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Kang Hee-Kwon Jang Jun-Hyuck Shim Jae-Hoon Park Jong-Tae Kim Young-Wan Park Kwan-Hwa 《World journal of microbiology & biotechnology》2010,26(10):1915-1918
4-α-Glucanotransferases possess strong transglycosylation activity which has been used in various carbohydrate chemistry fields.
Due to safety issues of the recombinant enzymes we chose Bacillus subtilis as an expression host to produce a thermostable 4-α-glucanotransferase from Thermus scotoductus (TSαGT). The HpaII promoter in the Gram-positive bacterial vector pUB110 was used first to express TSαGT gene in B. subtilis. However, the activity of TSαGT in B. subtilis was only 4% of that in our previous Escherichia coli system. Two expression systems constructed by sequential alignment of another constitutive promoter for either α-amylase
from B. subtilis NA64 or maltogenic amylase from Bacillus licheniformis downstream of the HpaII promoter elevated the TSαGT productivity by 11- and 12-fold, respectively, compared to the single HpaII promoter system. In conclusion, the dual promoter systems in this study were much better than the single promoter system
to express the TSαGT gene in B. subtilis. 相似文献
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