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Theeranun Janjarasskul Kanitha Tananuwong Manusawee Leuangsukrerk Thunyaluck Phupoksakul Chaleeda Borompichaichartkul 《Food biophysics》2018,13(1):49-59
Impact of drying process and storage conditions on properties of konjac glucomannan (KGM) and whey protein isolate (WPI) blend films was investigated. Hundred grams of film solution contained 0.4 g KGM, 3.8 g WPI and 1.5 g glycerol. During drying process, air velocity was varied to produce fast drying (3 h) and slow drying (15 h) in tray dryers under 50 °C. The high air velocity resulted in a significant higher drying rate in fast drying than low air velocity in slow drying. Drying curves from both processes were well-fitted with Page model and Henderson and Pabis model (R2 ≥ 0.98). Fast drying improved transparency and mechanical properties without impairing color, solubility or water vapor permeability (WVP). Fast-dried film had less surface roughness and contained larger protein clusters. It also had greater melting enthalpy of protein aggregates, implying stronger networks. For stability study, fast-dried film was stored at 4-35 °C for 24 days. Transparency decreased over time. Overall mechanical properties have improved during storage. Color, solubility and WVP did not significantly change over time at all conditions (p?>?0.05). Microstructure of aged films was relatively similar to that of the freshly prepared film. Overall, the fast-dried KGM-WPI film exhibited reasonable storage stability. 相似文献
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Selvarathinam Kanitha Thekkumalai Malarvili Perumalsamy Balaji Vilwanathan Ravikumar 《International journal of peptide research and therapeutics》2021,27(3):1849-1860
International Journal of Peptide Research and Therapeutics - Antimicrobial peptides which play a vital role in an innate immune defense mechanism of various organisms can be regarded as novel... 相似文献
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Arun Buaklin Tanapat Palaga Drew Hannaman Ruthairat Kerdkaew Kanitha Patarakul Alain Jacquet 《Molecular biotechnology》2014,56(10):903-910
Bacterial outer membrane lipoproteins represent potent immunogens for the design of recombinant subunit vaccines. However, recombinant lipoprotein production and purification could be a challenge notably in terms of expression yield, protein solubility, and post-translational acylation. Together with the cost effectiveness, facilitated production, and purification as well as good stability, DNA-based vaccines encoding lipoproteins could become an alternative strategy for antibacterial vaccinations. Although the immunogenicity and the efficacy of DNA-based vaccines can be demonstrated in small rodents, such vaccine candidates could request concrete optimization as they are weak immunogens in primates and humans and particularly when administered by conventional injection. Therefore, the goal of the present study was to optimize the immunogenicity of a DNA vaccine encoding an outer membrane lipoprotein. LipL32, the major outer membrane protein from pathogenic Leptospira, was selected as a model antigen. We evaluated the influence of antigen secretion, the in vivo DNA delivery by electroporation, the adjuvant co-administration, as well as the heterologous prime-boost regimen on the induction of anti-LipL32 specific immune responses. Our results clearly showed that, following transfections, a DNA construct based on the authentic full-length LipL32 gene (containing leader sequence and the N-terminus cysteine residue involved in the protein anchoring) drives antigen secretion with the same efficiency as a plasmid-encoding anchor-less LipL32 and for which the bacterial leader sequence was replaced with a viral signal peptide. The in vivo DNA delivery by electroporation drastically enhanced the production of strong Th1 responses characterized by specific IgG2a antibodies and the IFNγ secretion in a restimulation assay, regardless of the DNA constructs used. In comparison with the heterologous prime-boost regimen, the homologous prime-boost vaccinations with DNA co-administrated with polyinosinic-polycytidylic acid (poly I:C) generated the highest specific IgG and IgG2a titers as well as the greatest IFNγ production. Taken together, these data suggest that optimization of outer membrane lipoprotein secretion is not critical for the induction of antigen-specific responses through DNA vaccination. Moreover, the potent antibody response induced by DNA plasmid encoding lipoprotein formulated with poly I:C and delivered through electroporation provides the rationale for the design of new prophylactic vaccines against pathogenic bacteria. 相似文献
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Teerasit Techawiwattanaboon Praparat Thaibankluay Chahya Kreangkaiwal Suwitra Sathean-Anan-Kun Prasong Khaenam Jiradej Makjaroen Trairak Pisitkun Kanitha Patarakul 《PLoS neglected tropical diseases》2021,15(11)
Leptospirosis is a re-emerging zoonosis with a global distribution. Surface-exposed outer membrane proteins (SE-OMPs) are crucial for bacterial–host interactions. SE-OMPs locate and expose their epitope on cell surface where is easily accessed by host molecules. This study aimed to screen for surface-exposed proteins and their abundance profile of pathogenic Leptospira interrogans serovar Pomona. Two complementary approaches, surface biotinylation and surface proteolytic shaving, followed by liquid chromatography tandem-mass spectrometry (LC-MS/MS) were employed to identify SE-OMPs of intact leptospires. For quantitative comparison, in-depth label-free analysis of SE-OMPs obtained from each method was performed using MaxQuant. The total number of proteins identified was 1,001 and 238 for surface biotinylation and proteinase K shaving, respectively. Among these, 39 were previously known SE-OMPs and 68 were predicted to be localized on the leptospiral surface. Based on MaxQuant analysis for relative quantification, six known SE-OMPs including EF- Tu, LipL21, LipL41, LipL46, Loa22, and OmpL36, and one predicted SE-OMPs, LipL71 were found in the 20 most abundant proteins, in which LipL41 was the highest abundant SE-OMP. Moreover, uncharacterized LIC14011 protein (LIP3228 ortholog in serovar Pomona) was identified as a novel predicted surface βb-OMP. High-abundance leptospiral SE-OMPs identified in this study may play roles in virulence and infection and are potential targets for development of vaccine or diagnostic tests for leptospirosis. 相似文献
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Background
Leptospirosis is a zoonosis of worldwide distribution caused by infection with pathogenic serovars of Leptospira spp. The most common species, L. interrogans, can survive in the environment for lengthy periods of time in between infection of mammalian hosts. Transmission of pathogenic Leptospira to humans mostly occurs through abraded skin or mucosal surfaces after direct or indirect contact with infected animals or contaminated soil or water. The spirochete then spreads hematogenously, resulting in multi-organ failure and death in severe cases. Previous DNA microarray studies have identified differentially expressed genes required for adaptation to temperature and osmolarity conditions inside the host compared to those of the environment. 相似文献
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