排序方式: 共有1条查询结果,搜索用时 0 毫秒
1
1.
Yoann Huet Jean-Pierre Ele Ekouna Aurore Caron Katiba Mezreb Michèle Boitel-Conti François Guerineau 《Biotechnology letters》2014,36(1):181-190
A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were constructed. One cassette was used to express the green fluorescent protein (GFP)-encoding gene in hairy roots grown in flasks. A stable and fast-growing hairy root line secreted GFP at >120 mg/l culture medium. GFP represented 60 % of the total soluble proteins in the culture medium. Turnip hairy roots retained sustainable growth and stable GFP production over 3 years. These results were superior to those obtained using tobacco hairy roots. 相似文献
1