Isolation of high molecular weight eukaryotic DNA with the use of potassium acetate

The simple technique for isolation of high-molecular eucaryotic DNA has been proposed. It includes cell or nuclei lysates by sodium dodecylsulfate in the presence of pronase, proteins precipitation by potassium acetate, DNA precipitation by ethanol. The DNA isolated by this technique is easily cleaved by restriction endonucleases and can be used for analysis of the unique genes by blot-hybridization. The yield of DNA is similar or somewhat higher than that in case of using the standard methods including phenol extraction or phenol-chloroform extraction.     

Mathematical model of histamine bronchospasm]

A mathematical model of changes in histamine concentration in the wall of human bronchiole was constructed. The parameters of the model adequately characterize the state of patients with bronchial asthma.     

Restriction fragment length polymorphisms of three collagen genes in Russians and Buryats.

The restriction fragment length polymorphisms (RFLPs) of 3 collagen genes have been studied in two USSR populations. Four high-frequency alleles, also reported in other populations, were observed. The frequencies of alleles for the polymorphic sites MspI and EcoRI of the COL1A2 gene, HindIII of the COL2A1 gene and EcoRI of the COL3A1 gene were found to be 0.836, 0.398, 0.447 and 0.786, respectively, in Russians from Moscow (Caucasians), and 0.883, 0.519, 0.648 and 0.809, respectively, in Buryats from the Baikal region (Mongoloids). The RFLPs showed a fair agreement with the Hardy-Weinberg equilibrium. The frequencies were compared with those of other populations.     

Lyme borreliosis in Lithuania: its prevalence, clinical picture and treatment efficacy

In 1988 Lyme borreliosis (LA) was diagnosed in 376 patients in Lithuania, including 101 patients who had the first stage of circular migrating erythema. Of this latter group, in 40 patients (39.6%) specific antibodies to the causative agent of LB were detected. All 101 patients were intensively treated with antibiotics for 10-14 days. The effectiveness of treatment was evaluated 4-5 months later. Information was collected only on 90 patients, including 82 patients who had undergone treatment. Complete convalescence was achieved in 52 cases (63.4%). 20 more persons considered themselves to be practically healthy. But in 10 patients (12.2%) the development of the disease continued, among them in 6 patients who stopped the prescribed course of treatment on their own accord. Out of 8 patients who had not yet even started to undergo treatment, 5 patients had signs of the manifest progress of the disease. Besides, information on the incubation period of the disease, its seasonal character, the characteristic features of erythema and concomitant symptoms in presented.     

Study of the Compression Dynamics of a Fiber Liner with a Deuterated Target Mounted on the Axis

Plasma Physics Reports - Compression of a fiber array with a deuterated target mounted on its axis is studied at the Angara-5-1 facility (3.5 MA, 100 ns). Cylindrical arrays with an initial...     

Effects of emotional expression of an irrelevant face image on recognition of visual stimuli

Psychophysiological experiments were performed on 34 healthy subjects. We analyzed the accuracy and latency of motor response in recognizing two types of complex visual stimuli, animals and objects, which were presented immediately after a brief presentation of face images with different emotional expressions: anger, fear, happiness, and a neutral expression. We revealed the dependence of response latency on emotional expression of the masked face. The response latency was lower when the test stimuli were preceded by angry or fearful faces compared to happy or neutral faces. These effects depended on the type of stimulus and were more expressive when recognizing objects compared to animals. We found that the effects of emotional faces were related to personal features of the subjects that they exhibited in the emotional and communicative blocks of Cattell’s test and were more expressive in more sensitive, anxious, and pessimistic introverts. The mechanisms of the effects of unconsciously perceived emotional information on human visual behavior are discussed.     

Multicomponent chemistry in the synthesis of carbonic anhydrase inhibitors

Abstract

Carbonic anhydrase inhibitors (CAIs) are of growing interest since various isoforms of the enzyme are identified as promising drug targets for treatment of disease. The principal drawback of the clinically used CAIs is the lack of isoform selectivity, which may lead to observable side effects. Studies aiming at the design of isoform-selective CAIs entail generation and biological testing of arrays of compounds, which is a resource- and time-consuming process. Employment of multicomponent reactions is an efficient synthetic strategy in terms of gaining convenient and speedy access to a range of scaffolds with a high degree of molecular diversity. However, this powerful tool appears to be underutilized for the discovery of novel CAIs. A number of studies employing multicomponent reactions in CAI synthesis have been reported in literature. Some of these reports provide inspiring examples of successful use of multicomponent chemistry to construct novel potent and often isoform-selective inhibitors. On critical reading of several publications, however, it becomes apparent that for some chemical series designed as CAIs, the desired inhibitory properties are only assumed and never tested for. In these cases, the biological profile is reported based on the results of phenotypical cellular assays, with no correlation with the intended on-target activity. Present review aims at critically assessing the current literature on the multicomponent chemistry in the CAI design.     

Spectroscopic measurements of the parameters of the helium plasma jets generated in the plasma focus discharge at the PF-3 facility

The spectroscopic technique used to measure the parameters of the plasma jets generated in the plasma focus discharge and those of the plasma of the immobile gas through which these jets propagate is described. The time evolution of the intensities and shapes of spectral lines in experiments carried out with helium at the PF-3 facility was studied by means of electron-optical streak cameras. The plasma electron temperature, T ≈ 4–5 eV, was determined from the intensity ratio of two spectral lines, one of which (λ₁ = 5876 Å) belongs to neutral helium, while the other (λ₂ = 4686 Å), to hydrogen-like helium ions. The plasma density at different time instants was determined from the Stark broadening of these lines in the electric fields of different nature. The plasma density is found to vary from 4 × 10¹⁴ to 2 × 10¹⁷ cm^?3.