排序方式: 共有28条查询结果,搜索用时 406 毫秒
1.
Phongphat Obounchoey Lueacha Tabtimmai Praphasri Suphakun Kannika Thongkhao Chatchakorn Eurtivong Matthew Paul Gleeson Kiattawee Choowongkomon 《Journal of cellular biochemistry》2019,120(3):3353-3361
The epidermal growth factor receptor (EGFR) was found to be overexpressed in several cancers, especially in lung cancers. Finding new effective drug against EGFR is the key to cancer treatment. In this study, the GOLD docking algorithm was used to virtually screen for novel human EGFR inhibitors from the NCI database. Thirty-four hit compounds were tested for EGFR-tyrosine kinase (TK) inhibition. Two potent compounds, 1-amino-4-(4-[4-amino-2-sulfophenyl]anilino)-9,10-dioxoanthracene-2-sulfonic acid (NSC125910), and nogalamycin N-oxide (NSC116555) were identified with IC50 values against EGFR-TK comparable to gefitinib; 16.14 and 37.71 nM, respectively. However, only NSC116555 demonstrated cytotoxic effects against non–small-cell lung cancer, A549, shown in the cell cytotoxicity assay with an IC50 of 0.19 + 0.01 µM, which was more potent than gefitinib. Furthermore, NSC116555 showed cytotoxicity against A549 via apoptosis in a dose-dependent manner. 相似文献
2.
Siripattarapravat K Pinmee B Chang EA Muñoz JD Kawakami K Cibelli JB 《The International journal of developmental biology》2010,54(11-12):1679-1683
The success of nuclear reprogramming following somatic cell nuclear transfer (SCNT) is thought to depend on factors present in the egg. Little is known about the role - if any - played by the somatic cell type on the outcome of the procedure. We tested whether cells of different lineages might have different capacities for reprogramming following SCNT, comparing cells isolated from five different tissues of transgenic zebrafish for their developmental potential when used as SCNT donor cells. We used transgenic zebrafish lines expressing green fluorescence protein under an endogenous tissue-specific promoter: HGn62A-skin, HGn28A-skin, HGn8E-heart, HG21C-fin and notochord and HGn30A-hatch gland. We analyzed the efficiency of cloning, as measured by reconstructed embryos that developed up to the hatched-fry stage. Specifically, donor cells of fin and notochord origin yielded the best rate of cloned fish production. All of the other cell types used were capable of producing cloned fish, albeit with significantly lower efficiency. These results indicate that the type of zebrafish cells used for SCNT can influence the outcome of the procedure. Future epigenetic analysis of these cells will help determine specific chromatin profiles in somatic cells that have an impact on nuclear reprogramming procedures. 相似文献
3.
4.
Molecular serotyping,virulence gene profiling and pathogenicity of Streptococcus agalactiae isolated from tilapia farms in Thailand by multiplex PCR 下载免费PDF全文
5.
Histomorphological studies of the testis and male genital ducts of Supachai's caecilian,Ichthyophis supachaii Taylor, 1960 (Amphibia: Gymnophiona) 下载免费PDF全文
Akkanee Pewhom Pramote Chumnanpuen Itsares Muikham Kannika Chatchavalvanich Nopparat Srakaew 《Acta zoologica》2016,97(1):76-89
We investigated the structure of the male reproductive system in Ichthyophis supachaii. The testis comprises a series of mulberry‐like lobes, each of which contains testis lobules occupied by germ cysts. A single cyst consists of synchronously developing germ cells. Six spermatogenic cell types, viz. primary spermatogonia, secondary spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa, have been identified and described. Notably, the testis of I. supachaii encompasses specific organization patterns of spermatids and spermatozoa during spermiogenesis. Spermiating cysts rupture and release spermatozoa to the collecting ducts, which are subsequently transported to the sperm duct, Wolffian duct and cloaca. We report for the first time ciliated cells in the epithelium of the caecilian Wolffian duct. The cloaca is divided into the urodeum and phallodeum. The urodeum has ciliated and glandular epithelia at its dorsolateral and ventral regions, respectively, as the lining of its internal surface. The muscular phallodeum is lined by ciliated epithelium. Paired Mullerian ducts lie parallel to the intestine and join the cloaca. The posterior portion of the duct is modified as the Mullerian gland. The most posterior region is non‐glandular and lined by ciliated epithelium. Our findings contribute further to information on the reproductive biology of caecilians in Thailand. 相似文献
6.
Sirilug Magerd Monsicha Somrit Parichat Prachaney Amara Thongpan Kannika Chatchavalvanich Prapee Sretarugsa Wattana Weerachatyanukul 《Cell and tissue research》2009,336(2):267-276
Our aim has been to determine whether carbohydrate distribution in the oviducts of progesterone-treated animals is comparable
with that of seasonal breeders in Rana tigrina. Like many other anurans, R. tigrina oviduct exhibits a short straight portion (pars recta, pr) at the beginning followed by a long, highly coiled portion (pars
convoluta, pc). Histologically, the oviduct of this species revealed some unique features, one of which was intense toluidine
blue staining, specifically in the upper mucosal glands of pc4. Based on lectin reactivities in the epithelial cells and mucosal
glands, patterns of lectin staining in the seasonal breeders were classified into seven types: R1-R3 (for pr) and C1-C4 (for
pc). Typically, some lectins reacted selectively either with ciliated cells (concanavalin A) or non-cialiated cells (Ricinus communis agglutinin I and wheatgerm agglutinin); however, Bandeiraea simplicifolia agglutinin I reacted with both cell types. These staining patterns were different in the progesterone-treated animals. Differences
in glycan distribution in the oviductal secretions were revealed by lectin blotting. Compared with the seasonal breeders,
an enhanced staining of some lectins was noted in the hormone-treated animals: either an increased staining intensity of existing
protein bands or additional staining of new protein bands. Inversely, the staining of wheatgerm agglutinin was markedly diminished
in the hormone-treated animals, suggesting the inhibitory effect of progesterone on oviductal glycan distribution. Whether
alteration in glycan distribution upon progesterone treatment affects the physiological properties of the released jelly substances
remains to be addressed.
This research was supported by Thailand Research Funds (to W.W.), a Research Initiate Grant from Kasetsart University (to
A.T.), and Mahidol University. 相似文献
7.
Uraiwan Panich Kamolratana Kongtaphan Tassanee Onkoksoong Kannika Jaemsak Rattana Phadungrakwittaya Athiwat Thaworn Pravit Akarasereenont Adisak Wongkajornsilp 《Cell biology and toxicology》2010,26(2):103-116
Ultraviolet A (UVA) irradiation is suggested to contribute to melanogenesis through promoting cellular oxidative stress and
impairing antioxidant defenses. An overproduction of melanin can be associated with melanoma skin cancer and hyperpigmentation.
Therefore, developing effective antimelanogenic agents is of importance. Alpinia galanga (AG) and Curcuma aromatica (CA) are traditional medicinal plants widely used for skin problems. Hence, this study investigated the antimelanogenic effects
of AG and CA extracts (3.8–30 μg/ml) by assessing tyrosinase activity, tyrosinase mRNA levels, and melanin content in human
melanoma cells (G361) exposed to UVA. The roles in protecting against melanogenesis were examined by evaluating their inhibitory
effects on UVA-induced cellular oxidative stress and modulation of antioxidant defenses including antioxidant enzymes, catalase
(CAT) and glutathione peroxidase (GPx), and intracellular glutathione (GSH). In addition, possible active compounds accountable
for biological activities of the extracts were identified by thin layer chromatography (TLC)-densitometric analysis. Our study
demonstrated that UVA (8 J/cm2) induced both tyrosinase activity and mRNA levels and UVA (16 J/cm2)-mediated melanin production were suppressed by the AG or CA extracts at noncytotoxic concentrations. Both extracts were
able to protect against UVA-induced cellular oxidant formation and depletion of CAT and GPx activities and GSH content in
a dose-dependent manner. Moreover, TLC-densitometric analysis detected the presence of eugenol and curcuminoids in AG and
CA, respectively. This is the first report representing promising findings on AG and CA extract-derived antityrosinase properties
correlated with their antioxidant potential. Inhibiting cellular oxidative stress and improving antioxidant defenses might
be the mechanisms by which the extracts yield the protective effects on UVA-dependent melanogenesis. 相似文献
8.
9.
Sukanya Luang Jung-Il Cho Bancha Mahong Rodjana Opassiri Takashi Akiyama Kannika Phasai Juthamath Komvongsa Nobuhiro Sasaki Yan-ling Hua Yuki Matsuba Yoshihiro Ozeki Jong-Seong Jeon James R. Ketudat Cairns 《The Journal of biological chemistry》2013,288(14):10111-10123
Glycosylation is an important mechanism of controlling the reactivities and bioactivities of plant secondary metabolites and phytohormones. Rice (Oryza sativa) Os9BGlu31 is a glycoside hydrolase family GH1 transglycosidase that acts to transfer glucose between phenolic acids, phytohormones, and flavonoids. The highest activity was observed with the donors feruloyl-glucose, 4-coumaroyl-glucose, and sinapoyl-glucose, which are known to serve as donors in acyl and glucosyl transfer reactions in the vacuole, where Os9BGlu31 is localized. The free acids of these compounds also served as the best acceptors, suggesting that Os9BGlu31 may equilibrate the levels of phenolic acids and carboxylated phytohormones and their glucoconjugates. The Os9BGlu31 gene is most highly expressed in senescing flag leaf and developing seed and is induced in rice seedlings in response to drought stress and treatment with phytohormones, including abscisic acid, ethephon, methyljasmonate, 2,4-dichlorophenoxyacetic acid, and kinetin. Although site-directed mutagenesis of Os9BGlu31 indicated a function for the putative catalytic acid/base (Glu169), catalytic nucleophile residues (Glu387), and His386, the wild type enzyme displays an unusual lack of inhibition by mechanism-based inhibitors of GH1 β-glucosidases that utilize a double displacement retaining mechanism. 相似文献
10.