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Transient Cytoplasmic pH Changes in Correlation with Opening of Potassium Channels in Eremosphaera 总被引:1,自引:0,他引:1
Steigner, W. Khler, K., Simonis, W. and Urbach, W. 1988. Transientcytoplasmic pH changes in correlation with opening of potassiumchannels in Eremosphaera.J. exp. Bot. 39: 2336. The role of the cytoplasmic pH (pHc) of Eremosphaera viridisin the signal transduction chain after light-off from the chloroplaststo the K+ channels in the plasmalemma of this unicellular algawas investigated. The temporary opening of K+ channels is indicatedby a transient hypcrpolarization (TP). To record rapid changesof pHc, continuous measurements with pH sensitive micro-electrodeswere carried out. (i) Under normal conditions pHc in the light(7·56 ±0·2) did not differ from pHc inthe dark (7·62 ±0·2). (ii) The vacuolepH ranged between 4·8 and 5·2. (iii) After light-offa rapid transient acidification of pHc O19±0·07occurred and a TP was released, (iv) In every case, the startof the transient acidification after light-off preceded thehyperpolarization by about 3s. (v) Light-on caused a rapid transientalkalinization but never a TP. (vi) Change to acid externalmedium (3.2) transiently acidified the cytoplasm and was ableto release a TP. (vii) After addition of NH4Cl, pHc again showeda rapid transient acidification and the release of a TP. The origin of the protons appearing in the cytoplasm after light-offis discussed critically with respect to the buffer capacity.Either direct or indirect translocation is a possible mechanismfor the movement of H+ from the chloroplasts into the cytoplasm.The intracellular acidification and its relation to the openingof potassium channels in the plasmalemma leads us to suggestthat a sudden change of pHc is a potent internal signal factorin Eremosphaera viridis. Key words: Cytoplasmic pH, transient potential, K+channels, Eremosphaera viridis 相似文献
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MICHAEL WILEY STEVEN KOHLER & PAUL SEELBACH 《The Plant journal : for cell and molecular biology》2004,37(1):133-148
1. Rapidly advancing geographical information systems (GIS) technologies are forcing a careful evaluation of the roles and biases of landscape and traditional site-based perspectives on assessments of aquatic communities. Viewing the world at very different scales can lead to seeming contradictions about the nature of specific ecological systems. In the case of Michigan trout streams, landscape analyses suggest a predictable community shaped by large-scale patterns in hydrology and geology. Most site-based studies, however, suggest these communities are highly variable in structure over time, and are strongly shaped by site-specific physical and biological dynamics. As the real world is comprised of processes operating both at local and landscape scales, an analytical framework for integrating these paradigms is desirable.
2. Decomposition of variances by factorial ANOVA into time, space and time–space interaction terms can provide a conceptual and analytical model for integrating processes operating at landscape and local scales. Using this approach, long-term data sets were examined for three insects and two fishes common in Michigan trout streams. Each taxon had a unique variance structure, and the observed variance structure was highly dependent upon sample size.
3. Both spatially extensive designs with little sampling over time (typical of many GIS studies) and temporally extensive designs with little or no spatial sampling (typical of population and community studies), are biased in terms of their view of the relative importance of local and landscape factors. The necessary, but in many cases costly, solution is to develop and analyse data sets that are both spatially and temporally extensive. 相似文献
2. Decomposition of variances by factorial ANOVA into time, space and time–space interaction terms can provide a conceptual and analytical model for integrating processes operating at landscape and local scales. Using this approach, long-term data sets were examined for three insects and two fishes common in Michigan trout streams. Each taxon had a unique variance structure, and the observed variance structure was highly dependent upon sample size.
3. Both spatially extensive designs with little sampling over time (typical of many GIS studies) and temporally extensive designs with little or no spatial sampling (typical of population and community studies), are biased in terms of their view of the relative importance of local and landscape factors. The necessary, but in many cases costly, solution is to develop and analyse data sets that are both spatially and temporally extensive. 相似文献
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