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排序方式: 共有112条查询结果,搜索用时 15 毫秒
1.
I L Shchaveleva I P Smirnov S V Kochetkova T L Tsilevich A A Khorlin 《Bioorganicheskaia khimiia》1988,14(6):824-827
Acyclic analogues of nucleosides, viz.9- and 3-(1,6-dihydroxy-4-oxahex-3-yl)adenine, 9-(1,6-dihydroxy-4-oxahex-3-yl)guanine, 1-(1,6-dihydroxy-4-oxahex-3-yl)cytosine and thymine, with C3'-C4' bond of the furanose ring cleaved, have been prepared by condensation of trimethylsilyl derivatives of nucleic acid bases with 1,4,6-triacetoxy-3-oxahexane in the presence of Lewis acids followed by treatment with metanolic ammonia. 相似文献
2.
I P Smirnov S V Kochetkova I L Shchaveleva T L Tsilevich B P Gottikh 《Bioorganicheskaia khimiia》1988,14(7):921-925
A convenient method has been proposed for the synthesis of 1,2-dihydroxy-4-oxahex-3-yl and 1-hydroxy-4-oxahex-3-yl derivatives of guanine, adenine, thymine and cytosine, acyclic nucleoside analogues lacking the 3'--4' bond. 相似文献
3.
Aminonucleosides and their derivatives. IV. Synthesis of the 3'-amino-3'-deoxynucleoside 5'-phosphates. 总被引:3,自引:3,他引:0 下载免费PDF全文
A V Azhayev A M Ozols A S Bushnev N B Dyatkina S V Kochetkova L S Victorova M K Kukhanova A A Krayevsky B P Gottikh 《Nucleic acids research》1979,6(2):625-643
A new procedure has been developed for the synthesis of 3'-amino-3'-deoxyribonucleosides of adenine, cytosine and uracil by condensing the trimethylsilylated bases with peracylated 3-azido-3-deoxyribose derivative. The azido group could subsequently be reduced to amino. The 5'-phosphates of these nucleosides have been prepared and the analogues have been tested for their ability to stimulate the ribosome-catalyzed reaction of 3'(2')-O-(N-formylmethionyl) adenosine 5'-phosphate with phenylalanyl-tRNA. 相似文献
4.
5.
Massimo Maddaloni Irina Kochetkova SangMu Jun Gayle Callis Theresa Thornburg David W. Pascual 《PloS one》2015,10(1)
Autoimmune diseases arise from the loss of tolerance to self, and because the etiologies of such diseases are largely unknown, symptomatic treatments rely on anti-inflammatory and analgesic agents. Tolerogenic treatments that can reverse disease are preferred, but again, often thwarted by not knowing the responsible auto-antigens (auto-Ags). Hence, a viable alternative to stimulating regulatory T cells (Tregs) is to induce bystander tolerance. Colonization factor antigen I (CFA/I) has been shown to evoke bystander immunity and to hasten Ag-specific Treg development independent of auto-Ag. To translate in treating human autoimmune diseases, the food-based Lactococcus was engineered to express CFA/I fimbriae, and Lactococcus-CFA/I fermented milk fed to arthritic mice proved highly efficacious. Protection occurred via CD39+ Tregs producing TGF-β and IL-10 to potently suppress TNF-α production and neutrophil influx into the joints. Thus, these data demonstrate the feasibility of oral nutraceuticals for treating arthritis, and potency of protection against arthritis was improved relative to that obtained with Salmonella-CFA/I. 相似文献
6.
7.
Bernal AJ Pan Q Pollack J Rose L Kozik A Willits N Luo Y Guittet M Kochetkova E Michelmore RW 《The Journal of biological chemistry》2005,280(24):23073-23083
Pto is a serine/threonine kinase that mediates resistance in tomato to strains of Pseudomonas syringae pv. tomato expressing the (a)virulence proteins AvrPto or AvrPtoB. DNA shuffling was used as a combinatorial in vitro genetic approach to dissect the functional regions of Pto. The Pto gene was shuffled with four of its paralogs from a resistant haplotype to create a library of recombinant products that was screened for interaction with AvrPto in yeast. All interacting clones and a representative sample of noninteracting clones were sequenced, and their ability to signal downstream was tested by the elicitation of a hypersensitive response in an AvrPto-dependent or -independent manner in planta. Eight candidate regions important for binding to AvrPto or for downstream signaling were identified by statistical correlations between individual amino acid positions and phenotype. A subset of the regions had previously been identified as important for recognition, confirming the validity of the shuffling approach. Three novel regions important for Pto function were validated by site-directed mutagenesis. Several chimeras and point mutants exhibited a differential interaction with (a)virulence proteins in the AvrPto and VirPphA family, demonstrating distinct binding requirements for different ligands. Additionally, the identification of chimeras that are both constitutively active as well as capable of binding AvrPto indicates that elicitation of downstream signaling does not involve a conformational change that precludes binding of AvrPto, as previously hypothesized. The correlations between phenotypes and variation generated by DNA shuffling paralleled natural variation observed between orthologs of Pto from Lycopersicon spp. 相似文献
8.
Binding of short fluorescently labeled AT-containing DNA duplexes with modified oligocytidylates is studied. The latter are modified to contain unnatural alpha-anomers along with natural beta-nucleotides; the nucleotide composition is selected according to putative pattern of unconventional triplex formation between duplex and oligomer bases. Nondenaturing gel electrophoresis is used to study complexation of fluorescent duplexes with cytidyl oligomers and oligocytidylate self-association at low temperatures. A DNA duplex of random AT composition is shown to bind with an excess of the corresponding oligocytidylate in 0.1 M Tris-HCl in the presence of Mg2+. Binding is observed at neutral pH values, while more basic pH (8.0) prevents complexation of the AT duplex and oligocytidylate. Contrary to oligonucleotides of irregular composition, a regular dA30:dT30 duplex does not bind with the dC strand. It is also shown that alternating self-complementary duplex d(AT)16 and oligocytidylate d(CbetaCalpha)15 do not form complexes, and poly-dC self-associates are formed instead. The effect of 2'-O-methylation of the third strand on complex formation and self-association is also analyzed. The results suggest that a modified oligocytidylate binds with a random-composition duplex, albeit with lower efficiency. 相似文献
9.
Koroleva NN Grishchuk IuV Kochetkova SV Prasolov VS Rubtsov PM Belzhelarskaia SN 《Molekuliarnaia biologiia》2006,40(6):1074-1080
The fragments of genomics DNA of the nuclear polyhedrosis virus (NPV) containing genes of late viral proteins p10, p35, p39, were cloned, the promoter regions of this genes were used to design baculovirus transfer vectors. A double-promoter and triple-promoter baculovirus transfer vectors were obtained. Recombinant baculovirus vectors containing mammalian expression cassette with cytomegalovirus (CMV) promoter, the gene for green or red fluorescent protein, SV40pA and polylinker MCS were constructed for the delivery of foreign genes into mammalian cells. 相似文献
10.
N. M. Mineeva A. S. Litvinov I. E. Stepanova M. Yu. Kochetkova 《Inland Water Biology》2008,1(1):64-72
On the basis of the data obtained during field observations in the summer low water period of 2001, the patterns of chlorophyll
distribution and its relation to hydrological and hydrochemical factors in two eutrophic reservoirs of the Middle Volga are
studied. The hydrological structure of the Gorky Reservoir, where the Volga water mass dominates, is homogeneous, while in
the Cheboksary Reservoir along with the eutrophic Volga waters, the mesotrophic Oka water masses can be distinguished keeping
their abiotic and biotic features over a long distance. Phytoplankton in the two contiguous reservoirs with different flow
regimens and anthropogenic loads responds differently to the external influence. An autotrophic community in the Gorky Reservoir
is more stable and depends little on abiotic factors which account for ∼63% of the explained chlorophyll variation. In the
Cheboksary Reservoir under maximal for the Volga cascade flow velocity and anthropogenic load, the development and distribution
of phytoplankton are almost completely (R
2 = 0.93) controlled by these factors. The trophic state of the reservoirs has not changed as compared to the beginning of
the 1990s. 相似文献