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B P Sokolov V V Dzhemelinski? V N Kalinin 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1989,(6):45-46
The simple technique for isolation of high-molecular eucaryotic DNA has been proposed. It includes cell or nuclei lysates by sodium dodecylsulfate in the presence of pronase, proteins precipitation by potassium acetate, DNA precipitation by ethanol. The DNA isolated by this technique is easily cleaved by restriction endonucleases and can be used for analysis of the unique genes by blot-hybridization. The yield of DNA is similar or somewhat higher than that in case of using the standard methods including phenol extraction or phenol-chloroform extraction. 相似文献
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A mathematical model of changes in histamine concentration in the wall of human bronchiole was constructed. The parameters of the model adequately characterize the state of patients with bronchial asthma. 相似文献
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Summary Mutagenic action of 60Co -rays on extracellular phages red
+ and red1 13 after irradiation in 4% nutrient broth in the absence or in the presence of 0.1 M cysteamine or in dried samples was studied. The yield of c mutations was almost independent of the repair genotype of the host cells (uvrA6, polA1, recA13, lexA102, uvrE502, uvrD3 or xthA9), of the phage Red function and of the conditions of -irradiation and was 1·10-12 per base pair and 1 rad. When the SOS-repair system of the host cells was induced by moderate UV irradiation, the yield of c-mutations was drastically enhanced in phage irradiated in broth, but not in phage irradiated in the dried state. These data allow us to suppose that the direct action of -rays induces, in phage DNA, premutational lesions that are fixed into mutations by replication. On the other hand after -irradiation in broth, when indirect radiation effects are only partially suppressed, about 85% of premutational lesions are converted into mutations by means of the inducible, errorprone SOS-repair system. 相似文献
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The restriction fragment length polymorphisms (RFLPs) of 3 collagen genes have been studied in two USSR populations. Four high-frequency alleles, also reported in other populations, were observed. The frequencies of alleles for the polymorphic sites MspI and EcoRI of the COL1A2 gene, HindIII of the COL2A1 gene and EcoRI of the COL3A1 gene were found to be 0.836, 0.398, 0.447 and 0.786, respectively, in Russians from Moscow (Caucasians), and 0.883, 0.519, 0.648 and 0.809, respectively, in Buryats from the Baikal region (Mongoloids). The RFLPs showed a fair agreement with the Hardy-Weinberg equilibrium. The frequencies were compared with those of other populations. 相似文献
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Lyme borreliosis in Lithuania: its prevalence, clinical picture and treatment efficacy 总被引:1,自引:0,他引:1
L I Moteiunas A R Shadzhene S V Tumosene M I Kalinin S V Dau?tas G K Regalene 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1990,(7):19-23
In 1988 Lyme borreliosis (LA) was diagnosed in 376 patients in Lithuania, including 101 patients who had the first stage of circular migrating erythema. Of this latter group, in 40 patients (39.6%) specific antibodies to the causative agent of LB were detected. All 101 patients were intensively treated with antibiotics for 10-14 days. The effectiveness of treatment was evaluated 4-5 months later. Information was collected only on 90 patients, including 82 patients who had undergone treatment. Complete convalescence was achieved in 52 cases (63.4%). 20 more persons considered themselves to be practically healthy. But in 10 patients (12.2%) the development of the disease continued, among them in 6 patients who stopped the prescribed course of treatment on their own accord. Out of 8 patients who had not yet even started to undergo treatment, 5 patients had signs of the manifest progress of the disease. Besides, information on the incubation period of the disease, its seasonal character, the characteristic features of erythema and concomitant symptoms in presented. 相似文献
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Abramov O. N. Aleksandrov V. V. Volkov G. S. Volobuev I. V. Grabovsky E. V. Gritsuk A. N. Zhigalov D. V. Kalinin Yu. G. Korolev V. D. Laukhin Ya. N. Medovshchikov S. F. Mitrofanov K. N. Oleinik G. M. Smirnova E. A. Frolov I. N. 《Plasma Physics Reports》2020,46(10):967-977
Plasma Physics Reports - Compression of a fiber array with a deuterated target mounted on its axis is studied at the Angara-5-1 facility (3.5 MA, 100 ns). Cylindrical arrays with an initial... 相似文献
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