全文获取类型
收费全文 | 3871篇 |
免费 | 358篇 |
国内免费 | 2篇 |
出版年
2023年 | 25篇 |
2022年 | 29篇 |
2021年 | 93篇 |
2020年 | 68篇 |
2019年 | 68篇 |
2018年 | 110篇 |
2017年 | 81篇 |
2016年 | 164篇 |
2015年 | 197篇 |
2014年 | 201篇 |
2013年 | 270篇 |
2012年 | 321篇 |
2011年 | 285篇 |
2010年 | 172篇 |
2009年 | 142篇 |
2008年 | 220篇 |
2007年 | 218篇 |
2006年 | 174篇 |
2005年 | 184篇 |
2004年 | 146篇 |
2003年 | 125篇 |
2002年 | 104篇 |
2001年 | 43篇 |
2000年 | 46篇 |
1999年 | 56篇 |
1998年 | 32篇 |
1997年 | 26篇 |
1996年 | 27篇 |
1995年 | 25篇 |
1994年 | 13篇 |
1993年 | 31篇 |
1992年 | 30篇 |
1991年 | 21篇 |
1990年 | 22篇 |
1989年 | 25篇 |
1988年 | 21篇 |
1987年 | 29篇 |
1986年 | 26篇 |
1985年 | 24篇 |
1984年 | 30篇 |
1983年 | 18篇 |
1982年 | 14篇 |
1981年 | 9篇 |
1980年 | 10篇 |
1979年 | 18篇 |
1978年 | 12篇 |
1975年 | 10篇 |
1974年 | 17篇 |
1973年 | 10篇 |
1970年 | 10篇 |
排序方式: 共有4231条查询结果,搜索用时 15 毫秒
1.
Background
Recent studies point to a great diversity of non-ribosomal peptide synthesis systems with major roles in amino acid and co-factor biosynthesis, secondary metabolism, and post-translational modifications of proteins by peptide tags. The least studied of these systems are those utilizing tRNAs or aminoacyl-tRNA synthetases (AAtRS) in non-ribosomal peptide ligation. 相似文献2.
3.
Reconstitution of RecBC DNase activity from purified Escherichia coli RecB and RecC proteins 总被引:8,自引:0,他引:8
I D Hickson C N Robson K E Atkinson L Hutton P T Emmerson 《The Journal of biological chemistry》1985,260(2):1224-1229
The Escherichia coli RecB protein, normally synthesized in low amounts, has been amplified by linkage of the recB gene to the phage lambda leftward promoter in an expression plasmid. From strains harboring this plasmid, RecB protein has been purified to homogeneity by a simple procedure which includes affinity chromatography on a column of RecC protein bound to agarose. The purified RecB protein has DNA-dependent ATPase activity but no exonuclease activity. RecC protein alone has neither ATPase nor exonuclease activity. However, when combined together, the RecB and RecC proteins show the ATP-dependent double-stranded exonuclease properties characteristic of the RecBC DNase. 相似文献
4.
5.
Fusarium graminearum A 3/5 possesses a high affinity system (Km = 32 +/- 8 microM; mean +/- SE) for uptake of choline, which was shown to be energy-dependent and constitutive. The maximum rate of choline uptake by this system was repressed by ammonia and glucose, showing a three-fold increase in maximum activity after nitrogen (2 h) or carbon (4 h) starvation. The system was highly specific for choline with only dimethylethanolamine (Ki = 198 +/- 29 microM), betaine aldehyde (Ki = 95 +/- 14 microM) and chlorocholine (Ki = 352 +/- 40 microM) acting as competitive inhibitors. Hemicholinium-3 acted as a mixed (non-competitive) inhibitor (KIES = 1.9 +/- 0.6 microM; KIE = 3.6 +/- 1.9 microM). 相似文献
6.
First appearing in 2000, and with 20,000 copies sold as it entered its sixth printing in 2004, Richard Trudgen's Why Warriors Lie down and Die is a minor publishing phenomenon. Although scholarly forums have generally ignored the book, Christian media outlets, the popular press and neo‐conservative political activists have enthusiastically received and promoted it. An examination of the merits of Why Warriors Lie down and Die suggests that its popularity is only partly explained by original observations or insights on the part of the author. The most important explanation, and implication, of the book's remarkable take‐up lies in the opportune way in which it corresponds with now ascendant neo‐conservative political perspectives on indigenous policy. 相似文献
7.
8.
9.
Kate Jolly 《BMJ (Clinical research ed.)》1991,303(6804):716-717
10.
Controlled generation of reactive oxygen species orchestrates numerous physiological signaling events (Finkel, T. (2011) Signal transduction by reactive oxygen species. J. Cell Biol. 194, 7–15). A major cellular target of reactive oxygen species is the thiol side chain (RSH) of Cys, which may assume a wide range of oxidation states (i.e. −2 to +4). Within this context, Cys sulfenic (Cys-SOH) and sulfinic (Cys-SO2H) acids have emerged as important mechanisms for regulation of protein function. Although this area has been under investigation for over a decade, the scope and biological role of sulfenic/sulfinic acid modifications have been recently expanded with the introduction of new tools for monitoring cysteine oxidation in vitro and directly in cells. This minireview discusses selected recent examples of protein sulfenylation and sulfinylation from the literature, highlighting the role of these post-translational modifications in cell signaling. 相似文献