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1.
The sperm cells ofPatella coerulea (Patellacea),Monodonta turbinata, andGibbula tumida (Trochacea) were investigated by means of transmission electron microscopy. They belong to the primitive type (sensu Franzén) and have more features in common with primitive Bivalvia sperms than with Neritacea. Their head contains an apical acrosome and a roundish nucleus followed by 4 or 5 mitochondria and a centriolar apparatus which consists of two centrioles, one of which bears a flagellum. The sperm cells ofMonodonta andGibbula are very similar to each other and differ mainly in size;Patella exhibits more differences (very small acrosome, subacrosomal space, variable number of spherical mitochondria (origin of spermic dimorphism ?). The development of the sperm cells shows no peculiarities. 相似文献
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Kinetic studies on hepatic handling of glucagon using the model of non-recirculating perfused rat livers. 总被引:1,自引:0,他引:1
Using the model of the in vitro non-recirculating perfused rat liver we studied kinetic aspects of the hepatic handling of glucagon. Under conditions of a 20 min glucagon infusion (glucagon mass flows of 0.05, 0.46 and 4.75 ng/g liver/min, respectively) according to a rectangular profile both total and individual glucagon extractions were dependent on mass flow and time. The time course of glucagon extraction started with an acute phase within the first minute of infusion with a maximum value of 70%, which decreased within the following 30 sec by more than 40%. Depending on concentration, there was a progressive decrease in the hepatic extraction of glucagon up to the end of perfusion. Hepatic glucagon degradation was found to take place only at a little extent. Immediately after terminating the hormone infusion, the liver changed over into a glucagon-releasing organ. Kinetics of glucagon infusion and glucagon-induced hepatic glycogenolysis did not distinguish by parallelism but rather by phase shifting. 相似文献
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T A Rapoport K D Kohnert S Knospe A Lukowsky S Prehn H Sch?fer S Schmidt M Ziegler 《Acta biologica et medica Germanica》1978,37(8):1153-1160
Insulin of carp (Cyprinus carpio) was isolated and crystallized. The insulin was biologically active in two tests; it decreased the blood glucose level and stimulated 14CO2-formation from glucose. The chemical properties are similar to those of insulins from other species. The insulins of carp and of mammals differ greatly immunologically. Antibodies against carp insulin crossreact with carp proinsulin. 相似文献
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K.-D. Kohnert H.-J. Hahn H. Zühlke S. Schmidt H. Fiedler 《Biochimica et Biophysica Acta (BBA)/General Subjects》1974,338(1):68-77
Pancreatic islets were isolated from Wistar rats, albino mice, spiny mice and sand rats (Psammomys obesus). Evidence is presented that pancreatic islets contain an enzyme system degrading insulin in the presence of glutathione or other sulfhydryl-containing compounds. Apparent Km values for insulin and glutathione (in the presence of EDTA) are 14.0 μM (mol. wt 5700) and 1.28 mM, respectively. Maximum breakdown of 125I-labeled insulin was found at about pH 7.2. After ultracentrifugation of islet homogenates the microsomal fraction contained the greatest relative specific insulin-degrading activity. The specific insulin-degrading actvitity was found to be higher in Wistar rats and albino mice than in spiny mice and sand rats. Starvation of Wistar rats for 72 h caused a decrease inthe enzymatic activity. 相似文献
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Pancreatic islets of Wistar rats were prepared by digestion with collagenase and then washed and isolated at three different temperatures (4, 22 and 37 degrees C). The efficiency of washing with regard to proteolytic and collagenolytic activities in the wash buffer was not affected by the temperatures used. The islet thiol:protein-disulphide oxidoreductase activity (EC 1.8.4.2) was apparently unchanged, whereas washing temperatures lower than 37 degrees C resulted in a diminished insulin content. The insulin secretion of islets, isolated at 4 degrees C, is reduced in response to glucose without changing the sigmoidal shape of dose-response curve. 相似文献
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M Ziegler S Teneberg S Witt B Ziegler B Hehmke K D Kohnert J Egeberg K A Karlsson A Lernmark 《Journal of immunology (Baltimore, Md. : 1950)》1988,140(12):4144-4150
Diabetes was induced in BALB/c mice by four injections of a subdiabetogenic dose (40 mg/kg) of streptozotocin in combination with CFA. The treatment increased the plasma glucose from 5.8 +/- 0.1 to 22.1 +/- 1.3 mmol/liter (n = 9). The diabetic animals had circulating islet cell surface antibodies (75%), and a monoclonal islet cell surface IgM antibody, K56aF3, generated from one of the diabetic BALB/c mice, mediated C-Dependent cytotoxicity against insulin-producing cells and inhibited glucose-stimulated insulin release from isolated rat islets. Solid phase assay on thin layer chromatograms showed no binding of the K56aF3 antibody to glycolipids prepared from relevant cells. However, testing against a series of glycolipids of various non-pancreatic origins showed a preferential binding to a nine-sugar glycolipid isolated from human erythrocytes carrying an unusual blood group A determinant (type 3). It is suggested that this mAb may be associated with the development of diabetes following a combination of polyclonal activation and non-diabetogenic doses of streptozotocin. 相似文献
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Mapping of the catalytic site of CHO-t-PA and the t-PA variant BM 06.022 by synthetic inhibitors and substrates.
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J. Stürzebecher U. Neumann U. Kohnert G. B. Kresse S. Fischer 《Protein science : a publication of the Protein Society》1992,1(8):1007-1013
BM 06.022 is a t-PA deletion variant that is produced as inactive inclusion bodies in Escherichia coli and transformed into the native form by an in vitro refolding process. Until now, no X-ray and NMR structures of BM 06.022 were available. Therefore a detailed kinetic analysis of the hydrolysis of peptide substrates and of the inhibition by several benzamidine-derived inhibitors was carried out in order to assess that the active site region of the protease domain of BM 06.022 is correctly structured in comparison with t-PA. Our data reveal that the single-chain as well as the two-chain form of BM 06.022 and native t-PA are similar in catalytic and in inhibitor binding properties. This indicates that the active site and the highly complex rearrangement of t-PA upon cleavage of the Arg275-Ile276 bond are maintained in BM 06.022. 相似文献
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