全文获取类型
收费全文 | 1733篇 |
免费 | 139篇 |
出版年
2023年 | 7篇 |
2022年 | 20篇 |
2021年 | 39篇 |
2020年 | 21篇 |
2019年 | 26篇 |
2018年 | 29篇 |
2017年 | 34篇 |
2016年 | 54篇 |
2015年 | 75篇 |
2014年 | 105篇 |
2013年 | 108篇 |
2012年 | 148篇 |
2011年 | 148篇 |
2010年 | 93篇 |
2009年 | 80篇 |
2008年 | 124篇 |
2007年 | 136篇 |
2006年 | 115篇 |
2005年 | 100篇 |
2004年 | 128篇 |
2003年 | 95篇 |
2002年 | 79篇 |
2001年 | 11篇 |
2000年 | 12篇 |
1999年 | 18篇 |
1998年 | 16篇 |
1997年 | 11篇 |
1996年 | 10篇 |
1995年 | 8篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1985年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有1872条查询结果,搜索用时 31 毫秒
1.
2.
3.
Keven D. Juaire Karine Lapouge Matthias M.M. Becker Irina Kotova Michelle Michelhans Raphael Carapito Klemens Wild Seiamak Bahram Irmgard Sinning 《Structure (London, England : 1993)》2021,29(1):15-28.e7
- Download : Download high-res image (235KB)
- Download : Download full-size image
4.
Philippe Lejeune Philippe Bertin Corinne Walon Karine Willemot Charles Colson Antoine Danchin 《Molecular & general genetics : MGG》1989,218(2):361-363
Summary Spontaneous mutants of Escherichia coli K12 displaying an increased level of the kanamycin resistance conferred by plasmid pGR71 were selected. Several mutants obtained in this way apparently carry large chromosomal deletions extending into galU and/or bglY (27 min). This positive selection of deletions allowed detection of a new locus located between galU and bglY. Deletions of this locus are responsible for increased resistance to kanamycin (Irk), decreased resistance to l-serine in minimal medium (Drs) and decreased resistance to chloramphenicol (Drc) when a cat gene is present in the bacteria. 相似文献
5.
6.
Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype 总被引:17,自引:0,他引:17
7.
8.
Karine Cahier Damien Piel Rubén Barcia-Cruz David Goudenège K. Mathias Wegner Marc Monot Jesús L. Romalde Frédérique Le Roux 《Environmental microbiology》2023,25(8):1424-1438
Phages depend on their bacterial hosts to replicate. The habitat, density and genetic diversity of host populations are therefore key factors in phage ecology, but our ability to explore their biology depends on the isolation of a diverse and representative collection of phages from different sources. Here, we compared two populations of marine bacterial hosts and their phages collected during a time series sampling program in an oyster farm. The population of Vibrio crassostreae, a species associated specifically to oysters, was genetically structured into clades of near clonal strains, leading to the isolation of closely related phages forming large modules in phage–bacterial infection networks. For Vibrio chagasii, which blooms in the water column, a lower number of closely related hosts and a higher diversity of isolated phages resulted in small modules in the phage–bacterial infection network. Over time, phage load was correlated with V. chagasii abundance, indicating a role of host blooms in driving phage abundance. Genetic experiments further demonstrated that these phage blooms can generate epigenetic and genetic variability that can counteract host defence systems. These results highlight the importance of considering both the environmental dynamics and the genetic structure of the host when interpreting phage–bacteria networks. 相似文献
9.
Escherichia coli Heat Shock Protein DnaK: Production and Consequences in Terms of Monitoring Cooking
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Karine Seyer Martin Lessard Gabriel Piette Monique Lacroix Linda Saucier 《Applied microbiology》2003,69(6):3231-3237
Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F7010 of 1, 3, and 5 min), the intracellular concentration of DnaK in E. coli varied with the heating temperature (50 or 55°C). In fact, the highest DnaK concentrations were found after treatments at the lower temperature (50°C) applied for a longer time. Residual DnaK after heating was found to be necessary for cell recovery, and additional DnaK was produced during the recovery process. Overall, higher intracellular concentrations of DnaK tended to enhance cell resistance to a subsequent lethal stress. Indeed, E. coli cells that had undergone a sublethal heat shock (105 min at 55°C, F7010 = 3 min) accompanied by a 12-h recovery (containing 76,786 ± 25,230 molecules/cell) resisted better than exponentially growing cells (38,500 ± 6,056 molecules/cell) when later heated to 60°C for 50 min (F7010 = 5 min). Results reported here suggest that using stress protein to determine cell adaptation and survival, rather than cell counts alone, may lead to more efficient heat treatment. 相似文献
10.
Estimating estuarine residence times in the Westerschelde (The Netherlands) using a box model with fixed dispersion coefficients 总被引:13,自引:6,他引:7
The residence time of the water masses in the Westerschelde estuary was determined using a simple compartment-model that simulates the advective-diffusive transport of a conservative dissolved substance (chlorinity). The residence time of a water parcel in the upstream part of the estuary (i.e. the time needed for this water parcel to leave the estuary) varied from about 50 days in winter to about 70 days in summer. The most seaward compartment had residence times of about 10-15 days.Dispersive coefficients that are fixed in time were able to reproduce the observed salinity distributions very well in the Westerschelde. They were obtained by calibration on observed chlorinities. It is argued that the apparent relationship of dispersive coefficients with freshwater flow, which is observed in certain studies, could (partly) reflect the deviation from steady state conditions which are required assumptions to calculate these dispersive coefficients directly from salinity profiles. 相似文献