排序方式: 共有8条查询结果,搜索用时 15 毫秒
1
1.
Caryl A. Chlan Katyna Borroto J. A. Kamalay Leon Dure III 《Plant molecular biology》1987,9(6):533-546
The globulin storage protein genes of cotton are found to exist as gene tandems that contain a gene from each of the 2 globulin subfamilies separated by a spacer region of about 2700 or 3400 base pairs. Three different tandems have been identified by restriction endonuclease mapping of genomic DNA. A cDNA that is different from the genes of the tandems in map sites and/or in nucleotide sequence indicates that a fourth tandem probably exists in the cotton genome. Since the species of cotton used here (Gossypium hirsutum) is an amphidiploid, it is likely that two of the tandems are contributed from each genome.Considerable divergence in nucleotide sequence (18%) and in derived amino acid sequence (28%) is found when the 2 genes of a sequenced tandem are compared. The sequence of the cDNA closely resembles one of the genes in the tandem showing only a 4% divergence in nucleotides and a 4.2% divergence in amino acids. Thus the 2 genes of each tandem represent a relatively ancient gene duplication that has given rise to the two globulin subfamilies of cotton. Only one subfamily has a glycosylation site and the glycosylation of its derived proteins gives rise to the 2 molecular weight sets of globulins seen on gel electrophoresis.Other basic features of these genes and their derived proteins are presented. 相似文献
2.
3.
Regulation of structural gene expression in tobacco 总被引:1,自引:0,他引:1
4.
A library of complementary DNA (cDNA) clones has been prepared from poly(A)+RNA of spores of the sensitive fern, Onoclea sensibilis L. By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.Abbreviations bp
base pairs
- cDNA
complementary DNA
- ds
double-stranded
- ELIP
early light-inducible proteins
- LEA
late embryogenesis abundant
- nt
nucleotide
- ss
single stranded
This work was partially supported by a NASA grant (NAGW-901) and by an allocation from the Research Challenge Investigators' Fund of the Ohio State University to V.R. Thanks are due to Mr. Clayton L. Rugh for sequencing our clones and to Dr. Paul A. Fuerst for help in the computer search of sequence alignments. 相似文献
5.
Eshita Steven M. Kamalay Joseph C. Gingas Vicki M. Yaussy Daniel A. 《Plant Cell, Tissue and Organ Culture》2000,61(3):245-249
Cell suspension cultures of Dutch elm disease (DED)-tolerant and DED-susceptible American elms clones have been established
and characterized as prerequisites for contrasts of cellular responses to pathogen-derived elicitors. Characteristics of cultured
elm cell growth were monitored by A700 and media conductivity. Combined cell growth data for all experiments within a genotype showed relatively low variances and
between-genotype contrasts during repeated passages showed no significant differences. Subculturing exponentially growing
cells at 8–14 day intervals, within readily measured parameters of media conductivity (4.95–4.2 mmhos) and cell concentration
(≥ 1.4 A700), consistently resulted in repeatable profiles of elm cell growth and minimized lag phase. Culture cells were essentially
homogeneous after 5 subculture passages and their overall appearance was stable. We conclude that the described procedure
resulted in consistent cultures suitable for elicitor treatment experiments.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
6.
The genome of Crassostrea virignica, the American oyster, has been studied by reassociation kinetics in order to construct a profile of DNA sequence frequency components. Oyster DNA has been shown to contain at least 51% single copy DNA sequences and two classes of middle repetitive DNA. The major repetitive class contains sequences which are repeated on the average 20 times and comprise 29% of oyster DNA. The other class represents 10% of oyster DNA and contains sequences repeated approx. 3000 times. In addition the DNA of oyster contains at least 1% foldback sequences. The spectrum of DNA repetition components in the American oyster is similar to that found in the genomes of other mollusks. 相似文献
7.
RNA and DNA isolation from recalcitrant plant tissues. 总被引:9,自引:0,他引:9
8.
Kui Shin Voo Clayton L. Rugh Joe C. Kamalay 《In vitro cellular & developmental biology. Plant》1991,27(3):117-124
Summary We describe a tissue culture procedure for somatic embryogenesis and plantlet regeneration in cotton (Gossypium hirsutum L. cv. Coker 312). Callused explants or individual globular embryos were transferred to basal media to induce somatic embryogenesis.
To determine characteristic early indicators of successful germination and conversion, we identified six types of embryos
that developed on basal media. Two of the six embryo types, designated as tulip-shaped and trumpet-shaped, could undergo conversion
in preliminary tests, whereas the others had little or no developmental potential. Several media treatments designed to enhance
the maturation of globular somatic embryos failed to increase the fraction of embryos which matured to form recoverable types.
In efforts to improve plantlet recovery, tulip-shaped embryos were used in limited trials to contrast the effects of chemical
and physical desiccation treatments on germination and conversion. The selective use of tulip-shaped somatic embryos, coupled
with partial desiccation, seems to have augmented plant recovery. Growth habit, flowering, seed set, and lint production of
most of the regenerated plants were comparable to seed-derived plants grown under the same conditions.
Partial research support was provided by state and federal funds appropriated to the Ohio Agricultural Research and Development
Center, The Ohio State University. 相似文献
1