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Gizer Merve Köse Sevil Karaosmanoglu Beren Taskiran Ekim Z. Berkkan Aysel Timuçin Muharrem Korkusuz Feza Korkusuz Petek 《Biological trace element research》2020,193(2):364-376
Biological Trace Element Research - Metabolic diseases or injuries damage bone structure and self-renewal capacity. Trace elements and hydroxyapatite crystals are important in the development of... 相似文献
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Gökçinar-Yagci Beyza Karaosmanoglu Beren Taskiran Ekim Zihni Çelebi-Saltik Betül 《Molecular biology reports》2020,47(5):3833-3856
Molecular Biology Reports - In this study we used two different techniques in order to isolate pericytes from the wall of human umbilical cord vein and get two different groups of cells were named... 相似文献
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Demir Y Demir N Yildirim S Nadaroglu H Karaosmanoglu M Bakan E 《Preparative biochemistry & biotechnology》2001,31(3):291-304
In the present study, bone carbonic anhydrase was isolated from ancient human bones and its characteristic features were determined. For this purpose, the skull bone of about 3000 years age was used. The purification was performed in four steps. Four different isoenzymes of CA, including outer peripheral, inner peripheral, integral, and cytosolic were purified and characterized. Affinity chromatography using Sepharose-4B-L-tyrosyn sulfanilamide as a support material was used in its purification. Two different methods were used for enzymatic activity determination: a) hydratase, and b) esterase methods. Bradford and Coomassie Brillant Blue methods were used for protein determination. Optimal pH, temperature, and molecular weight determinations were performed by conventional methods. The purification degree and the subunits, if present, were determined by SDS-PAGE. The effects of some chemicals on the enzyme were also investigated. The most cardinal finding was that the enzymatic activity has been found in antique human bone, showing some other enzymatic activity. That the alkaline phosphatase activity has been determined in the same sample supports the finding of carbonic anhydrase. 相似文献
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