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排序方式: 共有1871条查询结果,搜索用时 15 毫秒
1.
Keisuke Hanaki Tomonori Matsuo Michihiko Nagase 《Biotechnology and bioengineering》1981,23(7):1591-1610
The inhibitory effect of long-chain fatty acids on the anaerobic digestion process was examined in batch experiments using synthetic substrates. The addition of long-chain fatty acids caused the appearance of the appearance of the lag period in the methane production from acetate and in the degradation of both long-chain fatty acids and n-butyrate. Methane production from hydrogen proceeded without lag period although its rate was lowered. Fermentation of glucose was not inhibited. Neutral fat in the whole milk was easily hydrolyzed to long-chain fatty acids, which brought about the inhibition. The addition of calcium chloride reduced the inhibitory effect of long-chain fatty but it did not do so after the culture had been exposed to long-chain fatty acids for more than several hours. The addition of calcium carbonate could not reduce the inhibition because of its insolubility. 相似文献
2.
Sleep and Biological Rhythms - 相似文献
3.
Hiroaki Nobuhara Keisuke Kuida Makoto Furutani Toshihiko Shiroishi Kazuo Moriwaki Yusuke Yanagi Tomio Tada 《Immunogenetics》1989,30(6):405-413
Southern blots of genomic DNA from 23 strains of laboratory mice and 19 individual wild mice were examined for restriction
fragment length polymorphisms in their loci encoding the T-cell receptors (Tcr): the constant regions of the α, β, and γ chains
(C
α,C
β, andC
γ) and a variable region family of the β chain (V
β8). Only a few polymorphisms were observed for each locus in the laboratory mice after using three restriction enzymes,Bam HI,Eco RI, andHind III. All the laboratory mice examined fall into one of two types for theC
α,C
β andV
β8 loci and one of three types for theC
γ. These types are found in some of the wild mice studied, indicating that they were already present in the founder mice of
laboratory mouse strains. In contrast, theTcr genes are highly polymorphic among wild mice. Analysis of the polymorphisms in these loci suggests that laboratory mice have
inherited their genes not only fromMus musculus domesticus, but also from other subspecies, and much more than previously believed from Asian subspecies. 相似文献
4.
Toshikuni Sasaoka Norio Kaneda Yoshikazu Kurosawa Keisuke Fujita Toshiharu Nagatsu 《Neurochemistry international》1989,15(4):555-565
The chromosomal gene for human phenylethanolamine N-methyltransferase (PNMT; EC 2.1.1.28) was isolated from a human genomic library using a cloned human PNMT cDNA as a probe, and the nucleotide sequence was determined. PNMT is encoded in a single gene which consists of three exons. We observed newly the presence of minor PNMT mRNA (type B) besides the major mRNA (type A) as reported previously (Kaneda et al., J. Biol. Chem. 263, 7672–7677, 1988) by Northern hybridization. Type B mRNA carries an approximately 700 nucleotide-long untranslated region in the 5′ terminus. This suggests that two types of mRNA are produced from a single gene through the use of two alternative promoters. A TATA-like sequence locates 30 base pair upstream from the cap site of type A mRNA. Upstream of the cap site, there are several sequences resembling Spl binding sites and glucocorticoid responsive elements, with the latter also found in the first intron. 相似文献
5.
Thiobarbituric acid-reactive substance formation of rat kidney brush border membrane vesicles induced by ferric nitrilotriacetate 总被引:4,自引:0,他引:4
S Hamazaki S Okada S Toyokuni O Midorikawa 《Archives of biochemistry and biophysics》1989,274(2):348-354
An iron chelate, ferric nitrilotriacetate (Fe3+-NTA), is nephrotoxic and also carcinogenic to the kidney in experimental animals. Iron-promoted lipid peroxidation in the proximal tubules is thought to be responsible for the pathologic process. In the present study, iron-promoted lipid peroxidation, with thiobarbituric acid (TBA) formation as an indication, in the tubular surface was simulated in vitro using rat kidney brush border membrane vesicles and the results were compared with those using linoleate micelles and rat liver microsomal lipid liposomes. Addition of ascorbate, cysteine, or dithiothreitol to the Fe3+-NTA solution resulted in consumption of dissolved oxygen and promoted the lipid peroxidation in the micelles and in the liposomes. In contrast, addition of glutathione to the Fe3+-NTA solution caused only sluggish oxygen consumption and far less peroxidation in these lipid systems. When the brush border membrane vesicles were used for the peroxidation substrate, Fe3+-NTA and glutathione could promote TBA formation at a rate comparable to that elicited by Fe3+-NTA with cysteine or dithiothreitol. Acivicin, a gamma-glutamyl transpeptidase inhibitor, suppressed the peroxidation of the brush border membrane vesicles promoted by Fe3+-NTA and glutathione. These results suggest the following mechanism of proximal tubular cell lipid peroxidation promoted by Fe-NTA: Fe3+-NTA filtered through glomeruli is rapidly reduced by cysteine and Fe2+-NTA starts lipid peroxidation at the site, leading to proximal tubular necrosis. Cysteine is amply supplied by the decomposition of glutathione within the lumen by the action of gamma-glutamyl transpeptidase and dipeptidase situated at the proximal tubular brush border membrane. 相似文献
6.
Calcium-dependent polymerization of human serum amyloid P component is inhibited by heparin and dextran sulfate 总被引:1,自引:0,他引:1
H Hamazaki 《Biochimica et biophysica acta》1989,998(3):231-235
The calcium-dependent polymerization of human serum amyloid P component (SAP) was spectrophotometrically monitored in 0.15 M NaCl at pH 7.5. The rate of the polymerization depended on the concentrations of SAP and Ca2+. It was shown for the first time that the calcium-dependent polymerization of SAP was inhibited by some sulfated polysaccharides. Most potent inhibitors were heparin and high molecular weight dextran sulfate of Mr 1.0.10(6). The inhibitory activity of glycosaminoglycans is accordant to their binding affinity for SAP, which was reported previously (Hamazaki, H. (1987) J. Biol. Chem. 262, 1456-1460). The polymerized SAP was reversibly dissociated by heparin and high molecular weight dextran sulfate. The results suggest that heparin and high molecular weight dextran sulfate may be a useful dissociating agent of polymerized SAP in amyloid deposits. 相似文献
7.
8.
Keisuke Yamano Masatomo Tagawa Evelyn Grace de Jesus Tetsuya Hirano Satoshi Miwa Yasuo Inui 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1991,161(4):371-375
Summary To clarify the hormonal regulation of metamorphosis of the conger eel (Conger myriaster), changes in whole body concentrations of thyroid hormones, thyroxine (T4) and triiodothyronine (T3), and cortisol during metamorphosis were examined, as well as the changes in the histological activity of the thyroid gland. In larvae before metamorphosis, T4 and T3 levels were less than 5 and 0.15 ng·g-1 respectively. Levels of T4 increased to about 30 ng·g-1 during early metamorphosis, and decreased subsequently. Levels of T3 increased gradually in early metamorphosis, and then increased abruptly to about 2.0 ng·g-1 in late metamorphosis. Before metamorphosis, cortisol levels of the leptocephali less than 11 cm in total length were greater than 200 ng·g-1. Cortisol levels decreased rapidly in larger premetamorphic leptocephali, and low levels were maintained throughout the metamorphic period. Histological observation revealed an activation of the thyroid gland in early metamorphosis; thyroid follicle epithelial cells became columnar and their nuclei larger. Active uptake of colloid by these cells and intensive vascularization of the gland were also observed. By the end of metamorphosis, follicle epithelial cells became squamous, indicating a low level of glandular activity. These results suggest that thyroid hormone plays an important role in regulation of conger eel metamorphosis.Abbreviations
AL
anal length
-
TL
total length
-
T
3
triiodothyronine
-
T
4
thyroxine 相似文献
9.
Kaoru Miyazaki Keisuke Mashima Nobuhiko Yamashita Jinpei Yamashita Takekazu Horio 《In vitro cellular & developmental biology. Plant》1985,21(1):62-66
Summary We have previously reported the transformation by Rous sarcoma virus of a cloned epithelial cell line (BRL) established from
Buffalo rat liver by H. Coon. The nontransformed (BRL) and transformed (RSV-BRL) cells grew at comparable rates in culture,
whereas only the transformed cells were tumorigenic in vivo. We report here on the existence in rat and mouse sera of a growth
inhibitor for the nontransformed BRL cells. The transformed BRL cells (RSV-BRL) were insensitive to this inhibitor. The inhibitory
activity was not prominent in sera from other species of animals tested except for rabbit; this serum inhibited the growth
of RSV-BRL cells more strongly than that of BRL cells. The growth inhibitor was partially purified from rat serum. It is a
protein free of lipid and has a molecular weight of about 220 000. The inhibitor could be separated into three components
of pI 4.6, 5.2 (major) and 5.6 by isoelectric electrophoresis.
EDITOR'S STATEMENT Although compelling theoretical arguments sometimes can be made for the likely existence of growth-inhibitory
substances of physical relevance in the control of cell proliferation, experiments aimed at identifying and studying such
factors often are difficult to design and interpret, and little strong data exists to suggest that growth-inhibitory substances
are important regulatorsin vivo. The information presented in this paper represents a start toward developing a useful system for studying growth-inhibitory
factor. David W. Barnes 相似文献
10.
Keisuke Kohmoto Kazuya Akimitsu Tetsuyuki Kohguchi Hiroshi Otani John M. Gardner 《Plant cell reports》1986,5(1):54-56
Amberlite XAD-7, a nonionic polyacrylate adsorbent, was found to be a very effective protectant for isolating mitochondria from tissues rich in oils and phenolics. Physiologically active, well-coupled mitochondria were successfully prepared from young green leaf tissues of citrus, apple, pear and tobacco.Abbreviations DNP
2,4-dinitrophenol
- CCCP
carbonyl cyanide m-chlorophenylhydrazone
- BSA
bovine serum albumin
- PVP
polyvinylpyrrolidone 相似文献