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Lahiri V. L. Srivastava R. K. Hazra D. K. Gupta A. K. Painuly N. K. Sharma S. K. Khanna-Hazra P. Khanna P. Gupta R. K. Pathak Manish 《Cell biochemistry and biophysics》1994,24(1-3):9-14
Cell Biochemistry and Biophysics - Despite attempts to maintain asepsis, good manufacturing practices, and the use of terminal sterilization by millipore filtration, the nuclear practitioner is... 相似文献
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Hazra D. K. Lahiri V. L. Gupta A. K. Painuly N. K. Pathak Manish Khanna Pankaj Gupta R. K. Khanna-Hazra P. Saran Shabd 《Cell biochemistry and biophysics》1994,24(1-3):1-7
Radiosilver-111 and Radiogold-199 were proposed by us (1) as suitable isotopes for radioimmunotherapy in areas such as India by reason of their suitable half-lives and B-emissions
(Ag-111T
1/2=7.45 d and Au-199T
1/2=3.15 d). Since silver is monovalent, it is difficult to link to conventional bifunctional chelates. We therefore explored
the use of sulfur-based linkers (2). Encouraged by the Thakur and De Fulvio Technique (3) of linking technetium to disulfide
groups in antibodies reduced by ascorbic acid that is eminently biocompatible, we have explored the linkage of silver to immunoglobulin
reduced by ascorbic acid. The linkage of silver was assessed with stable Ag-108 using dialysis to quantify the free silver
after the reaction of silver and reduced immunoglobulins in various molar ratios (1∶1, 1∶2, 1∶5, 1∶10). The silver quantity
was estimated gravimetrically after precipitation as chloride. It was observed that using these molar ratios there was negligible
silver efflux into the dialysate, suggesting stable linkage. We also assessed the linkage using Ag-110M as radiotracer. The
comparative results with the two techniques are described. 相似文献
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