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1.
Kuniyoshi Masuda Tomio Kawata Kenji Takumi Takemi Kinouchi 《Microbiology and immunology》1980,24(6):507-513
The ultrastructure of a hexagonal array in the exosporium from spores of a highly sporogenic mutant of Clostridium botulinum type A strain 190L was studied by electron microscopy of negatively stained exosporium fragments using optical diffraction and filtration. The exosporium was composed of three or more lamellae showing an equilateral, hexagonal periodicity. Images of the single exosporium layer from which the noise had been filtered optically revealed that the hexagonally arranged, morphological unit of the exosporium was composed of three globular subunits about 2.1 nm in diameter which were arranged at the vertices of an equilateral triangle with sides of about 2.4 nm. The morphological units were arranged with a spacing of about 4.5 nm. The adjacent globular subunits appeared to be interconnected by delicate linkers. 相似文献
2.
Nontoxigenic variants were isolated from Clostridium botulinum type A strain 190L after treatment with detergents such as deoxycholate, sodium dodecyl sulfate, Tween 80 and Brij-58. Deoxycholate was most effective for obtaining the variants. The variants exhibited a markedly increased frequency of sporulation compared with the oligosporogenic parent strain. The cell wall of the parent strain was composed of an outer layer and an inner layer, whereas that of the variants lost the outer layer. After treatment with mitomycin C the parent strain was subjected to lysis and produced bacteriophages with a hexagonal head and a contractible tail, while the nontoxigenic variants did not yield bacteriophages or phage-like structures. There appears to be a close relationship among the toxigenic and sporogenic properties, formation of the outer cell wall layer and lysogeny. 相似文献
3.
Kenji Mori 《Bioscience, biotechnology, and biochemistry》2013,77(13):2563-2567
Methyl dl-12-homo-10-trans-juvenate (III) was synthesized from farnesol (IV) in eight steps involving stereoselective conversion of the trans-terminal methyl group to an ethyl group. The product (III) was less active than dl-C17-Cecropia juvenile hormone on both Tenebrio molitor and Galleria mellonella. 相似文献
4.
Tomonori Murakami Kenji Hiraoka Takeshi Mikami Tatsuji Matsumoto Susumu Katagiri Kunihiro Shinagawa Masuko Suzuki 《FEMS microbiology letters》1993,107(2-3):179-183
Abstract Flagellar antigen of Bacillus cereus H.1 was purified and tested for serodiagnostic antigen by ELISA. The antibody against the flagellar antigen of B. cereus H.1 reacted not only with the homologous specific antigen but also reacted with the flagellar antigens of 23 strains of B. cereus . This common flagellar antigen of B. cereus was found to be due to 61-kDa protein by SDS-PAGE and immunoblot assay. Monoclonal antibody H15A5 against common antigenic epitope of B. cereus also reacted with flagellar antigens of 21 strains of Bacillus thuringiensis by ELISA. This monoclonal antibody reacted with the 61-kDa protein of the flagella of B. cereus H.1 and H.2 and B. thuringiensis Kurstaki HD1, Alesti and Aizawai juroi by immunoblot analysis. These results indicated that the common antigenic epitope of the 61-kDa protein existed in the flagella both of B. cereus and B. thuringiensis . 相似文献
5.
Hidekatsu Yokoyama Shin-ichi Niwa Kenji Itoh Reiko Mazuka 《Biological cybernetics》1996,75(2):137-140
On the basis of a temporal model of animal behavior we conducted temporal analysis of eye movements in schizophrenic subjects
(n=10) and normal controls (n=10). We found a fractal property in schizophrenic subjects, the fixation time of eye movement during reading ambiguous and
difficult sentences showing a clear inverse power law distribution. An exponential distribution of a nonfractal nature was
found in normal controls.
Received: 21 July 1995/Accepted in revised form: 30 April 1996 相似文献
6.
Kitamura Akira; Matsui Kenji; Kajiwara Tadahiko; Hatanaka Akikazu 《Plant & cell physiology》1992,33(4):493-496
C6-Aldehydes emitted from intact tea leaves were analyzed quantitatively.Emission of the aldehydes increased temporarily in mid-May whenenzymatic activities involved in aldehyde formation from lipidsbegan to increase. Levels of C6-aldehydes in tea leaves alsoincreased temporarily. However, the accumulated C6-aldehydesdid not always correspond to emitted ones. (Received December 1, 1991; Accepted March 18, 1992) 相似文献
7.
8.
Complete nucleotide sequence of the mitochondrial DNA from a liverwort,Marchantia polymorpha 总被引:1,自引:1,他引:0
Kenji Oda Katsuyuki Yamato Eiji Ohta Yasukazu Nakamura Miho Takemura Naoko Nozato Kinya Akashi Takeshi Kanegae Yutaka Ogura Takayuki Kohchi Kanji Ohyama 《Plant Molecular Biology Reporter》1992,10(2):105-163
Libraries of cosmid and plasmid clones covering the entire region of mtDNA from the liverwortMarchantia polymorpha were constructed. These clones were used for the determination of the complete nucleotide sequence of the liverwort mtDNA
totally 186,608 bp (GenBank no. M68929) and including genes for 3 species of ribosomal RNAs, 29 genes for 27 species of transfer
RNAs, and 30 genes for functionally known proteins (16 ribosomal proteins, 3 subunits of cytochromec oxidase, apocytochromeb protein, 3 subunits of H+-ATPase, and 7 subunits of NADH ubiquinone oxidoreductase). The genome also contains 32 unidentified open reading frames.
Thus the complete nucleotide sequences from both chloroplast and mitochondrial genomes have been determined in the same organism.
Plasmid clones are available upon the request.
Gene names are represented according to Lonsdale and Leaver (1988) with modifications recommended by Lonsdale (personal communication). 相似文献
9.
Kenji Kato Su-wan Oh Hiroyuki Yamamoto Takayuki Hanazato Ikuko Yasuda Akira Otuki Masayuki Takahashi 《Ecological Research》1992,7(3):267-276
In order to understand the control mechanisms of a large, stable bacterial standing stock, enclosure experiments were conducted
in a eutrophic lake, where both bacterial productivity and grazing pressure were very high. Total bacterial number in the
different enclosures ranged from 1.2 to 2.7×107 cells mL−1 throughout the experiment. The average bacterial cell production rate estimated from a grazer eliminating experiment was
6.3×105 cells mL−1 h−1. Difference in the bacterial cell production rate between shaded and unshaded enclosures was not apparent. Bacteria showed
a reduction in standing stock of only about 25–30% even after the supply of light was cut to 1%. Bacteria in the shaded enclosures
then recovered their production rate in the first 12 days of perturbation. Grazing pressure in the shaded enclosures was not
less than that for the control. Thus, it was considered a control mechanism of bacterial stable standing stock that the bacteria
shifted their organic substrate from extracellular dissolved organic carbon freshly released from phytoplankton to that already
stocked in the water column, though it is not known whether the dominant bacteria were the same. 相似文献
10.
Summary We have isolated Saccharomyces cerevisiae mutants, smp, showing stable maintenance of plasmid pSRI, a Zygosaccharomyces rouxii plasmid. The smp mutants were recessive and were classified into at least three different complementation groups. The three mutants also showed increased stability of YRp plasmids and the mutations are additive for plasmid stability. One mutation, smp1, confers a respiration-deficient (rho
0) phenotype and several Rho– mutants independently isolated by ethidium bromide treatment of the same yeast strain also showed increased stabilities of pSR1 and YRp plasmids. The wild-type S. cerevisiae cells showed a strongly biased distribution of pSR1 molecules as well as YRp plasmids to the mother cells at mitosis, while the smpf mutant did not show this bias. Another mutation, smp3, at a locus linked to ade2 on chromosome XV, confers temperature-sensitive growth. The SMP3 gene encodes a 59.9 kDa hydrophobic protein and disruption of the gene is lethal. 相似文献