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排序方式: 共有93条查询结果,搜索用时 15 毫秒
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Cloning and characterization of a mouse cysteine proteinase 总被引:20,自引:0,他引:20
D A Portnoy A H Erickson J Kochan J V Ravetch J C Unkeless 《The Journal of biological chemistry》1986,261(31):14697-14703
cDNA clones encoding a mouse cysteine proteinase were isolated from a cDNA library constructed from mRNA derived from the macrophage-like cell line J774. The DNA sequence predicts a protein that is closely related to, but distinct from, the lysosomal enzyme cathepsin H. Alignment of the predicted amino acid sequence with the known protein sequences for seven other cysteine proteinases suggests that the cloned DNA encodes a 334-residue protein containing both a 17-amino acid pre-region and a 96-amino acid pro-region. Consistent with this prediction, antiserum raised to a recombinant fusion protein expressed in Escherichia coli immunoprecipitated multiple forms of the cysteine proteinase in mouse peritoneal macrophages and fibroblasts. In pulse-chase experiments, a 36-kDa precursor, presumedly the pro-form, was converted intracellularly into a 28-kDa protein and subsequently into a 21-kDa protein. Indirect immunofluorescence microscopy results suggested that the cysteine proteinase was localized to lysosomes. Western blot analysis detected significantly more of the proteinase in thioglycolate-elicited peritoneal macrophages than in resident peritoneal macrophages. Northern blot analysis revealed that several cell lines failed to express mouse cysteine proteinase mRNA. 相似文献
3.
E Vivier N Rochet J P Kochan D H Presky S F Schlossman P Anderson 《Journal of immunology (Baltimore, Md. : 1950)》1991,147(12):4263-4270
4.
Marta Dąbrowska Hanna Zielińska-Bliźniewska Paweł Kwiatkowski Łukasz Łopusiewicz Agata Pruss Mateusz Kostek Ewa Kochan Monika Sienkiewicz 《化学与生物多样性》2021,18(5):e2000843
One of the most common pathogens among yeasts is Candida albicans, which presents a serious health threat. The study aimed to check the antifungal properties of trans-anethole and eugenol with selected antifungal medicines (AMs) against C. albicans clinical isolates. The checkerboard method was used to tests of interactions between these compounds. Achieved results indicated that eugenol showed synergistic and additive activities with miconazole and econazole against investigated clinical isolates, respectively. Moreover, the combination – trans-anethole – miconazole also showed an additive effect against two clinical isolate. We tried to relate the results to changes in C. albicans cell sheaths under the influence of essential oils compounds (EOCs) performing the Fourier transform infrared spectra analysis to confirm the presence of particular chemical moieties in C. albicans cells. Nevertheless, no strong relationships was observed between synergistic and additive actions of used EOC-AMs combinations and chemical moieties in C. albicans cells. 相似文献
5.
Karolina Fryc Agnieszka Nowak Barbara Kij-Mitka Joanna Kochan Maciej Murawski Samantha Pena Pawel Mieczyslaw Bartlewski 《Animal Reproduction》2022,19(1)
The assessment of morphology and digital image opacity may provide valuable information on the present embryo quality. Time-lapse imaging has been employed in research to establish a means of monitoring the dynamic nature of preimplantation embryo development. The aim of present study was to use time-lapse imaging for assessing various prospective morphometric and phototextural markers of the developmental potential of in vitro-derived ovine embryos. Oocytes were obtained by scarification of ovaries from nine Polish Longwool ewes. After in vitro maturation (IVM) and fertilization (IVF) of oocytes with fresh ram semen, the development of embryos to the blastocyst stage was monitored and evaluated using Primo Vision time-lapse imaging technology. Commercially available Image-Pro® Plus software was used to measure zona pellucida thickness, embryo diameter, total area of the perivitelline space, cellular grey-scale pixel intensity and cellular pixel heterogeneity. Statistical assessment of all attributes was done at various time points during embryo development (i.e., presumptive zygote stage: t(0); first cleavage detected at t(2) or t(3); and second cleavage detected at t(4) or t(6)). Out of thirty-seven zygotes analyzed in this study, five did not divide, 26 arrested before and six developed to the blastocyst stage. Our present results indicate that most parameters analyzed did not differ among embryos varying in their developmental fate except for the perivitelline space area that was greater (P<0.05) for non-dividing zygotes than future blastocysts at the presumptive zygote stage (4040±1850 vs. 857±262 µm2, respectively; means±SEM). Consequently, the measurement of perivitelline space at t(0) can potentially be used to prognosticate developmental potential of in vitro-produced ovine embryos albeit further confirmational studies are needed. 相似文献
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A C Frantz A D McDevitt L C Pope J Kochan J Davison C F Clements M Elmeros G Molina-Vacas A Ruiz-Gonzalez A Balestrieri K Van Den Berge P Breyne E Do Linh San E O ?gren F Suchentrunk L Schley R Kowalczyk B I Kostka D ?irovi? N ?prem M Colyn M Ghirardi V Racheva C Braun R Oliveira J Lanszki A Stubbe M Stubbe N Stier T Burke 《Heredity》2014,113(5):443-453
Although the phylogeography of European mammals has been extensively investigated
since the 1990s, many studies were limited in terms of sampling distribution, the
number of molecular markers used and the analytical techniques employed, frequently
leading to incomplete postglacial recolonisation scenarios. The broad-scale genetic
structure of the European badger (Meles meles) is of interest as it may
result from historic restriction to glacial refugia and/or recent anthropogenic
impact. However, previous studies were based mostly on samples from western Europe,
making it difficult to draw robust conclusions about the location of refugia,
patterns of postglacial expansion and recent demography. In the present study,
continent-wide sampling and analyses with multiple markers provided evidence for two
glacial refugia (Iberia and southeast Europe) that contributed to the genetic
variation observed in badgers in Europe today. Approximate Bayesian computation
provided support for a colonisation of Scandinavia from both Iberian and southeastern
refugia. In the whole of Europe, we observed a decline in genetic diversity with
increasing latitude, suggesting that the reduced diversity in the peripheral
populations resulted from a postglacial expansion processes. Although MSVAR v.1.3
also provided evidence for recent genetic bottlenecks in some of these peripheral
populations, the simulations performed to estimate the method''s power to
correctly infer the past demography of our empirical populations suggested that the
timing and severity of bottlenecks could not be established with certainty. We urge
caution against trying to relate demographic declines inferred using MSVAR with
particular historic or climatological events. 相似文献
8.
Kochan TI 《Uspekhi fiziologicheskikh nauk》2007,38(1):55-65
The analysis of the results of our own researches and literature data allows us to single out two basic stages in the mechanism of human adaptation to the conditions of the North. They both are connected with circannual chronorhythm: 1) adaptation to decrease of daylight and ambient temperature: 2) adaptation to increase of insolation and to warming that gradually follows. Metabolic changes, characteristic for the first stage, lead to temporary hypoxia and hypoglycemia. These closely interconnected phenomena work, on the one hand, towards reduction of heat loss, and on the other--towards economical expenditure of energy of nutrients with the purpose of its reservation in the form of triglycerides. Lack of formation of heat in the respiratory chain as a result of partial inhibition of aerobic oxidation of substances is compensated by intensification of anaerobic glycolysis and glycogenolysis, which alongside with the increased utilization of glucose in lipogenesis leads to signs of hypoglycemia. The increase of insolation leads to activation of aerobic oxidation of reserve fats at synchronic intensification of gluconeogenesis. Adaptation changes of metabolism in the human organism during the year are studied in interrelation with functioning of cardiovascular system. 相似文献
9.
Soppa GK Smolenski RT Latif N Yuen AH Malik A Karbowska J Kochan Z Terracciano CM Yacoub MH 《American journal of physiology. Heart and circulatory physiology》2005,288(3):H1468-H1476
Clenbuterol (Clen), a beta(2)-agonist, is known to produce skeletal and myocardial hypertrophy. This compound has recently been used in combination with left ventricular assist devices for the treatment of end-stage heart failure to reverse or prevent the adverse effects of unloading-induced myocardial atrophy. However, the mechanisms of action of Clen on myocardial cells have not been fully elucidated. In an attempt to clarify this issue, we examined the effects of chronic administration of Clen on Ca(2+) handling and substrate preference in cardiac muscle. Rats were treated with either 2 mg x kg(-1) x day(-1) Clen or saline (Sal) for 4 wk with the use of osmotic minipumps. Ventricular myocytes were enzymatically dissociated. Cells were field stimulated at 0.5, 1, and 2 Hz, and cytoplasmic Ca(2+) transients were monitored with the use of the fluorescent indicator indo-1 acetoxymethyl ester. Two-dimensional surface area and action potentials in current clamp were also measured. We found that in the Clen group there was significant hypertrophy at the organ and cellular levels compared with Sal. In Clen myocytes, the amplitude of the indo-1 ratio transients was significantly increased. Sarcoplasmic reticulum Ca(2+) content, estimated by rapid application of 20 mM caffeine, was significantly increased in the Clen group. The action potential was prolonged in the Clen group compared with Sal. Carbohydrate contribution to the tricarboxylic cycle (Krebs cycle) flux was increased several times in the Clen group. This increase was associated with decreased expression of peroxisome proliferator-activated receptor-alpha. This study shows that chronic administration of Clen induces cellular hypertrophy and increases oxidative carbohydrate utilization together with an increase in sarcoplasmic reticulum Ca(2+) content, which results in increased amplitude of the Ca(2+) transients. These effects could be important when Clen is used in conjunction with left ventricular assist devices treatment. 相似文献
10.
Kołodziej Barbara Kochan Ewa Kazimierczak Jerzy Chmiel Aleksander 《Plant Growth Regulation》2006,48(1):13-19
American ginseng (Panax quinquefolium L.) is a perennial medicinal herb originally grown in Canada and USA, and recently also in China, Australia, Holland and
Poland. Several commercial preparations are produced from ginseng roots, that are known for their antifatigue, antitumor,
antistress and immune system stimulating functions. The medicinal properties are due mainly to the active components – ginsenosides.
In this work, the results of field cultivation experiments are presented that examine the effects of foliar application of
several growth regulators on quality parameters and ginsenoside content of P. quinuefolium roots. The growth regulators tested, i.e., kinetin, daminozide, mixture of gibberellic acid (GA3) with potassium salt of α-naphthalene acetic acid (kNAA) and new preparation – IPO-1 – benzimidazole derivative (obtained
from the Institute of Organic Industry in Warsaw – at present during the process of patent), were applied at a concentration
of 100 or 200 mg l−1 in the middle of June in the 2nd year of vegetation. After 4 years of cultivation, the roots were dug up and dried, and subsequently
the quantitative analysis of individual saponins (Rb1, Rb2, Rc, Rd, Re, Rg1) by HPLC was performed. Growth regulators significantly affected quality parameters, morphological features and accumulation
of individual and total ginsenosides in ginseng roots. Regardless of doses, the plant roots treated with growth regulators
had a higher content of total ginsenosides in comparison to the control. The growth regulators also affected individual ginsenosides
level and narrowed the ratio of Rb:Rg group. The application of kinetin, daminozide and benzimidazole derivative for foliar spray during 2nd year of American ginseng
vegetation caused a significant increase in air dry weight of roots and aboveground parts whereas the mixture of GA3 and kNAA showed a decreasing effect. An increase of roots size was observed using higher doses (200 mg l−1) of kinetin and daminozide while a decreasing tendency appeared with the application of the other preparations. 相似文献