排序方式: 共有2条查询结果,搜索用时 0 毫秒
1
1.
Jo?o Vaz-Silva Rubens L. Tavares Márcia C. Ferreira Kinulpe Honorato-Sampaio Ines K. D. Cavallo Robson A. S. Santos Adelina M. dos Reis Fernando M. Reis 《Journal of molecular histology》2012,43(5):597-602
The vasoactive peptide angiotensin (Ang)-(1–7) has vasodilator, antifibrotic and antihypertrophic properties, but little is known about its regulation in the uterus. The aim of this study was to evaluate Ang-(1–7) and its receptor Mas expression throughout rat uterine tissues, in ovariectomized animals treated with estrogen alone or combined with progestin. Adult Wistar rats (n?=?19) were ovariectomized and randomly assigned into three different groups 1?week later. One group received a single dose of estradiol benzoate (1.5?mg/kg, i.m. injection, n?=?6). Another group received estradiol associated with depot medroxyprogesterone acetate (3?mg/kg, i.m. injection, n?=?6). Control group (n?=?7) received oil injection. One week later, the rats were euthanized and their uteri were fixed and stained by immunohistochemistry, using a polyclonal antibody specific to Ang-(1–7) and its receptor Mas. Ang-(1–7) was detected in all uterine tissues, but it was weak or absent in the circular myometrium of treated animals. The intensity of the immunostaining decreased in the glandular epithelium of hormonally treated animals when compared to controls. In estrogen treated rats, Ang-(1–7) labeling was scattered and sometimes included the nuclei of glandular cells. We also detected Ang-(1–7) expression in longitudinal myometrium and uterine serosa. Mas receptor was present in all tissues with similar intensity among the tissue types in the control and estrogen plus progestin groups. In the estrogen group, Mas staining was stronger in the luminal and glandular epithelium when compared with stroma or circular myometrium. In conclusion, ovarian steroids are not required to allow endometrial expression of Ang-(1–7) and its receptor Mas in rats, as it remains abundant in ovariectomized animals. However, estrogen and progestin may modulate the distribution pattern of this peptide in the endometrium, especially in the glandular compartment. 相似文献
2.
Comparative morphology of the oocyte surface and early development in four characiformes from the São Francisco River,Brazil
下载免费PDF全文
![点击此处可从《Journal of morphology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Kinulpe Honorato‐Sampaio Paula Suzanna Prado Yoshimi Sato Nilo Bazzoli Elizete Rizzo 《Journal of morphology》2015,276(10):1258-1272
Early development from the egg fertilization to complete resorption of the yolk‐sac is a critical period in the life cycle of teleost fish. Knowledge of this process provides essential parameters for aquaculture and identification of spawning sites in the wild. In the present study, a comparative morphological analysis of the oocyte surface as well as early development was performed in four commercially valuable species from the São Francisco River: Brycon orthotaenia, Leporinus obtusidens, Prochilodus argenteus, and Salminus franciscanus. Stripped oocytes, embryo, and yolk‐sac larvae were analyzed by scanning electron microscopy (SEM) and histology. A set of 10 lectins was used for investigation of lectin‐binding pattern in oocytes. In the four species, the outer layer of the zona radiata reacted to most lectins, indicating complex polysaccharides at the oocyte surface while no reactivity was detected in the inner zona radiata and yolk globules. Typical structural arrangements were recognized at the micropylar region by SEM. The four species showed nonadhesive eggs, short embryonic period (18–20 h at 24 ± 1°C), and poorly developed larvae at hatching. At 24 h posthatching (hph), larvae of the four species had neuromasts on the body surface. Rudimentary cement glands for larval attachment were identified on the cephalic region at 24 and 48 hph in B. orthotaenia and S. franciscanus, and following they were in regression. The time for whole yolk resorption varied among species from 48 to 120 hph, occurring earlier in S. franciscanus, followed by B. orthotaenia, P. argenteus, and L. obtusidens. The formation of the digestive tract and the mouth opening indicated initiation of exogenous feeding 24 h before complete resorption of the yolk. Together, our data indicate similarities in the early development among species that may be related to the life cycle strategies and phylogeny. J. Morphol. 276:1258–1272, 2015. © 2015 Wiley Periodicals, Inc. 相似文献
1