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Emanuela Viggiano Marcellino Monda Alessandro Viggiano Andrea Viggiano Caterina Aurilio Bruno De Luca 《Molecular and cellular biochemistry》2010,339(1-2):149-154
Previous studies have demonstrated that there is an increase in oxidative stress in the cerebral cortex of rats after repeated painful stimulation and that long-lasting pain increases the production of superoxide ion (O2 ?), nitric oxide and peroxynitrite due to the activation of AMPA and NMDA receptors. The purpose of the present study was to evaluate the possible role of O2 ? in the transmission of oro-facial pain. Formaldehyde 1% was injected subcutaneously into one vibrissal pad of adult male Sprague-Dawley rats as a model of persistent pain, then O2 ? production and superoxide dismutase (SOD) activity were evaluated in the left and right spinal trigeminal nuclei. O2 ? production was revealed using dihidroetidium (DHE) injected at 10 or 45 min after the formalin injection in conscious or anaesthetized rats. A histochemical assay for SOD was performed to evaluate the activity of SOD at 10 min after the formalin injection. The results showed a significant increase in O2 ? production in the homolateral nucleus at 45 min. However, there was no significant difference between the two sides at 10 min after the formalin injection. No significant difference was observed in SOD activity between the two sides of the spinal trigeminal nucleus. This study demonstrated that there is an increased production of O2 ? in the second phase but not in the first phase of the formalin test; thus O2 ? is involved in pain induced by inflammation, but not in acute pain. 相似文献
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Cornelis MC Monda KL Yu K Paynter N Azzato EM Bennett SN Berndt SI Boerwinkle E Chanock S Chatterjee N Couper D Curhan G Heiss G Hu FB Hunter DJ Jacobs K Jensen MK Kraft P Landi MT Nettleton JA Purdue MP Rajaraman P Rimm EB Rose LM Rothman N Silverman D Stolzenberg-Solomon R Subar A Yeager M Chasman DI van Dam RM Caporaso NE 《PLoS genetics》2011,7(4):e1002033
We report the first genome-wide association study of habitual caffeine intake. We included 47,341 individuals of European descent based on five population-based studies within the United States. In a meta-analysis adjusted for age, sex, smoking, and eigenvectors of population variation, two loci achieved genome-wide significance: 7p21 (P = 2.4 × 10(-19)), near AHR, and 15q24 (P = 5.2 × 10(-14)), between CYP1A1 and CYP1A2. Both the AHR and CYP1A2 genes are biologically plausible candidates as CYP1A2 metabolizes caffeine and AHR regulates CYP1A2. 相似文献
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Viggiano E Passavanti MB Pace MC Sansone P Spaziano G Viggiano A Aurilio C Monda M Viggiano A Pota V De Luca B De Luca E 《Journal of cellular physiology》2012,227(5):1988-1991
Glutamine (gln) is the most abundant free amino acid in the blood. It is involved in important metabolic and biochemical processes, like cell proliferation and oxidative stress. Previous studies have demonstrated that gln concentration in human plasma decreases in several conditions such as sepsis, ischemia-reperfusion, trauma, major surgery and burn. The aim of the present work was to compare the acute effects of different types of surgical interventions and of anesthetization on blood gln concentration. Plasma samples from 88 subjects (30 males and 58 females) were collected before and after major or minor surgery and the gln concentration was analyzed with high-performance liquid chromatography. The results showed that plasma gln concentration after surgery was lower than pre-surgery values and that in major surgery the decrease of gln was higher than in minor surgery. No significant effect was shown for sex or type of anesthesia. These results demonstrate the importance of a gln supplementation before a surgical intervention and show that the amount of gln supplementation should also be adjusted based on the type of surgery. 相似文献
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Monda Hiarhi Cozzolino Vincenza Vinci Giovanni Drosos Marios Savy Davide Piccolo Alessandro 《Plant and Soil》2018,429(1-2):407-424
Plant and Soil - The use of composted agricultural wastes as source of biostimulant compounds provides an added value to the recycling of biomasses. This study aims to expand the knowledge on the... 相似文献
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Objective: To use cluster analysis to create patterns of overall activity and inactivity in a diverse sample of Chinese youth and to evaluate their use in predicting overweight status. Research Methods and Procedures: The study populations were drawn from the 1997 and 2000 years of the longitudinal China Health and Nutrition Survey, comprised of 2702 and 2641 schoolchildren in the 1997 and 2000 cross‐sectional samples, respectively, and 1175 children in the longitudinal cohort. Cluster analysis was used to group children into nonoverlapping activity/inactivity “clusters” that were subsequently used in models of prevalent and incident overweight. Results were compared with traditional models, with activity and inactivity coded separately, to assess whether further insight was gained with the cluster analysis methodology. Results: Moderately and highly active youth were shown to have significantly decreased odds of overweight in both cross‐sectional and longitudinal analyses using cluster analysis. In incident longitudinal models, youth in the high activity/high inactivity cluster had the lowest odds of overweight [odds ratio = 0.12 (0.03, 0.44)]; in contrast, results from traditional models failed to show any significant relationship between overweight and activity or inactivity. Discussion: Cluster analysis methods allow researchers to simultaneously capture activity and inactivity in new ways. In this comparative study, only with the clustering methodology did we find a significant effect of activity on incident overweight, furthering our ability to examine this complex relationship. Interestingly, no effect of increasing levels of inactivity was observed using either method, indicating that activity seems to be the more important determinant of overweight in this population. 相似文献
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Viggiano A Viggiano E Monda M Viggiano A Ascione S Amaro S De Luca B 《Physiological research / Academia Scientiarum Bohemoslovaca》2008,57(2):269-273
Production of superoxide anions in the incubation medium of hippocampal slices can induce long-term potentiation (LTP). Other reactive oxygen species (ROS) such as hydrogen peroxide are able to modulate LTP and are likely to be involved in aging mechanisms. The present study explored whether intracerebro-ventricular (ICV) injection of oxidant or antioxidant molecules could affect LTP in vivo. With this aim in mind, field excitatory post-synaptic potentials (fEPSPs) elicited by stimulation of the perforant pathway were recorded in the dentate gyrus of the hippocampal formation in urethane-anesthetized rats. N-acetyl-L-cysteine, hydrogen peroxide (H2O2) or hypoxanthine/xanthine-oxidase solution (a superoxide producing system) were administrated by ICV injection. The control was represented by a group injected with saline ICV. Ten minutes after the injection, LTP was induced in the granule cells of the dentate gyrus by high frequency stimulation of the perforant pathway. Neither the H(2)O(2) injection or the N-acetyl-L-cysteine injection caused any variation in the fEPSP at the 10-min post-injection time point, whereas the superoxide generating system caused a significant increase in the fEPSP. Moreover, at 60 min after tetanic stimulation, all treatments attenuated LTP compared with the control group. These results show that ICV administration of oxidant or antioxidant molecules can modulate LTP in vivo in the dentate gyrus. Particularly, a superoxide producing system can induce potentiation of the synaptic response. Interestingly, ICV injection of oxidants or antioxidants prevented a full expression of LTP compared to the saline injection. 相似文献
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Satria P. Sajuthi Neeraj K. Sharma Jeff W. Chou Nicholette D. Palmer David R. McWilliams John Beal Mary E. Comeau Lijun Ma Jorge Calles-Escandon Jamehl Demons Samantha Rogers Kristina Cherry Lata Menon Ethel Kouba Donna Davis Marcie Burris Sara J. Byerly Maggie C. Y. Ng Nisa M. Maruthur Sanjay R. Patel Lawrence F. Bielak Leslie A. Lange Xiuqing Guo Michèle M. Sale Kei Hang K. Chan Keri L. Monda Gary K. Chen Kira Taylor Cameron Palmer Todd L. Edwards Kari E. North Christopher A. Haiman Donald W. Bowden Barry I. Freedman Carl D. Langefeld Swapan K. Das 《Human genetics》2016,135(8):869-880