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1.
The assembly of a large physical map of genomes requires simultaneous analysis of many cosmid clones for overlapping regions. The search for overlapping regions may be achieved by various means. High-performance liquid chromatography (HPLC) provides an alternative to gel electrophoresis since microgram amounts of each DNA fragment may be collected into individual test tubes for further analysis. HPLC has been used to identify overlapping cosmid clones from a pool of cosmid DNA containing the terminal portion of the long arm of the human X chromosome (Xq24-qter). Among 400 cosmids analyzed, 3 were shown to overlap. 相似文献
2.
[3H]Dihydroergocryptine ([3H]DHE) was shown to bind to sites in membranes from neuroblastoma X glioma hybrid cells (NG 108-15) that had the characteristics expected of alpha-adrenergic receptors. The binding was saturable with 0.3 pmol [3H]DHE bound per mg of protein and of high affinity, with an apparent dissociation constant (KD) of 1.8 nM. The specificity of the binding site for various ligands was more similar to that of alpha 2 receptors than to that of alpha 1. No specific binding of [3H]WB-4101 was found in the membranes derived from NG 108 cells. This finding also indicated that the [3H]DHE binding site in the cell is the alpha 2 receptor. GTP lowered the affinity of agonists for the [3H]DHE binding site, although the nucleotide hardly affected the affinity of antagonists including [3H]DHE. 相似文献
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Reiko Yu-Ito Kazuko Ôba Ikuzo Uritani 《Bioscience, biotechnology, and biochemistry》2013,77(8):2087-2091
In addition to HMG-CoA reductase, another HMG-CoA utilizing enzyme is present in the Mt fraction of sweet potato root tissue and its activity interferes with the assay to HMG-CoA reductase activity. 相似文献
5.
Michelle de C. S. Azevedo Patricia S. Rosa Cleverson T. Soares Luciana R. V. Fachin Ida Maria F. D. Baptista William J. Woods Gustavo P. Garlet Ana Paula F. Trombone Andrea de F. F. Belone 《PloS one》2015,10(12)
Jorge Lobo’s disease (JLD) is a chronic infection that affects the skin and subcutaneous tissues. Its etiologic agent is the fungus Lacazia loboi. Lesions are classified as localized, multifocal, or disseminated, depending on their location. Early diagnosis and the surgical removal of lesions are the best therapeutic options currently available for JLD. The few studies that evaluate the immunological response of JLD patients show a predominance of Th2 response, as well as a high frequency of TGF-β and IL-10 positive cells in the lesions; however, the overall immunological status of the lesions in terms of their T cell phenotype has yet to be determined. Therefore, the objective of this study was to evaluate the pattern of Th1, Th2, Th17 and regulatory T cell (Treg) markers mRNA in JLD patients by means of real-time PCR. Biopsies of JLD lesions (N = 102) were classified according to their clinical and histopathological features and then analyzed using real-time PCR in order to determine the expression levels of TGF-β1, FoxP3, CTLA4, IKZF2, IL-10, T-bet, IFN-γ, GATA3, IL-4, IL-5, IL-13, IL-33, RORC, IL-17A, IL-17F, and IL-22 and to compare these levels to those of healthy control skin (N = 12). The results showed an increased expression of FoxP3, CTLA4, TGF-β1, IL-10, T-bet, IL-17F, and IL-17A in lesions, while GATA3 and IL-4 levels were found to be lower in diseased skin than in the control group. When the clinical forms were compared, TGF-β1 was found to be highly expressed in patients with a single localized lesion while IL-5 and IL-17A levels were higher in patients with multiple/disseminated lesions. These results demonstrate the occurrence of mixed T helper responses and suggest the dominance of regulatory T cell activity, which could inhibit Th-dependent protective responses to intracellular fungi such as L. loboi. Therefore, Tregs may play a key role in JLD pathogenesis. 相似文献
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The axial growth of de-coated cocklebur (Xanthium pennsylvanicumWallr.) seeds, whose axes were divided into 4 zones, was examinedin relation to the temperature-dependent shift of the effectof C2H4 on germination. At 23?C, where both C2H4 and CO2 stimulatedgermination, CO2 promoted the axial growth at the radicle tipzone, whereas C2H4 promoted growth in the proximal portion ofthe axis. At 33?C, C2H4 inhibited germination, and stronglysuppressed the growth at the radicle tip, whereas the effectof CO2 did not change. The inhibition of growth at the radicletip zone was alleviated by O2 enrichment, which also reversedthe inhibition of germination. It is thus apparent that thetemperature-dependent shift of the action of C2H4 is associatedwith a temperature-dependent responsiveness of the radicle tipzone to C2H4. Growth of the radicle tip zone was sensitive toNaN3, whereas the proximal portion was sensitive to benzohydroxamicacid, an inhibitor of alternative respiration, suggesting thatthere may be an increase in the operation of the alternativerespiration path along a gradient of axial tissue from the tiptowards the cotyledonary side. The effects of CO2 and C2H4 arediscussed in relation to the different respiratory activitiesin each axial zone of cocklebur seeds. (Received May 9, 1986; Accepted November 6, 1986) 相似文献
8.
Heike Schmidt Rüdiger Bode Ida A. Samsonova Dieter Birnbaum 《Applied microbiology and biotechnology》1989,31(5-6):463-466
Summary Mutants of Candida maltosa were isolated that lacked saccharopine reductase (lys9) and saccharopine dehydrogenase (lys1) and were able to accumulate -aminoadipate--semialdehyde (AASA) in the cell and excrete it into the culture medium. The effects of incubation time, lysine concentration, and carbon and nitrogen sources on AASA production were examined. In the presence of 15 g glucose/1, 1.25 g NH4H2PO4/l and 50 mg l-lysine/l in a minimal salt medium C. maltosa G285 (lys1) produced about 80–90 mg AASA/l during 48 h of growth. A simple and rapid procedure to isolate AASA from the medium using Dowex 50X4 is described. 相似文献
9.
Heritability, phenotypic and genetic correlations of egg characteristics of Japanese quail were investigated to obtain basal information on breeding, strain identification and genetic monitoring. For this study, 3230 eggs produced from 323 female were used. Measurement were taken on egg weight, yolk weight, albumen weight, shell weight, egg shape index, albumen height, specific gravity, shell thickness, shell strength and yolk color. Heritability estimates of egg characteristics were high and ranged from 0.62 to 0.84. Diverse phenotypic and genetic correlations were observed among egg characteristics. These results indicate that individual selection should be the most efficient method of improving egg characteristics and that consideration should be given to the interrelationship of characteristics to accomplish genetic improvement. The possibility exists these assays of egg characteristics can be used for strain identification and genetic monitoring without killing an individual of quail. 相似文献
10.
Neonatal polycythemia is a perinatal complication in infants of diabetic mothers. The cord CBC (complete blood counts), serum iron, transferrin and ferritin concentrations were studied in newborn infants of 9 GDM (gestational diabetes), 21 NIDDM (noninsulin-dependent diabetes mellitus), and 8 IDDM (insulin-dependent diabetes mellitus) mothers. The RBC (red blood cell) count, Hb (hemoglobin) and Hct (hematocrit) of these infants were higher than control infants. There was no difference between the serum iron concentration of the infants of each group diabetic mothers and the infants in the control group, but the transferrin concentration was significantly higher and the ferritin was significantly lower in the infants of diabetic mothers than in those of control mothers. There was a significant negative correlation between transferrin and ferritin (r = -0.491 p less than 0.001). Erythropoiesis is considered to be enhanced in the fetuses of diabetic mothers, and the iron needed for erythropoiesis is reportedly transported from the mother to the fetus according to the demands of the fetus, but the iron storage was shown to be reduced in the fetuses of diabetic mothers. 相似文献