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Konstantinova TV Diubankova NN Klykov VN Maslov MA Serebrennikova GA 《Bioorganicheskaia khimiia》2002,28(2):180-184
1,3-Dioxolane series cationic lipids containing residues of aliphatic or heterocyclic nitrogenous bases were synthesized. The bases were attached to the glycerol backbone either directly (piperazine) or via a spacer group through a thioether bond. 相似文献
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Z. U. Islam S. P. Klykov Z. Yu D. Chang E. B. Hassan H. Zhang 《Applied Biochemistry and Microbiology》2018,54(1):58-70
Pyrolysate obtained from the pyrolysis of waste cotton is a source of fermentable sugars that could be fermented into bioethanol fuel and other chemicals via microbial fermentation. However, pyrolysate is a complex mixture of fermentable and non-fermentable substrates causing inhibition of the microbial growth. The aim of this study was to detoxify the hydrolysate and then ferment it into bio-ethanol fuel in shake flasks and fermenter applying yeast strain Saccharomyces cerevisiae 2.399. Pyrolysate was hydrolyzed to glucose with 0.2 M sulfuric acid, neutralized with Ba(OH)2 followed by treatment with ethyl acetate and activated carbon to remove fermentation inhibitors. The effect of various fermentation parameters such as inoculum concentration, pH and hydrolysate glucose was evaluated in shake flasks for optimum ethanol fermentation. With respect to inoculum concentration, 20% v/v inoculum i.e. 8.0 × 108–1.2 × 109 cells/mL was the optimum level for producing 8.62 ± 0.33 g/L ethanol at 9 h of fermentation with a maximum yield of 0.46 g ethanol/g glucose. The optimum pH for hydrolysate glucose fermentation was found to be 6.0 that produced 8.57 ± 0.66 g/L ethanol. Maximum ethanol concentration, 14.78 g/L was obtained for 4% hydrolysate glucose concentration after 16 h of fermentation. Scale-up studies in stirred fermenter produced much higher productivity (1.32 g/L/h–1) compared to shake flask fermentation (0.92 g/L/h–1). The yield of ethanol reached a maximum of 91% and 89% of the theoretical yield of ethanol in shake flasks and fermenter, respectively. The complex of integrated models of development was applied, that has been successfully tested previously for the mathematical analysis of the fermentation processes. 相似文献
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It is shown that on the basis of the earlier revealed effect of generating the ion flow in the beam-plasma discharge from
the discharge axis, a plasma processing reactor can be created for low-energy etching of semiconductor structures. The possibility
of easily controlling the density and energy of ion flow by means of varying the potential of the discharge collector is demonstrated.
The charge compensation of the ion flow incident on the nonconducting surface is implemented using the modulation of the potential
of the substrate holder as well as the plasma-potential modulation. 相似文献
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Kazantseva V. V. Goncharuk E. A. Zaitsev G. P. Zagoskina N. V. Klykov A. G. 《Doklady. Biochemistry and biophysics》2022,502(1):10-14
Doklady Biochemistry and Biophysics - For the first time, the composition and the content of the main components of the phenolic complex of aboveground organs of buckwheat plants (Fagopyrum... 相似文献
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Efimov VA Klykov VN Chakhmakhcheva OG 《Nucleosides, nucleotides & nucleic acids》2007,26(10-12):1595-1599
A set of novel chiral pyrrolidine-based nucleotide mimics, in which nucleobase, hydroxyl group and phosphonic acid residue were attached to different carbon atoms of the pyrrolidine ring, was synthesized. These monomers were used for the synthesis of the corresponding oligomers, and their physico-chemical properties were evaluated. 相似文献
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Samgina TY Gorshkov VA Artemenko KA Vorontsov EA Klykov OV Ogourtsov SV Zubarev RA Lebedev AT 《Peptides》2012,34(2):296-302
Identification of species constituting Rana esculenta complex represents a certain problem as two parental species Rana ridibunda and Rana lessonae form their hybrid R. esculenta, while external signs and sizes of the members of this complex are intersected. However the composition of skin secretion consisting mainly of peptides is different for the species of the complex. LC-MS/MS is an ideal analytical tool for the quantitative and qualitative analysis of these peptides. The results covering elemental composition of these peptides, their levels in the secretion, as well as their belonging to a certain family of peptides may be visualized by means of 2D mass maps. The proposed approach proved itself to be a perspective tool for the reliable identification of all 3 species constituting R. esculenta complex. Easy distinguishing between the species may be achieved using 2D maps as fingerprints. Besides this approach may be used to study hybridogenesis and mechanisms of hemiclonal transfer of genetic information, when rapid and reliable identification of species involved in the process is required. 相似文献
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A number of cationic derivatives of cholesterol containing polar residues of N-methylimidazole, pyridine, N-methylmorpholine, and 4-N,N-dimethyaminopyridine were synthesized by the interaction of the corresponding heterocyclic bases with cholesterol 5-bromopentanoate followed by the treatment with methyl iodide in the case of tertiary amines. In addition, N-(4-cholesteryloxycarbonylbutyl)piperazine was obtained for the preparation of pH-sensitive liposomes. 相似文献
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V. A. Efimov A. V. Aralov V. N. Klykov O. G. Chakhmakhcheva 《Nucleosides, nucleotides & nucleic acids》2013,32(9):846-865
The azidomethyl and 2-(azidomethyl)benzoyl as 2′-OH protecting groups are reported for preparation of oligoribonucleotides by the phosphotriester solid-phase method using O-nucleophilic intramolecular catalysis. The procedures for the synthesis of the corresponding monomer synthons were developed and the usefulness of the application of 2′-O-azidomethyl and 2′-O-2-(azidomethyl)benzoyl groups was examined in the synthesis of different RNA fragments with a chain length of 15–22 nucleotides. The azidomethyl group was found to be more preferable for effective synthesis of oligoribonucleotides. Hybridization properties of RNAs toward their complementary oligonucleotides were examined before and after the removal of 2′-O-azidomethyl groups. 相似文献