全文获取类型
收费全文 | 2975篇 |
免费 | 265篇 |
出版年
2023年 | 25篇 |
2022年 | 56篇 |
2021年 | 89篇 |
2020年 | 66篇 |
2019年 | 69篇 |
2018年 | 89篇 |
2017年 | 64篇 |
2016年 | 119篇 |
2015年 | 196篇 |
2014年 | 202篇 |
2013年 | 268篇 |
2012年 | 321篇 |
2011年 | 255篇 |
2010年 | 184篇 |
2009年 | 163篇 |
2008年 | 159篇 |
2007年 | 168篇 |
2006年 | 138篇 |
2005年 | 108篇 |
2004年 | 90篇 |
2003年 | 87篇 |
2002年 | 51篇 |
2001年 | 22篇 |
2000年 | 19篇 |
1999年 | 16篇 |
1998年 | 7篇 |
1997年 | 10篇 |
1996年 | 8篇 |
1994年 | 5篇 |
1993年 | 7篇 |
1992年 | 22篇 |
1991年 | 7篇 |
1990年 | 11篇 |
1989年 | 7篇 |
1988年 | 13篇 |
1987年 | 10篇 |
1986年 | 8篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 9篇 |
1982年 | 8篇 |
1981年 | 5篇 |
1980年 | 8篇 |
1978年 | 7篇 |
1976年 | 4篇 |
1975年 | 11篇 |
1972年 | 3篇 |
1971年 | 4篇 |
1969年 | 3篇 |
1937年 | 3篇 |
排序方式: 共有3240条查询结果,搜索用时 31 毫秒
1.
C Julien C Barres P Kandza J Sassard 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》1989,308(8):219-222
Intra-aortic blood pressure (BP) was measured in conscious rats after early chronic destruction of the peripheral sympathetic nervous system (SNS) with guanethidine. In sympathectomized rats, the mean level of BP was not different from that of control rats but its variability was markedly increased. These results indicate that functional integrity of the SNS is of primary importance for the short-term control of BP but is not essential for its long-term maintenance. 相似文献
2.
3.
4.
5.
A new human species of aldolase A mRNA from fibroblasts 总被引:2,自引:0,他引:2
P Izzo P Costanzo A Lupo E Rippa A M Borghese G Paolella F Salvatore 《European journal of biochemistry》1987,164(1):9-13
A full-length cDNA aldolase A clone was isolated from a human fibroblast cDNA library and completely sequenced. Excluding the poly(A) tail, the clone covers 1095 base pairs (bp) of the coding region, plus 199 bp downstream for the termination codon and 146 bp upstream for the initiation codon, within a total of 1440 bp. Primer extension experiments performed with human cultured fibroblast mRNA indicate an elongated product of a further 40 bp. These results evaluated together with those obtained in a concurrent study concerning aldolase A mRNA isolated from human liver are direct evidence of aldolase A mRNA multiplicity in man. The data also suggest the existence in mammals of three different classes of aldolase A mRNA, which would account for tissue specificity and resurgence of foetal expression in tumors. 相似文献
6.
7.
8.
A new fluorescent acridine orange derivative, 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA), has been applied to Hela cells in order to set up appropriate conditions for the detection of mycoplasma contaminations. Since AMHA staining reveals intensely fluorescent nuclei and slight fluorescent cytoplasm, we can visualize and localize mycoplasma contamination on each cell. In combination with a shortened Chen's staining method (1977), AMHA should allow a better detection of mycoplasma in animal cell cultures than the well established Hoechst dye. 相似文献
9.
10.
K99 lectin fromEscherichia coli was purified and biotinylated via its carboxyl groups using biocytin hydrazide and a water soluble carbodiimide. Biotinylation of two out of the nine carboxyl groups was sufficient to permit detection of the lectin by avidin and did not cause any loss of the haemagglutinating activity. It was demonstrated that the biotinylated K99 lectin retained other important properties of native K99 and that it will probably become a very sensitive detecting reagent. Indeed, it was able to bind to HeLa cells, as do intact bacteria carrying K99 fimbriae, and also to recognizeN-glycolyl-neuraminyl-lactosyl-ceramide in an overlay binding assay.
Abbreviations: NeuAc,N-acetylneuraminic acid; NeuGc,N-glycolylneuraminic acid; PBS, phosphate buffered saline (0.9% NaCl containing 150mm sodium phosphate, pH 7.2); LPS, lipopolysaccharide; BCHZ, biocytin hydrazide; EDC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide; BSA, bovine serum albumin; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; DMEM, Dulbecco's modified Eagle medium. For the gangliosides, trivial names and structures are given according to the recommendations in [43]. NeuAc2-3Gal1-4Glc1-1Cer (NeuAc-GM3); NeuGc2-3Gal1-4Glc1-1Cer (NeuGc-GM3); GalNAc1-4(NeuAc2-3)Gal1-4Glc1-1Cer (GM2); NeuAc2-8NeuAc2-3Gal1-4Glc1-1Cer (GD3); Gal1-3GalNAc1-4(NeuAc2-3)Gal1-4Glc1-1Cer (GM1); NeuAc2-3Gal1-3GalNAc1-4(NeuAc2-3)Gal1-4Glc1-1Cer (GD1a); Gal1-3GalNAc1-4(NeuAc2-8NeuAc2-3)Gal1-4Gle1-1Cer (GD1b); NeuAc2-3Gal1-3GalNAc1-4(NeuAc2-8NeuAc2-3) Gal1.-4Glc1-1Cer (GT1b). NeuGc2-3Gal1-4GleNAc1-4Gal1-4Glc1-1Cer (NeuGc-SPG). 相似文献