha 1-acid glycoprotein 总被引:2,自引:0,他引:2
The comparative study of fucosylated tetrasialyl-oligosaccharides isolated from normal and cirrhotic alpha 1-AGP was performed using permethylation and 400-MHz 1H-NMR spectroscopy. These results clearly show the tetraantennary structure of these two oligosaccharides with hyperfucosylation for the tetrasialylated fraction from cirrhotic alpha 1-AGP. In the latter oligosaccharide the simultaneous presence on two antennae (7 and 7') of the sialosyl Lewis X determinant NeuAc-(alpha 2-3) Gal(beta 1-4) [Fuc(alpha 1-3)] GlcNAc has been observed. Moreover the 5 and 5' antennae were alpha 2-6 sialylated but without fucose. 相似文献
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Cartridge-based high-throughput purification of oligonucleotides for reliable oligonucleotide arrays 总被引:1,自引:0,他引:1
Semenyuk A Ahnfelt M Estmer Nilsson C Yong Hao X Földesi A Kao YS Chen HH Kao WC Peck K Kwiatkowski M 《Analytical biochemistry》2006,356(1):132-141
A novel, cartridge-based procedure for the efficient and irreversible detritylation of oligonucleotides is reported. This method, combined with a process for the elimination of depurinated fragments produces, in a highly parallel fashion, oligonucleotides with better purity than those traditionally obtained using reversed-phase high-performance liquid chromotography purification. Our combined detritylation and purification methodology compares favorably with commercial cartridge-based purification systems. The benefits of working with pure oligonucleotides, with regard to higher signal and better signal linearity, are shown in array-based hybridization experiments. 相似文献