全文获取类型
收费全文 | 5364篇 |
免费 | 329篇 |
专业分类
5693篇 |
出版年
2024年 | 8篇 |
2023年 | 34篇 |
2022年 | 106篇 |
2021年 | 164篇 |
2020年 | 135篇 |
2019年 | 138篇 |
2018年 | 205篇 |
2017年 | 173篇 |
2016年 | 230篇 |
2015年 | 356篇 |
2014年 | 373篇 |
2013年 | 426篇 |
2012年 | 477篇 |
2011年 | 472篇 |
2010年 | 278篇 |
2009年 | 231篇 |
2008年 | 267篇 |
2007年 | 274篇 |
2006年 | 225篇 |
2005年 | 174篇 |
2004年 | 148篇 |
2003年 | 127篇 |
2002年 | 126篇 |
2001年 | 75篇 |
2000年 | 76篇 |
1999年 | 50篇 |
1998年 | 30篇 |
1997年 | 23篇 |
1996年 | 22篇 |
1995年 | 15篇 |
1994年 | 9篇 |
1993年 | 14篇 |
1992年 | 20篇 |
1991年 | 27篇 |
1990年 | 16篇 |
1989年 | 15篇 |
1988年 | 11篇 |
1987年 | 12篇 |
1986年 | 16篇 |
1985年 | 6篇 |
1984年 | 15篇 |
1983年 | 6篇 |
1981年 | 5篇 |
1980年 | 7篇 |
1979年 | 6篇 |
1977年 | 6篇 |
1975年 | 7篇 |
1974年 | 8篇 |
1973年 | 8篇 |
1972年 | 8篇 |
排序方式: 共有5693条查询结果,搜索用时 0 毫秒
1.
Hugo R. Permingeat Maria V. Romagnoli Juliana I. Sesma Ruben H. Vallejos 《Plant Molecular Biology Reporter》1998,16(1):89-89
An easy, reproducible and fast procedure to isolate DNA from cotton leaves is described. The addition of 0.5 M glucose in the extraction buffer avoids browning by polyphenolic compounds and improves the quality of DNA for molecular analysis. The DNA yield ranged between 150–400 mg per gram of fresh tissue. The DNA was suitable for digestion by restriction enzymes and amplificatiion by Taq DNA polymerase. 相似文献
2.
The involvement of gibberellins in the control of flowering of sunflower was studied by direct application of GA3 to the apex of the plants, analysis of the endogenous levels of gibberellin-like substances at different plant ages, and indirectly by the application of paclobutrazol, an inhibitor of gibberellin synthesis. GA3 speeded-up flower initiation and floral apex development. The time of GA3 application was more critical than the amount of GA3 applied. The endogenous levels of gibberellin-like compounds increased significantly by day 15 after sowing. The application of paclobutrazol markedly delayed floral initiation and this effect was also depedent on plant age. Both GA3 and paclobutrazol had their greatest effects between 10 and 20 days after sowing suggesting that an increase in gibberellins in that time period plays a role in floral initiation. 相似文献
3.
4.
5.
6.
Almeida AP 《Theriogenology》1987,27(2):329-335
A comparison between different superovulatory treatments in dairy cattle was carried out at a commercial embryo transfer unit in Israel. Both pregnant mare serum gonadotrophin (PMSG) and follicle stimulating hormone (FSH) were used, either alone or combined with Syncromate B (SMB). The use of PMSG + SMB significantly decreased the number of corpora lutea present at the time of embryo collection 7 d after insemination, as compared with other treatment regimens. Consequently, a significantly lower number of ova was found in those animals treated with PMSG + SMB. Better superovulatory responses were obtained when FSH, rather than PMSG, was used, regardless of whether they were administered alone or combined with SMB. It was clear that the use of SMB combined either with PMSG or FSH resulted in poorer responses than when either gonadotrophin was used alone. 相似文献
7.
Summary Production of trace levels of carbon monoxide was consistently observed in the off-gas of a laboratory anaerobic digester fed Waste Activated Sludge. Inocula from this digester was enriched for acetate and methanol utilizing methanogenic populations. These enriched inocula were then monitored in batch assays for carbon monoxide and hydrogen production. Results demonstrated that carbon monoxide is produced during methanogenesis on both substrates. Subsequent utilization of CO was observed to occur after methane production was essentially complete for the assays conducted with methanol. Carbon monoxide evolution during methanogenesis on acetate displayed a markedly different trend from that observed from methanol. 相似文献
8.
G-11 staining in Turner's syndrome with mos 45,X/46,X,r(?) 总被引:2,自引:0,他引:2
J C de Almeida J C Llerena D Molina Gomes R Rita Martins M Jung D F Reis A G Cunha 《Annales de génétique》1985,28(1):37-41
Mos 45,X/46,X,r(?) in 4 patients with Turner's syndrome and no signs of virilization, and in one pair of monozygotic twins, one of them with clitoral hypertrophy, was studied using combined cytogenetic techniques and specially G-11 staining for the characterization of the X or Y origin of the rings. In all 6 patients the ring was G-11 positive, attesting its Y origin. Both twins were operated and bilateral streak gonads with a bilateral nodule of testicular tissue were found. Similar small rings were also studied in one patient with mos 46,XX/46,X,r(X) and in one nonvirilized Turner's syndrome patient with a larger ring; in these two cases the ring was G-11 negative. It seems that the small rings occasionally found in Turner's syndrome are more frequently from Y origin and therefore prophylactic gonadectomy should be considered. 相似文献
9.
I T Toledo e Souza B L Wajchenberg F Prestes Cesar J S Almeida Neto 《Hormones et métabolisme》1983,15(12):575-580
Insulin and C-peptide (free insulin and C-peptide in insulin-treated patients) were measured after glucose stimulation in nine Type II diabetics on chlorpropamide, eleven insulin-treated maturity-onset diabetics and in 8 normal controls. Dissociation between C-peptide and insulin response to glucose was observed in several diabetics. The relation between incremental molar areas under C-peptide and insulin curves, after glucose challenge (delta CPR - delta IRI/delta CPR) were used to evaluate the hepatic insulin extraction in all but the insulin-treated diabetics. The lower insulin requirements and better control of the short-duration insulin-treated maturity-onset diabetics in relation to the long-term ones could not be explained either by the residual insulin secretion or by the level of "insulin antibodies". The chlorpropamide-responsive patients presented higher insulin levels after the glucose challenge and a lower hepatic insulin extraction than the non-responsive ones. 相似文献
10.
Neil S. Painter Anthony Z. Almeida Kenneth W. Colebourne 《BMJ (Clinical research ed.)》1972,2(5806):137
Seventy patients with diverticular disease of the colon were treated with a high-residue, low-sugar diet including unprocessed bran. Follow-up for an average of 22 months showed marked relief of symptoms in 62 patients. Bowel habit was restored towards normal and abdominal discomfort relieved. Only seven patients were unable to give up the use of laxatives. Eight patients did not tolerate the bran diet, and one of these needed surgical treatment. None of the 62 patients who took the diet needed surgery. 相似文献