Plant Diversity in Hmong and Mien Homegardens in Northern Thailand

Plant Diversity in Hmong and Mien Homegardens in Northern Thailand. We surveyed 180 homegardens in three Hmong and three Mien villages in northern Thailand to study their floristic diversity and composition and to understand the impact of forced migration and ecological conditions for the development of the rich homegarden flora. We also looked at the role of culturally important species as carriers of cultural identity. We found 406 species: 341 (99 families) and 270 species (90 families) in Hmong and Mien homegardens, respectively. Five lowland villages had the richest homegarden flora, in part due to the presence of many widespread species. Twenty-two species were widespread in tropical countries and found in most Hmong and Mien homegardens, and 14 additional common species were shared between Hmong and Mien homegardens. Seventeen species were exclusive to Hmong homegardens and eight to Mien homegardens; these we have designated as culturally important species. The presence of culturally important species determines the homegardens’ characteristics and reflects the cultural identity related to plant uses of a group. Similarities in floristic composition of the two groups can be attributed to widespread and common species. Floristic variation and diversity in homegardens were strongly related to homegarden geographical location, personal preference, and cultural background of the owners.     

Utilization of Whole-Cell MALDI-TOF Mass Spectrometry to Differentiate Burkholderia pseudomallei Wild-Type and Constructed Mutants

Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (whole-cell MALDI-TOF MS) has been widely adopted as a useful technology in the identification and typing of microorganisms. This study employed the whole-cell MALDI-TOF MS to identify and differentiate wild-type and mutants containing constructed single gene mutations of Burkholderia pseudomallei, a pathogenic bacterium causing melioidosis disease in both humans and animals. Candidate biomarkers for the B. pseudomallei mutants, including rpoS, ppk, and bpsI isolates, were determined. Taxon-specific and clinical isolate-specific biomarkers of B. pseudomallei were consistently found and conserved across all average mass spectra. Cluster analysis of MALDI spectra of all isolates exhibited separate distribution. A total of twelve potential mass peaks discriminating between wild-type and mutant isolates were identified using ClinProTools analysis. Two peaks (m/z 2721 and 2748 Da) were specific for the rpoS isolate, three (m/z 3150, 3378, and 7994 Da) for ppk, and seven (m/z 3420, 3520, 3587, 3688, 4623, 4708, and 5450 Da) for bpsI. Our findings demonstrated that the rapid, accurate, and reproducible mass profiling technology could have new implications in laboratory-based rapid differentiation of extensive libraries of genetically altered bacteria.     

Viola umphangensis (Violaceae), a new species from Thailand

Viola umphangensis S. Nansai, Srisanga & Suwanph., a new species from Thailand, is described and illustrated. Morphological, anatomical and palynological characters of V. umphangensis are compared with a similar species, V. betonicifolia Sm. An amended key to the Viola species in the Flora of Thailand account is provided.