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1.
Josephine Abi-Ghanem Janet Chusainow Madina Karimova Christopher Spiegel Helga Hofmann-Sieber Joachim Hauber Frank Buchholz M. Teresa Pisabarro 《Nucleic acids research》2013,41(4):2394-2403
Site-specific recombinases (SSRs) can perform DNA rearrangements, including deletions, inversions and translocations when their naive target sequences are placed strategically into the genome of an organism. Hence, in order to employ SSRs in heterologous hosts, their target sites have to be introduced into the genome of an organism before the enzyme can be practically employed. Engineered SSRs hold great promise for biotechnology and advanced biomedical applications, as they promise to extend the usefulness of SSRs to allow efficient and specific recombination of pre-existing, natural genomic sequences. However, the generation of enzymes with desired properties remains challenging. Here, we use substrate-linked directed evolution in combination with molecular modeling to rationally engineer an efficient and specific recombinase (sTre) that readily and specifically recombines a sequence present in the HIV-1 genome. We elucidate the role of key residues implicated in the molecular recognition mechanism and we present a rationale for sTre’s enhanced specificity. Combining evolutionary and rational approaches should help in accelerating the generation of enzymes with desired properties for use in biotechnology and biomedicine. 相似文献
2.
Biology Bulletin - Abstract—The results of the study of 23 natural cenopopulations of Cephalaria uralensis (Murr.) Schrad. ex Roem. et Schult., a rare subendemic species of the Eastern... 相似文献
3.
Makarova OV Viktorova TV Ianbaeva DG Korytina GF Iakupova EV Karimova LK 《Genetika》2003,39(9):1268-1274
Genetic polymorphism of xenobiotic metabolizing enzymes responsible for individual susceptibility to different environmental factors was examined in a cohort of petrochemical workers occupationally exposed to adverse action of chemical compounds. Molecular genetic analysis of the 1462V mutation in exon 17 of the CYP1A gene demonstrated close similarity between the genotype and allele frequency distribution patterns in the industrial and control groups. No association between the CYP1A polymorphic alleles and genotypes and the duration of service and concomitant diseases was observed. The odds ratio of the disease development in the workers carrying heterozygous CYP1A1 mutant allele was 2.2. Analysis of the STM1 gene polymorphism demonstrated a decrease in the frequency of the homozygous deletion carriers in the workers compared to the control group. There were no substantial differences between the industrial and control groups with respect to the frequencies of rapid and slow acetylator genotypes revealed at the analysis of the NAT2 gene polymorphism. However, considering the concomitant diseases, in the corresponding industrial subgroup a clear trend towards lower frequency of rapid acetylators was demonstrated. In addition, the odds ratio of the disease development for the workers with slow acetylator phenotype was 1.7. 相似文献
4.
Paradoxical rescue from ischemic lung injury by inhaled carbon monoxide driven by derepression of fibrinolysis 总被引:43,自引:0,他引:43
Carbon monoxide (CO) can arrest cellular respiration, but paradoxically, it is synthesized endogenously by heme oxygenase type 1 (Ho-1) in response to ischemic stress. Ho-1-deficient (Hmox1-/-) mice exhibited lethal ischemic lung injury, but were rescued from death by inhaled CO. CO drove ischemic protection by activating soluble guanylate cyclase and thereby suppressed hypoxic induction of the gene encoding plasminogen activator inhibitor-1 (PAI-1) in mononuclear phagocytes, which reduced accrual of microvascular fibrin. CO-mediated ischemic protection observed in wild-type mice was lost in mice null for the gene encoding PAI-1 (Serpine1). These data establish a fundamental link between CO and prevention of ischemic injury based on the ability of CO to derepress the fibrinolytic axis. These data also point to a potential therapeutic use for inhaled CO. 相似文献
5.
The theory of natural focality of human diseases, basically formulated by Academician E.N. Pavlovsky in 1939, invigorated
research on plague, the disease whose epidemics had taken millions of lives in the past. Numerous studies in Russia and abroad
have provided a great amount of data on the pathogen, its carriers, and vectors, specific features of infection spread and
dynamics of activity of natural plague foci. Over a long period, plague was considered to be an obligate transmissible zoonosis.
However, recent laboratory experiments and direct observations in natural foci of this infection indicate that plague is probably
a zoophilous sapronosis. 相似文献
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7.
Karimova TV Maliavina TA Iakunina OIu 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2006,(5):82-83
Results of inspection 451 persons in the age of from 19 till 58 years and 2 children in the age of till 2 years on presence of specific antibodies and RNA of the infuenza virus A (H5N1) are submitted. RNA of infuenza virus A (H5N1) in researched samples is not revealed. Specific antibodies to a infuenza virus A (H5N1) in a blood serum of 211 patients and donors it is not revealed. 相似文献
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Nurtaza Aidana Magzumova Gulmira Yessimseitova Assel Karimova Venera Shevtsov Alexandr Silayev Dmitriy Lutsay Viktoriya Ramankulov Yerlan Kakimzhanova Almagul 《In vitro cellular & developmental biology. Plant》2021,57(6):965-976
In Vitro Cellular & Developmental Biology - Plant - Biodiversity conservation requires advanced and effective ex situ plant propagation techniques. The present study was conducted to optimize... 相似文献
10.
In Escherichia coli, cell division is performed by a multimolecular machinery called the divisome, made of 10 essential proteins and more than 20 accessory proteins. Through a bacterial two-hybrid library screen, we identified the E. coli β-lactam resistance protein Blr, a short membrane polypeptide of 41 residues, as an interacting partner of the essential cell division protein FtsL. In addition to FtsL, Blr was found to associate with several other divisomal proteins, including FtsI, FtsK, FtsN, FtsQ, FtsW, and YmgF. Using fluorescently tagged Blr, we showed that this peptide localizes to the division septum and that its colocalization requires the presence of the late division protein FtsN. Although Blr is not essential, previous studies have shown that the inactivation of the blr gene increased the sensitivity of bacteria to β-lactam antibiotics or their resistance to cell envelope stress. Here, we found that Blr, when overproduced, restores the viability of E. coli ftsQ1(Ts) cells, carrying a thermosensitive allele of the ftsQ gene, during growth under low-osmotic-strength conditions (e.g., in synthetic media or in Luria-Bertani broth without NaCl). In contrast, the inactivation of blr increases the osmosensitivity of ftsQ1(Ts) cells, and blr ftsQ1 double mutants exhibit filamentous growth in LB broth even at a moderate salt concentration (0.5% NaCl) compared to parental ftsQ1(Ts) cells. Altogether, our results suggest that the small membrane polypeptide Blr is a novel component of the E. coli cell division apparatus involved in the stabilization of the divisome under certain stress conditions. 相似文献