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Summary A growth associated formation of extracellular 5-aminolevulinic acid (ALA) was found in the homoacetogenesis of glucose byClostridium
thermoaceticum grown in minimal defined medium. The growth and ALA production was enhanced by L-cysteine HCl both in complex medium (UM) and minimal defined medium (MDM). The amount of ALA produced extracellularly in MDM wasca. 15 mg/L after 90-h anaerobic cultivation (cell-mass: 1.5 g/l; glucose consumed: 20 g/l). 相似文献
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Sato Akira Watanabe Yoshio Nugroho Nuki Bambang Chrisnayanti Evita Natusion Uli Julia Koesnandar Nishida Hiroshi 《World journal of microbiology & biotechnology》2003,19(7):763-766
A total of 500 strains of basidiomycetes isolated from temperate forests in Japan and 379 strains from tropical forests in Indonesia were subjected to a laboratory screening for dioxin-degrading ability. At first, about 200 fungal strains were selected by their ability to decolorize Remazol Brilliant Blue R dye as an indicator of ligninolytic activities. Next, for excluding the factor of dioxin sorption by mycelia, we prepared two series of living cultures exposed either long-term or short-term to 2,7-dichlorodibenzo-p-dioxin (2,7-DCDD), and compared the decreases in the remaining amounts of this model compound. We chose Bjerkandera adusta strain VH57 as a promising new candidate for dioxin degradation, because it gave 40% difference in 2,7-DCDD levels between the two treatments after 30 days of exposure. 相似文献
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Ingrid S. Surono Fiona P. Koestomo Nuni Novitasari Fransiska R. Zakaria Yulianasari Koesnandar 《Anaerobe》2011,17(6):496-500
Enterococcus faecium IS-27526 is a novel probiotic isolated from dadih, an Indonesian traditional fermented buffalo milk. A 90 days randomized double-blind placebo-controlled study ofpre-post trial was conducted in pre-school children with two groups, placebo and probiotic group. Ultra High Temperature low fat milk was used as a carrier in each group. The aims of this study were to investigate the effect of E. faecium IS-27526 in milk on humoral immune response and on bodyweight of pre-school children. Total serum IgA and total salivary sIgA were measured by sandwich ELISA. The bodyweight of young children was measured. The results showed that total serum IgA did not significantly increase in the probiotic group compared with the placebo group. Total salivary sIgA level and the bodyweight significantly increased (p < 0.05) in probiotic groups compared to the placebo. Changes of total salivary sIgA level were significantly higher in underweight children supplemented with probiotic. Weight gain was observed significantly in children with normal bodyweight supplemented with probiotic. Neither mortality nor weight loss was recorded throughout the study. Taken together, novel probiotic E. faecium IS-27526 has significant positive effects on humoral immune response, salivary sIgA, in underweight pre-school children, and on weight gain of pre-school children. 相似文献
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Aneth Koesnandar Naomichi Nishio Shiro Nagai 《Applied microbiology and biotechnology》1990,32(6):711-714
Summary An acetate-tolerant strain of Clostridium thermoaceticum (ATCC 31490), characterized as growing on a tryptone-yeast extract complex medium, was adapted to grow in a minimal medium containing glucose as sole carbon source. After five sequential batch cultures involving reducing and deleting organic materials from the original complex medium, the bacterium could grow on minimal medium with a supplement of nicotinic acid. However, growth and homoacetogenesis from glucose was remarkably retarded compared with that observed in the original complex medium. This could be strikingly improved up to the original level by replacing sodium thioglycolate (a reducing agent in the original medium) with L-cysteinHCI supplemented to the minimal medium. Cysteine played an important role in stimulating growth and homoacetogenesis by not only supplying H2S as a sulphur source via cysteine desulphydrase (EC 4.4.1.1) but also maintaining a proper redox potential (—370 and —400 mV) in the growth phase during culture. Methionine could not serve as a sulphur source.Offprint requests to: Shiro Nagai 相似文献
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