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1.
D. Haferburg R. Hommel H.-P. Kleber S. Kluge G. Schuster H.-J. Zschiegner 《Engineering in Life Science》1987,7(4):353-356
A rhamnolipid released by Pseudomonas aeruginosa 196 Aa into the culture medium reduced the number of local lesions induced by tobacco mosaic virus on leaves of the hypersensitive host Nicotiana glutinosa L. by up to 90%. The content of potato virus X in the systemically infected host Nicotiana tabacum L. ‘Samsun’ is decreased in inoculated as well as in secondarily infected leaves by up to 50%. In a smaller degree red clover mottle virus is influenced in the systemic host Pisum sativumconvar.speciosum (Dierb.) Alef ‘Nadja’. 相似文献
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Rates of mutation to growth factor autonomy and tumorigenicity differ in hematopoietic stem and precursor cells expressing the multilineage colony-stimulating factor gene. 总被引:2,自引:0,他引:2 下载免费PDF全文
C Laker N Kluge C Stocking U Just M J Franz W Ostertag J F DeLamarter M Dexter E Spooncer 《Molecular and cellular biology》1989,9(12):5746-5749
At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic progenitor cells. The rate at which the second event occurs is more than 3 orders of magnitude higher in precursor cell lines (FDC-P1 or FDC-P2) than in stem cell lines (FDC-Pmix). Autonomous, but not density-dependent, growth is tightly coupled to tumorigenicity in precursor cells; however, neither growth-factor-independent nor autonomously growing stem cell lines are tumorigenic. 相似文献
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Manfred Kluge 《Planta》1967,73(1):50-61
Summary Sieve tube sap obtained from cucumber plants infected by Cucumis Virus 2 A induced the typical mosaic disease when it was inoculated into healthy plants of the same species. The infectious factor could not be removed by dialysis or by treatment with phosphodiesterase. Therefore it is improbable that the virus is transported in the sieve tubes as low molecular units or as an unprotected RNA. Rod shaped particles (345×23 m) were found in the infectious sieve tube sap when it was investigated by electron microscopy. The same particles could be found in the sap extracted from infected leaves, but never in sieve tube sap obtained from healthy plants. There is reason to suppose that the Cucumis Virus 2 A is transported in the sieve tubes as complete particles.
Einige Ergebnisse dieser Arbeit sind Teil einer Dissertation der Technischen Hochschule Darmstadt (D 17, 1964). 相似文献
Einige Ergebnisse dieser Arbeit sind Teil einer Dissertation der Technischen Hochschule Darmstadt (D 17, 1964). 相似文献
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S-Nitrosoglutathione in Rat Cerebellum: Identification and Quantification by Liquid Chromatography-Mass Spectrometry 总被引:1,自引:0,他引:1
Ina Kluge Ursula Gutteck-Amsler Markus Zollinger Kim Quang Do 《Journal of neurochemistry》1997,69(6):2599-2607
Abstract: Given the extreme lability and the facile inactivation of the messenger nitric oxide (NO) by many reactive biochemical species, it has been suggested that some intermediate compounds, for example, S -nitrosothiols, may act to stabilize NO and at the same time to preserve its biological activity. To test this hypothesis, we investigated if the S -nitrosothiol of glutathione, which is the predominant low molecular weight thiol in CNS, is present in the rat brain. The HPLC analysis of cerebellar extract from [35 S]cysteine-prelabeled slices suggested that S -nitrosoglutathione (GSNO) was indeed present in rat brain. To detect endogenous GSNO, a methodology based on liquid chromatography-mass spectrometry was developed. Besides an unequivocal identification of the endogenous GSNO, this method also permitted its precise quantification using 15 N-labeled GSNO ([15 N]-GSNO) as internal standard. GSNO level in adult cerebellum amounts to 15.4 ± 1.4 pmol/mg of protein. This is the first direct demonstration of the presence of endogenous GSNO in CNS. The packaging of NO in the form of GSNO might serve to facilitate its transport, prolong its life, and target its delivery to specific effectors. 相似文献
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C O-Yang D J Kertesz A F Kluge P Kuenzler T T Li M M Marx J J Bruno L F Chang 《Prostaglandins》1984,27(6):851-863
Opening of racemic epoxide (3) with (3S)- or (3R)-dimethyl-3-(dimethyl-t-butylsilyloxy)oct-1-ynyl aluminum gave two regioisomers, which were separated chromatographically. The separated regioisomers, themselves mixtures of chromatographically inseparable diastereoisomers, were converted into their dicobalthexacarbonyl complexes, which were easily resolved and isolated by chromatography. The individual diastereoisomers were deprotected to give bicyclo[3.2.0]heptan-3-ones, whose absolute stereochemistry was assigned using circular dichroism. One of these compounds, (1R,2R,3S,5R,3'S)-3-(3'-hydroxyoct-1'-ynyl)-bicyclo[3.2.0]++ +heptan-2-ol-6- oximinoacetic acid (11a) was 4.5 times more potent than PGE1 in inhibiting the ADP-induced aggregation of human platelets. The next most potent compound in this series was the "ent-15-epi" compound (11b), which was 0.034 times the potency of PGE1 in the platelet aggregation assay. 相似文献
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M. Kluge 《Oecologia》1977,29(1):77-83
Summary
Sedum acre L. collected from its natural stands south of Darmstadt (Germany) showed 13C values typical for C3 plants. This suggests that in situ at the natural stand CO2 was fixed mainly via the C3 mode of photosynthesis rather than via the CAM mode. However, experimental water stress shifts the CO2 exchange pattern from the C3 type to CAM type. Simultaneously, a diurnal rhythm of malic acid oscillation, typical for CAM, and increase of PEP-carboxylase and malic enzyme activities developed. Hence, Sedum acre is obviously to be classified as a facultative CAM plant. Because of the temperature characteristics of CO2 exchange in Sedum acre, in situ CO2 should be harvested from the atmosphere mainly during the seasons where water stress situations capable of inducing CAM are unlikely to occur. 相似文献
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M. Kluge D. Mollenhauer R. Mollenhauer R. Kape 《Plant biology (Stuttgart, Germany)》1992,105(5):343-344
The bladders of Geosiphon pyriforme, an endosymbiotic consortium of a fungus and the cyanobacterium Nostoc punctiforme, show nitrogenase activity. This suggests that the organism is capable of nitrogen fixation. 相似文献