全文获取类型
收费全文 | 6007篇 |
免费 | 768篇 |
国内免费 | 6篇 |
出版年
2021年 | 68篇 |
2019年 | 68篇 |
2018年 | 68篇 |
2017年 | 73篇 |
2016年 | 86篇 |
2015年 | 192篇 |
2014年 | 200篇 |
2013年 | 239篇 |
2012年 | 302篇 |
2011年 | 325篇 |
2010年 | 194篇 |
2009年 | 180篇 |
2008年 | 263篇 |
2007年 | 258篇 |
2006年 | 264篇 |
2005年 | 229篇 |
2004年 | 231篇 |
2003年 | 259篇 |
2002年 | 238篇 |
2001年 | 152篇 |
2000年 | 159篇 |
1999年 | 152篇 |
1998年 | 86篇 |
1997年 | 64篇 |
1996年 | 68篇 |
1995年 | 68篇 |
1994年 | 63篇 |
1993年 | 53篇 |
1992年 | 137篇 |
1991年 | 107篇 |
1990年 | 106篇 |
1989年 | 88篇 |
1988年 | 96篇 |
1987年 | 98篇 |
1986年 | 93篇 |
1985年 | 95篇 |
1984年 | 80篇 |
1983年 | 70篇 |
1982年 | 64篇 |
1981年 | 62篇 |
1980年 | 63篇 |
1979年 | 93篇 |
1978年 | 55篇 |
1977年 | 68篇 |
1976年 | 47篇 |
1975年 | 51篇 |
1974年 | 56篇 |
1973年 | 65篇 |
1971年 | 49篇 |
1967年 | 46篇 |
排序方式: 共有6781条查询结果,搜索用时 15 毫秒
1.
2.
3.
Background
DNA Microarrays have become the standard method for large scale analyses of gene expression and epigenomics. The increasing complexity and inherent noisiness of the generated data makes visual data exploration ever more important. Fast deployment of new methods as well as a combination of predefined, easy to apply methods with programmer's access to the data are important requirements for any analysis framework. Mayday is an open source platform with emphasis on visual data exploration and analysis. Many built-in methods for clustering, machine learning and classification are provided for dissecting complex datasets. Plugins can easily be written to extend Mayday's functionality in a large number of ways. As Java program, Mayday is platform-independent and can be used as Java WebStart application without any installation. Mayday can import data from several file formats, database connectivity is included for efficient data organization. Numerous interactive visualization tools, including box plots, profile plots, principal component plots and a heatmap are available, can be enhanced with metadata and exported as publication quality vector files. 相似文献4.
Kay Terpe 《Applied microbiology and biotechnology》2013,97(24):10243-10254
During the genomics era, the use of thermostable DNA polymerases increased greatly. Many were identified and described—mainly of the genera Thermus, Thermococcus and Pyrococcus. Each polymerase has different features, resulting from origin and genetic modification. However, the rational choice of the adequate polymerase depends on the application itself. This review gives an overview of the most commonly used DNA polymerases used for PCR application: KOD, Pab (Isis?), Pfu, Pst (Deep Vent?), Pwo, Taq, Tbr, Tca, Tfi, Tfl, Tfu, Tgo, Tli (Vent?), Tma (UITma?), Tne, Tth and others. 相似文献
5.
6.
F Stephen Perry 《BMJ (Clinical research ed.)》1998,317(7156):481
7.
8.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
- Download : Download high-res image (147KB)
- Download : Download full-size image
- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
9.
The preparation and kinetics of lactate dehydrogenase attached to water-insoluble particles and sheets 总被引:2,自引:2,他引:0
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
1. The preparation of lactate dehydrogenase covalently attached to anion-exchange cellulose particles and sheets by use of a dichloro-sym-triazinyl dyestuff, Procion brilliant orange MGS, is described. 2. The stability and kinetic properties of these preparations were investigated. 3. An equation is derived to describe the change in concentration of a substrate when passed through a uniform bed of a substrate-inhibited enzyme. A number of theoretical curves are shown to illustrate the system. 4. A titrimetric assay for lactate dehydrogenase is described, and shown to be stoicheiometric over the range pH5.0-9.2. 5. The results are discussed in relation to previous work, and the effects of charged groups on the support, and of the diffusion film surrounding any particle in suspension, are treated qualitatively. 相似文献
10.