全文获取类型
收费全文 | 593篇 |
免费 | 23篇 |
专业分类
616篇 |
出版年
2021年 | 4篇 |
2018年 | 5篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2015年 | 13篇 |
2014年 | 16篇 |
2013年 | 19篇 |
2012年 | 36篇 |
2011年 | 40篇 |
2010年 | 18篇 |
2009年 | 20篇 |
2008年 | 25篇 |
2007年 | 33篇 |
2006年 | 30篇 |
2005年 | 31篇 |
2004年 | 25篇 |
2003年 | 30篇 |
2002年 | 24篇 |
2001年 | 11篇 |
2000年 | 16篇 |
1999年 | 14篇 |
1998年 | 10篇 |
1997年 | 10篇 |
1996年 | 11篇 |
1995年 | 8篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 11篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 8篇 |
1988年 | 13篇 |
1987年 | 6篇 |
1986年 | 6篇 |
1985年 | 4篇 |
1984年 | 9篇 |
1983年 | 6篇 |
1982年 | 8篇 |
1980年 | 7篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1975年 | 2篇 |
1974年 | 6篇 |
1972年 | 5篇 |
1971年 | 5篇 |
1970年 | 6篇 |
1968年 | 2篇 |
1966年 | 2篇 |
1965年 | 3篇 |
排序方式: 共有616条查询结果,搜索用时 0 毫秒
1.
K Takamiya 《Journal of biochemistry》1988,103(5):755-758
The presence of ubiquinol:cytochrome c2 oxidoreductase was shown in the membranes from a photosynthetic bacterium, Rhodopseudomonas palustris. Some properties of the enzyme in situ were investigated. The optimal pH of this enzyme activity was 7.0 in the intact membranes. The activity was inhibited by both antimycin and myxothiazol. Maximal activity (Vmax) was 3-4 mol cytochrome c (c2) reduced/mol cytochrome c1.s. Apparent activity of the enzyme with horse heart cytochrome c as the electron acceptor decreased as the concentration of salts in the reaction mixture increased, whereas when R. palustris cytochrome c2 was used as the electron acceptor, the activity increased as the concentration of salts increased. Moreover, the activity of the enzyme did not depend on the species or concentration of anions but on both the concentration and valency of the cations of the salts. These salt effects were thought to be due to the change of effective concentration of cytochrome molecules caused by cations near the membrane surface, which was net negatively charged. Apparent Km for ubiquinol-1 was about 80 microM irrespective of the species of cytochrome and the presence of salts. 相似文献
2.
Cytochrome b561 was removed from chromatophores of a photoanaerobicallygrown Rhodopseudomonas sphaeroides by deoxycholate-cholate andTriton X-100 treatments of the chromatophores. The cytochromewas purified by ammonium sulfate fractionation and gel filtration.Its molecular weight was 45,000 (45 kD) and it was composedof three subunits with molecular weights of 23 kD, 19 kD andless than 6 kD. The cytochrome preparation had absorption maximaat 414 nm in the oxidized form, and at 428, 530 and 561 nm inthe reduced form. Its pi was 4.8. The midpoint potential ofthis cytochrome was 153 mV at pH 7.0. The compound was autooxidizable,and it had cytochrome c oxidase activity. (Received May 16, 1983; Accepted September 8, 1983) 相似文献
3.
4.
5.
† Ken-ichiro Fukuchi †Tauni Ohman Nocthao Dang Anetta C. Smith ‡Clement E. Furlong George M. Martin 《Journal of neurochemistry》1996,66(5):2201-2204
Abstract: P19 is a C3H mouse-derived line of multipotent embryonic carcinoma cells that differentiate into neural cells. P19 cell clones overexpressing the three major forms of β-amyloid precursor protein from their cDNA constructs were established. Unlike a previous study in which P19-derived neurons had a limited α-secretase activity, all of these clones produced significant amounts of secreted β-amyloid precursor protein. When treated with retinoic acid, these transformed lines differentiated into neurons and survived better than did nontransformed parental P19 cells. Furthermore, P19-derived neurons survived better in medium conditioned by the transformed P19 line, and survival was reduced by immunoabsorption with an antibody to β-amyloid precursor protein. These results suggest neurotrophic effects of secreted β-amyloid precursor protein and contrast with a previous report in which overexpression of a full-length cDNA for β-amyloid precursor protein led to degeneration of P19-derived neurons. Western blot analysis suggested that this difference might result from different levels of expression of putative neurotoxic C-terminal fragments of β-amyloid precursor protein; moreover, P19-derived neurons differ from P19 stem cells in the processing of these C-terminal fragments. 相似文献
6.
Yoshihiko Sawa Ken-ichiro Shibata Mamoru Noda Tsuguo Watanabe 《Microbiology and immunology》1994,38(4):257-262
A 90 kDa protein of Mycoplasma salivarium was released from cell membranes of the organism with Triton X-100 and purified by ion-exchange chromatography and chromatofocusing. The protein was eluted at pH 5.5 by chromatofocusing. The protein was shown to react with the Fc fragments of IgG from human and nine different animal species and did not distinguish between IgG from different species, while protein A, tested for comparative purposes, displayed a strong specificity for human and swine IgG. Furthermore, the protein reacted with antigen specific goat IgG (specific for gamma chains of human IgG), sheep red blood cells (SRBC) sensitized with rabbit antiserum to SRBC, that is, the Fc part of rabbit IgG, and concanavalin A as well. These findings may suggest that the protein is a lectin which binds the carbohydrate moiety of the Fc part of IgG. 相似文献
7.
Ken-ichiro Takamiya Yuzo Shioi Masakazu Morita Hiroyuki Arata Minoru Shimizu Michio Doi 《Archives of microbiology》1993,159(1):51-56
Characteristics and occurrence of cytochrome c-552 from an aerobic photosynthetic bacterium, Roseobacter denitrificans, were described.Relative molecular mass of the cytrochrome was 13.5 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 15,000 by gel filtration. This cytochrome was a acidic protein having a pI of 5.6 and Em was +215 mV at pH 7.0. Absorption peaks were at 278, 408 and 524 nm in the oxidized form and 416, 523 and 552 nm in the reduced form.Amino acid composition and N-terminal amino acid sequence of cytochrome c-552 determined for 24 residues had low similarities to those of cytochrome c-551 of this bacterium, which is homologous to cytochrome c
2, although the physico-chemical properties of these two cytochromes were similar to each other.Cytochrome c-552 was maximally synthesized in the light under aerobic conditions but not in the dark. The synthesis also occurred in the presence of alternative acceptors such as trimethylamine N-oxide (TMAO) and nitrate under anaerobic conditions. Our results suggest that cytochrome c-552 is involved in TMAO respiration and denitrification in R. denitrificans, although the effect of light remains to be solved.Abbreviations Em
Midpoint redox potential
- PAGE
Polyacrylamide ge electrophoresis
- SDS-PAGE
Sodium dodecyl sulfate polyacrylamide gel electrophoresis
- TMAO
Trimethylamine N-oxide 相似文献
8.
Yoshihiro Izumi Kanji Ono Masayuki Takamiya Kiichi Fukui 《Journal of plant research》1993,106(4):319-325
Using cultured cells of the hornwortAnthoceros punctatus, the change in the relative chloroplast DNA content in each stage of chloroplast division was investigated to clarify the
relationship between the division cycle of a chloroplast and a cell nucleus. Samples of cultured cells were stained with 4′,6-diamidino-2-phenylindole
(DAPI) and then observed with an epifluorescence microscope and a chromosome image analyzing system (CHIAS). A chloropiast
in cultured cells duplicated DNA with an increase in size. When a chloroplast began to divide, it was constricted in the middle,
taking a dumbbell shape, and then divided into two daughter chloroplasts. In cultured cells of this species, the pattern of
quantitative change of chloroplast DNA, that is, the DNA replication pattern of chloroplasts, corresponded to that of cell
nuclear DNA in mitosis. 相似文献
9.
10.
Ohta Hiroyuki; Shimojima Mie; Ookata Kayoko; Masuda Tatsuru; Shioi Yuzo; Takamiya Ken-ichiro 《Plant & cell physiology》1995,36(6):1115-1120
Changes in the activity of UDP-galactose:diacylglycerol galactosyltransferase(UDGT), a key enzyme in galactolipid biosynthesis, during germinationwere investigated in cucumber (Cucumis sativus L. cv. Aonagajibai)seedlings. After germination, UDGT activity increased duringgrowth in darkness for 4 days, reaching 10 times the activityin ungerminated seeds. Illumination of 4-day-old dark-grownseedlings strongly stimulated the activity. By contrast, inseedlings grown continuously in darkness, the increase in UDGTactivity ceased after 4 days and the activity remained constantthereafter. A similar increase in the specific activity of UDGTwas observed i n the envelope fraction from seedlings, indicatingthat the increase in the enzymatic activity preceded synthesisof other proteins in the envelope membrane. Coincident withthe change in the enzymatic activity, here was an increase inlevels of monogalactosyl diacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), two major constituents of chloroplastmembrane lipids, in the germinated seedlings. Cycloheximideinhibited the light-mediated increase in the enzymatic activityby illumination of 4-day-old dark-grown seedlings, and, as aconsequence, it inhibited the accumulation of MGDG and DGDG.It was clear, therefore, that protein synthesis was necessaryduring this activation. Addition of a cytokinin, benzyladenine(BA), stimulated the increase in the UDGT activity. The increasein the UDGT activity caused by BA was accompanied by the accumulationof galactolipids, as in the case of the activation by light.These results suggest that activation of the final reactionin the synthesis of MGDG, which is catalyzed by the galactosyl-transferase,contributes to the accumulation of galactolipids during thedevelopment of the chloroplast membrane. (Received December 3, 1994; Accepted July 3, 1995) 相似文献