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1.
Zavadskaia ES Zakharova ES Kadulin SG Kibardin AV Kiselev SL Gnuchev NV 《Genetika》2001,37(9):1207-1212
Using the bovine alpha S1-casein gene, a genetic construct with an endostatin-coding fragment of the mouse collagen XVIII cDNA was designed to express endostatin in milk of transgenic animals. Several transgenic mice were obtained. The mice secreted endostatin in milk at 70-300 ng/microliter and transmitted this character to their progeny. 相似文献
2.
Various types of human blood cells were tested for expression of the Tag7/PGRP-SA and TagL/PGRP-L proteins, which belong to the family of proteins possessing the lysozyme-like peptidoglycan recognition protein (PGRP) domain. Expression regulation by several factors was demonstrated. 相似文献
3.
Zavadskaya E. S. Zacharova E. S. Kadulin S. G. Kibardin A. V. Kiselev S. L. Gnuchev N. V. 《Russian Journal of Genetics》2001,37(9):1009-1013
Using the bovine S1-casein gene, a genetic construct with an endostatin-coding fragment of the mouse collagen XVIII cDNA was designed to express endostatin in milk of transgenic animals. Several transgenic mice were obtained. The mice secreted endostatin in milk at 70–300 ng/l and transmitted this character to their progeny. 相似文献
4.
V M Kibardin L K Ebralidze A Iu Zvonarev 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1989,(1):61-63
The result obtained in the study of the possibility of using the method for the determination of the titer of antibodies to herpes simplex virus by EIA techniques in a single dilution of the serum under test are presented. This method is based on the determination of the optical density of the serum titer (rcut) in different groups of sera with the use of the assay system, permitting the evaluation of the positive results obtained in the determination of their final dilution. The results obtained with the use of this method showed that error was 50% for high-titer sera, 60% for medium-titer sera and 30% for low-titer sera. 相似文献
5.
Posviatenko AV Kulikova KV Gnuchev NV Georgiev GP Kibardin AV Larin SS 《Molekuliarnaia biologiia》2012,46(1):129-138
Colon carcinoma is a common type of neoplastic transformation. Mechanisms of its establishment and progression have been studying for several decades. Aberrant activation of the canonical Wnt signaling is frequently observed in colon carcinoma cells. Moreover, expression of the "noncanonical" Wnt ligands is also detected in this type of cancer. However, the implication of the noncanonical Wnt signaling in carcinogenesis and colorectal cancer (CRC) progression is still unclear. Here, to elucidate the characteristic features of the noncanonical Wnt signaling activation in CRC the expression of the "noncanonical" ligand hWnt11 has been studied. It was shown for the first time that expression of the hWnt11 in CRC is accompanied by the alternative splicing. The new hWnt11 isoform (hWnt11sp3) has been identified. Unlike to hWnt11, this isoform is not secreted and lacks the ability to inhibit the canonical Wnt signaling. Considering the canonical Wnt signaling inhibiting activity of hWnt11, different functional properties of the ligand and its isoform may reflect a special role of the alternative splicing in carcinogenesis and tumor progression. Thus, due to the difference in their functional properties an existence of several Wnt isoforms should be taken into account for the investigation of the role of Wnt ligands. 相似文献
6.
Targeted genetic modification of embryonic stem cells (ESC) was used to obtain nondifferentiated cell clones containing the foreign genetic material in the genome. It was demonstrated that transgenic animals may be obtained by ESC injection in preimplantation embryos and subsequent transplantation of the embryos into a recipient female. Using this method, we constructed chimeric animals with a modified genome. 相似文献
7.
Mirkina II Kibardin AV Korneeva EA Gnuchev NV Georgiev GP Kiselev SL 《Genetika》2000,36(11):1492-1500
The mouse tag7 gene was cloned and characterized in our previous works. This work is devoted to identifying and cloning its homologs. The human homolog of the tag7 gene was cloned. Data of analysis of the primary structure of the human tag7 and results from the study of the production of the corresponding protein in human organs and tissues indicate that this gene plays a major role in the mammalian immune system. The mouse and human tagL gene carrying an extended region of structural homology with the tag7 gene was detected by computer analysis and was subsequently cloned. Sequence analysis suggested the possible membrane localization of the gene, whereas the specific expression pointed to the role of this gene in the immune system. Both genes, tag7 and tagL, were localized in the human chromosome 19. 相似文献
8.
K. V. Kulikova A. V. Posvyatenko N. V. Gnuchev G. P. Georgiev A. V. Kibardin S. S. Larin 《Molecular Biology》2011,45(5):816-822
In most cases, advanced stages of melanoma are practically incurable due to high metastatic potential of tumor cells. Multiple
observations support the idea that aberrations in the Wnt signaling pathway play a significant role in melanoma development
and progression. Canonical Wnt signaling activation results in stabilization and accumulation of the major effector molecule
called & gb-catenin. Mutations promoting & gb-catenin stabilization and, thereby, activation of canonical Wnt signaling pathway
are frequently found in different cancers but rarely observed in melanomas. Nevertheless, & gb-catenin nuclear and cytoplasmic
accumulation is the feature of many human melanoma cell lines and original tumors. That is why the aim of the investigation
was to elucidate the relation between & gb-catenin intracellular localization and activity status of Wnt signaling pathway
in human melanoma cell lines. Ten human melanoma cell lines were characterized on the basis of the following parameters: canonical
Wnt ligand expression, intracellular & gb-catenin localization and activity status of canonical Wnt signaling pathway. Here,
it has been demonstrated that nuclear localization of & gb-catenin does not always correspond to active status of canonical
Wnt signaling pathway. Moreover, in the majority of cell lines with nuclear & gb-catenin, canonical Wnt signaling cannot be
activated by exogenous expression of an appropriate ligand. Human melanoma cell lines differ in activity of canonical Wnt
signaling pathway as well as in mechanisms of its regulation. Therefore, pathway-targeted potential antineoplastic therapy
requires the formation of a & ldmolecular pattern of cancer” for localization of the defect in Wnt signaling cascade in each
case. 相似文献
9.
A. V. Kibardin I. I. Mirkina I. R. Zakeyeva E. V. Baranova G. P. Georgiev S. L. Kiselev 《Russian Journal of Genetics》2003,39(2):185-189
Various types of human blood cells were tested for expression of the Tag7/PGRP-SA and TagL/PGRP-L proteins, which belong to the family of proteins possessing the lysozyme-like peptidoglycan recognition protein (PGRP) domain. Expression regulation by several factors was demonstrated. 相似文献
10.