Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients

Despite the growing understanding of the mechanisms of carcinogenesis, cancers of the central nervous system are usually associated with unfavorable prognosis. The use of an appropriate molecular marker may improve the treatment outcome by allowing early diagnosis and treatment susceptibility monitoring. Since methylation of tumor-derived DNA can be detected in the serum of cancer patients, this makes DNA methylation-based biomarkers one of the most promising diagnostic strategies. In this study, the methylation profiles of MGMT, RASSF1A, p15INK4B, and p14ARF genes were evaluated in serum free-circulating DNA and the corresponding tumor tissue in a group of 33 primary or metastatic central nervous system cancer patients. Gene promoter methylation was assessed using methylation-specific polymerase chain reaction (PCR). All the tested genes were found to be methylated to a different extent in both serum and tumor samples. In comparison to metastatic brain tumor patients, the patients with glial tumors were characterized by a higher frequency of gene hypermethylation. The hypermethylation of RASSF1A differentiated primary from metastatic brain cancers. Moreover, the gene methylation profiles observed in serum, in most cases, matched the methylation profiles detected in paired tumor samples.     

Restitution of vimba (Vimba vimba, Cyprinidae) in Poland: genetic variability of existing and restored populations

Until 1960s, vimba was found abundantly in Polish rivers and was an important species for the fishing industry. Overfishing, water pollution and dam constructions brought most of the vimba populations close to extinction. We analyzed the genetic variability of several remnant vimba populations in the Vistula and Oder river basins. The 305-bp fragment encompassing the 5′-end of the mtDNA control region was sequenced in a total of 202 wild specimens derived from six populations. Six different haplotypes were distinguished. The population of Barycz River was found to be the most polymorphic and was chosen as a source of breeding material for the vimba restitution program. In the years 2000–2008 fishes from this river were caught, spawned in captivity and released to the same river. The number of fishes returning to the Barycz River spawning grounds was monitored in the following years. In 2008, it was almost 70 times higher than the number of fishes recorded in 2003, when the lowest population size was observed. The haplotype and nucleotide diversities of the restored population were similar to those of the original population.     

Gonadal maturity in vimba (Vimba vimba L.) raised in carp ponds

The aim of the study was to determine gonadal maturity in male and female vimba after induced spawning in the years 2001 throughout 2004, based on spawners derived from an anadromous population obtained from the Barycz River. For these 4 years, fry derived from each successive spawning were raised in carp ponds at the Experimental Hatchery Centre of the Polish Angling Association in Szczodre, Poland. In February 2005, gonads were collected from males and females and their maturity determined after histological preparations. Additionally, in females the diameters of oocytes undergoing trophoplasmic growth were determined and compared. Histological analysis showed that all females had gonads at maturity stage IV, except the youngest fish, in which maturity stage I was determined. It was also shown that the size of oocytes undergoing trophoplasmic growth was related to age: the older the females, the greater the proportion of large‐diameter oocytes. In addition, some 2‐year‐old females had a considerable proportion of oocytes undergoing trophoplasmic growth despite being at stage IV of ovarian maturity. Among males, 2‐ to 4‐year‐olds had gonads at maturity stage III, in which the spermatogenesis process was active. One‐year‐old males had testes at maturity stage II, with visible spermatogonia entering meiotic divisions. When keeping vimba under pond conditions, only 2 years are needed to obtain a mature spawner from a spawning school. The older the school, the higher the probability that a greater number of spawners will enter spawning.