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Muthu Vengaian Karmegam Sekar Karuppannan Denzil Britto Christopher Leslee Singaravadivel Subramanian Sivaraman Gandhi 《Luminescence》2020,35(1):90-97
A phenothiazine–rhodamine (PTRH) fluorescent dyad was synthesized and its ability to selectively sense Zn2+ ions in solution and in in vitro cell lines was tested using various techniques. When compared with other competing metal ions, the PTRH probe showed the high selectivity for Zn2+ ions that was supported by electronic and emission spectral analyses. The emission band at 528 nm for the PTRH probe indicated the ring closed form of PTRH, as for Zn2+ ion binding to PTRH, the λem get shift to 608 nm was accompanied by a pale yellow to pink colour (under visible light) and green to pinkish red fluorescence emission (under UV light) due to ring opening of the spirolactam moiety in the PTRH ligand. Spectral overlap of the donor emission band and the absorption band of the ring opened form of the acceptor moiety contributed towards the fluorescence resonance energy transfer ON mechanism for Zn2+ ion detection. The PTRH sensor had the lowest detection limit for Zn2+, found to be 2.89 × 10?8 M. The sensor also demonstrated good sensing application with minimum toxicity for in vitro analyses using HeLa cells. 相似文献
3.
Nripendra Vikram Singh Shilpa Parashuram Jyotsana Sharma Roopa Sowjanya Potlannagari Dhinesh Babu Karuppannan Ram Krishna Pal Prakash Patil Dhananjay M. Mundewadikar Vipul R. Sangnure P.V. Parvati Sai Arun Naresh V.R. Mutha Bipin Kumar Abhishek Tripathi Sathish Kumar Peddamma Harish Kothandaraman Sailu Yellaboina Dushyant Singh Baghel Umesh K. Reddy 《Saudi Journal of Biological Sciences》2020,27(12):3514-3528
4.
Chokkalingam Uvarani Karuppannan Arumugasamy Kumarasamy Chandraprakash Mathan Sankaran Athar Ata Palathurai Subramaniam Mohan 《化学与生物多样性》2015,12(3):358-370
Phytochemical investigation of the CHCl3 fraction of Swertia corymbosa resulted in the isolation of a new 3‐allyl‐2,8‐dihydroxy‐1,6‐dimethoxy‐9H‐xanthen‐9‐one ( 1 ), along with four known xanthones, gentiacaulein ( 3 ), norswertianin ( 4 ), 1,3,6,8‐tetrahydroxyxanthone ( 5 ), and 1,3‐dihydroxyxanthone ( 6 ). Structure of compound 1 was elucidated with the aid of IR, UV, NMR, and MS data, and chemical transformation via new allyloxy xanthone derivative ( 2 ). Compounds 1 – 6 exhibited various levels of antioxidant and anti‐α‐glucosidase activities. Absorption and fluorescence spectroscopic studies on 1 – 6 indicated that these compounds could interact with calf thymus DNA (CT‐DNA) through intercalation and with bovine serum albumin (BSA) in a static quenching process. Compound 1 was found to be significantly cytotoxic against human cancer cell lines HeLa, HCT116, and AGS, and weakly active against normal NIH 3T3 cell line. 相似文献
5.
Chidambaram Parameswari Rajasekaran Sripriya Karuppannan Veluthambi 《In vitro cellular & developmental biology. Plant》2010,46(5):395-402
In an analysis of 339 independent T
0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T
1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T
0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T
1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino
plants in the T
1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait
from the transgenic trait in the lines O54 and O36 was confirmed in T
2 and T
3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy
transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene
loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T
1 generation itself. Thus, we demonstrate that when an albino trait appears in the T
1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous
transgenic lines free from albinos can be established. 相似文献
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Ramachandran E Kalaivani P Prabhakaran R Rath NP Brinda S Poornima P Padma VV Natarajan K 《Metallomics : integrated biometal science》2012,4(2):218-227
New complexes, [Ni(HL)(PPh(3))]Cl (1), [Pd(L)(PPh(3))](2), and [Pd(L)(AsPh(3))](3), were synthesized from the reactions of 4-chloro-5-methyl-salicylaldehyde thiosemicarbazone [H(2)L] with [NiCl(2)(PPh(3))(2)], [PdCl(2)(PPh(3))(2)] and [PdCl(2)(AsPh(3))(2)]. They were characterized by IR, electronic, (1)H-NMR spectral data. Further, the structures of the complexes have been determined by single crystal X-ray diffraction. While the thiosemicarbazone coordinated as binegative tridentate (ONS) in complexes 2 and 3, it is coordinated as mono negative tridentate (ONS) in 1. The interactions of the new complexes with calf thymus DNA was examined by absorption and emission spectra, and viscosity measurements. Moreover, the antioxidant properties of the new complexes have also been tested against DPPH radical in which complex 1 exhibited better activity than that of the other two complexes 2 and 3. The in vitro cytotoxicity of complexes 1-3 against A549 and HepG2 cell lines was assayed, and the new complexes exhibited higher cytotoxic activity with lower IC(50) values indicating their efficiency in killing the cancer cells even at very low concentrations. 相似文献
9.
Ganapathi Sridevi Chidambaram Parameswari Natarajan Sabapathi Vengoji Raghupathy Karuppannan Veluthambi 《Plant science》2008,175(3):283-290
Agrobacterium-mediated transformation of rice was done using the binary vector pNSP3, harbouring the rice chitinase (chi11) gene under maize ubiquitin promoter and the tobacco β-1,3-glucanase gene under CaMV 35S promoter in the same T-DNA. Four of the six T0 plants had single copies of complete T-DNAs, while the other two had complex integration patterns. Three of the four single-copy lines showed a 3:1 segregation ratio in the T1 generation. Northern and western blot analyses of T1 plants revealed constitutive expression of chitinase and β-1,3-glucanase genes. Homozygous T2 plants of the single-copy lines CG20, CG27 and CG53 showed 62-, 9.6- and 11-fold higher chitinase activity over the control plants. β-1,3-Glucanase activity was 1.1- to 2.5-fold higher in the transgenic plants. Bioassay of homozygous T2 plants of the three single-copy transgenic lines against Rhizoctonia solani revealed a 60% reduction in sheath blight Disease Index in the first week. The Disease Index increased from 61.8 in the first week to 90.6 in the third week in control plants, while it remained low (26.8–34.2) in the transgenic T3 plants in the corresponding period, reflecting the persistence of sheath blight resistance for a longer period. 相似文献
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