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排序方式: 共有76条查询结果,搜索用时 703 毫秒
1.
Congenital chromosome breakage clusters within Giemsa-light bands and identifies sites of chromatin instability 总被引:1,自引:0,他引:1
Analysis of the distribution of published chromosome breaks in cells with constitutional chromosome aberrations showed a nonrandom distribution of breaks among chromosomes and chromosome regions. A significant amount of breakage occurred at Giemsa-negative bands. In addition, chromosome sites associated with a number of fragile sites and cellular oncogene sites were affected nonrandomly. The data are consistent with the hypothesis that chromosome breakage occurs in somatic or germ cells as a result of recombinational errors involving actively transcribing chromatin regions or regions of unstable DNA sequence structure placed in proximity during interphase. 相似文献
2.
This study was conducted to examine the psycho-emotional effects of repeated oral exposure to capsaicin, the principal active component of chili peppers. Each rat received 1 mL of 0.02% capsaicin into its oral cavity daily, and was subjected to behavioural tests following 10 daily administrations of capsaicin. Stereotypy counts and rostral grooming were significantly increased, and caudal grooming decreased, in capsaicin-treated rats during the ambulatory activity test. In elevated plus maze test, not only the time spent in open arms but also the percent arm entry into open arms was reduced in capsaicin-treated rats compared with control rats. In forced swim test, although swimming duration was decreased, struggling increased in the capsaicin group, immobility duration did not differ between the groups. Repeated oral capsaicin did not affect the basal levels of plasma corticosterone; however, the stress-induced elevation of plasma corticosterone was prolonged in capsaicin treated rats. Oral capsaicin exposure significantly increased c-Fos expression not only in the nucleus tractus of solitarius but also in the paraventricular nucleus. Results suggest that repeated oral exposure to capsaicin increases anxiety-like behaviours in rats, and dysfunction of the hypothalamic-pituitary-adrenal axis may play a role in its pathophysiology. 相似文献
3.
Murali Mohan Challa Santosh Kumar Sanivada Uma Devi Koduru 《Biocontrol Science and Technology》2013,23(10):1169-1185
Intracellular total soluble proteins of Beauveria bassiana are believed to play an important role in virulence against insect hosts. Thirty B. bassiana isolates collected from different geographical regions and host ranges were characterised by total soluble proteins present in cells, using the SDS–PAGE technique to differentiate the isolates based on virulence and host insect origin. In vitro analysis of total soluble protein profiles of 30 isolates was studied to understand the relationship of isolates with their host of origin and virulence against Helicoverpa armigera. There was a positive relationship between virulence and host origin. All the non-virulent isolates are grouped together. Similarly, highly virulent isolates against H. armigera were grouped together. The relationship between total soluble proteins and pathogenicity was positively correlated. Thirty isolates shared only 22% similarity in their protein profiles. 相似文献
4.
KB Cullberg T Christiansen SK Paulsen JM Bruun SB Pedersen B Richelsen 《Obesity (Silver Spring, Md.)》2013,21(3):454-460
Background:
Vascular growth is a prerequisite for adipose tissue (AT) development and expansion. Some AT cytokines and hormones have effects on vascular development, like vascular endothelial growth factor (VEGF‐A), angiopoietin (ANG‐1), ANG‐2 and angiopoietin‐like protein‐4 (ANGPTL‐4).Methods:
In this study, the independent and combined effects of diet‐induced weight loss and exercise on AT gene expression and proteins levels of those angiogenic factors were investigated. Seventy‐nine obese males and females were randomized to: 1. Exercise‐only (EXO; 12‐weeks exercise without diet‐restriction), 2. Hypocaloric diet (DIO; 8‐weeks very low energy diet (VLED) + 4‐weeks weight maintenance diet) and 3. Hypocaloric diet and exercise (DEX; 8‐weeks VLED + 4‐weeks weight maintenance diet combined with exercise throughout the 12 weeks). Blood samples and fat biopsies were taken before and after the intervention.Results:
Weight loss was 3.5 kg in the EXO group and 12.3 kg in the DIO and DEX groups. VEGF‐A protein was non‐significantly reduced in the weight loss groups. ANG‐1 protein levels were significantly reduced 22‐25% after all three interventions (P < 0.01). The ANG‐1/ANG‐2 ratio was also decreased in all three groups (P < 0.05) by 27‐38%. ANGPTL‐4 was increased in the EXO group (15%, P < 0.05) and 9% (P < 0.05) in the DIO group. VEGF‐A, ANG‐1, and ANGPTL‐4 were all expressed in human AT, but only ANGPTL‐4 was influenced by the interventions.Conclusions:
Our data show that serum VEGF‐A, ANG‐1, ANG‐2, and ANGPTL‐4 levels are influenced by weight changes, indicating the involvement of these factors in the obese state. Moreover, it was found that weight loss generally was associated with a reduced angiogenic activity in the circulation. 相似文献5.
An immuno-affinity method for the purification of mannose 6-phosphate receptor proteins 总被引:1,自引:0,他引:1
In a recent study, we have developed an ELISA method to quantify the mannose 6-phosphate receptor (MPR) proteins [J. Biochem. Biophys. Methods 52 (2002) 111]. In the present study, we have used the goat MPR 300 antibody and peptide specific antibodies to human MPR 46 to develop simple and efficient immuno-affinity matrices, which can be used to purify the MPR proteins from goat liver in a single step. The identity of the immuno-affinity purified receptors is confirmed by their molecular masses as well as by their immunoreactivity. 相似文献
6.
Janaína A Couto Karina LA Saraiva Cleiton D Barros Daniel P Udrisar Christina A Peixoto Juliany SB César Vieira Maria C Lima Suely L Galdino Ivan R Pitta Maria I Wanderley 《Reproductive biology and endocrinology : RB&E》2010,8(1):13
Background
The present study was designed to examine the effect of chronic treatment with rosiglitazone - thiazolidinedione used in the treatment of type 2 diabetes mellitus for its insulin sensitizing effects - on the Leydig cell steroidogenic capacity and expression of the steroidogenic acute regulatory protein (StAR) and cholesterol side-chain cleavage enzyme (P450scc) in normal adult rats. 相似文献7.
The Mannose 6-phosphate receptor (MPR’s) proteins are important for transporting lysosomal enzymes from trans-golgi to the pre-lysosomal compartment. These are conserved in the vertebrates from fish to mammals. We have cloned the full
length cDNA for the goat MPR 46 protein and compared its sequences to the other known vertebrate MPR 46 proteins. In the present
study the full-length cDNA for the goat MPR 46 protein was expressed in MPR deficient cells. The expressed protein was purified
on the multivalent phosphomannan gel in the presence of divalent metal ions. The apparent molecular mass of the expressed
protein was found to be ∼46 kDa and also exhibits oligomeric nature as observed in the other species, by using an MSC1 antibody
(that recognizes the MPR 46 from molluscs to mammals) as well as with a peptide specific antibody corresponding to amino acid
residues (218–237) of the cytoplasmic tail of human MPR 46 protein. Furthermore the distribution of the expressed protein
was visualized by immunofluorescence using MSC1 and LAMP1 antibody. Additionally in the goat MPR 46 expressing cells, the
sorting function of the expressed protein to sort cathepsin D to lysosomes was studied by confocal microscopy using cathepsin
D antiserum and LAMP1 antibody. The binding of goat MPR 46 to cathepsin D was shown in far Western blotting and the mannose
6-phosphate dependent binding was shown by co-immunoprecipitation. 相似文献
8.
9.
Koduru S Vegiraju SR Nadimpalli SK von Figura K Pohlmann R Dennes A 《Development genes and evolution》2006,216(3):133-143
Mannose-6-phosphate receptors (MPRs) have been identified in a wide range of species from humans to invertebrates such as
molluscs. A characteristic of all MPRs is their common property to recognize mannose-6-phosphate residues that are labelling
lysosomal enzymes and to mediate their targeting to lysosomes in mammalian cells by the corresponding receptor proteins. We
present here the analysis of full-length sequences for MPR 46 from zebrafish (Danio rerio) and its functional analysis. This is the first non-mammalian MPR 46 to be characterised. The amino acid sequences of the
zebrafish MPR 46 displays 70% similarity to the human MPR 46 protein. In particular, all essential cysteine residues, the
transmembrane domain as well as the cytoplasmic tail residues harbouring the signals for endocytosis and Golgi-localizing,
γ-ear-containing, ARF-binding protein (GGA)-mediated sorting at the trans-Golgi network, are highly conserved. The zebrafish
MPR 46 has the arginine residue known to be essential for mannose-6-phosphate binding and other additional characteristic
residues of the mannose-6-phosphate ligand-binding pocket. Like the mammalian MPR 46, zebrafish MPR 46 binds to the multimeric
mannose-6-phosphate ligand phosphomannan and can rescue the missorting of lysosomal enzymes in mammalian MPR-deficient cells.
The conserved C-terminal acidic dileucine motif (DxxLL) in the cytoplasmic domain of zebrafish MPR 46 essential for the interaction
of the GGAs with the receptor domains interacts with the human GGA1-VHS domain. Interestingly, the serine residue suggested
to regulate the interaction between the tail and the GGAs in a phosphorylation-dependent manner is substituted by a proline
residue in fish.
Electronic Supplementary Material Supplementary material is available for this article at .
The zebrafish MPR 46 sequence data have been submitted to the GenBank database under accession no. DQ089037. 相似文献
10.
Marcos G Godoy Alejandra Aedo Molly JT Kibenge David B Groman Carmencita V Yason Horts Grothusen Angelica Lisperguer Marlene Calbucura Fernando Avendaño Marcelo Imilán Miguel Jarpa Frederick SB Kibenge 《BMC veterinary research》2008,4(1):1-13